Collaborative Research: Teasing apart how specific nanoparticle features relate to environmental fate and contribute to ecotoxicity

合作研究：梳理特定纳米颗粒特征如何与环境命运相关并导致生态毒性

• 批准号: 1762278
• 负责人: Marilyn Mackiewicz
• 金额: $ 24.46万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-15 至 2021-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1762278&HistoricalAwards=false
• 关键词: Collaborative; Research; Teasing; apart; how

Silver nanoparticles are extensively used for their antimicrobial properties in an increasing number of consumer and commercial products as well as in wastewater treatment. Understanding the potential toxicity and environmental impacts of silver nanoparticles is challenging. Because silver nanoparticles undergo ionic dissolution, discrepancies exist over the relative contribution of silver ions or the nanoparticles themselves in toxicity. This project will increase understanding of how silver nanoparticle properties affect bio-uptake, nanoparticle-biological interactions, and ecotoxicity. Effects of particle surface oxidation and ionic dissolution, which have complicated toxicity studies in the past, will be eliminated. This study will identify properties of silver nanoparticles that make them ecologically disruptive leading to adverse environmental outcomes. All data will be shared through the open-source knowledge base of Nanomaterial-Biological Interactions (NBI) globally for modeling efforts and will support the development of safety protocols, exposure guidelines, and regulations that protect human and ecosystem health. Furthermore, this research will provide design rules for the assembly of new classes of silver nanoparticles that could be commercialized without concern regarding rapid particle degradation and release into the environment. In addition, this project is designed to incorporate students from diverse backgrounds and will help build future science, technology, engineering and math (STEM) talent.The researcher's overall aim is to improve our understanding of the specific physiochemical features that dictate nanoparticle-biological interactions. First, they will design a series of lipid-coated silver nanoparticles that are differentially shielded from ion dissolution. Differentially shielded silver nanoparticles will be prepared by encapsulating silver nanoparticles of varying size and shape with a hybrid lipid-membrane to protect the surface from oxidation and ionic dissolution. Changes in the localized surface plasmon resonance (LSPR), thermal electron microscope (TEM), and (Inductively-coupled plasma mass spectrometer (ICP-MS) will be employed to monitor silver ion dissolution from the suite of nanoparticles. Second, they will identify features of lipid-coated nanoparticles that lead to particle instability. The agglomeration kinetics of the hybrid lipid-coated silver nanoparticles will be assessed using dynamic light scattering and nanoparticle tracking analysis. Third, since the goal is to ultimately relate these material features with nanoparticle-biological interactions, the researchers will determine the uptake and toxicity of the silver nanoparticle suite. Based on preliminary investigations, the hybrid lipid-coated silver nanoparticles with a robust coating should elicit minimal toxicity and a decrease in surface coverage should lead to a respective increase in toxicity. A well-established embryonic zebrafish assay will be used to identify vertebrate morbidity and mortality resulting from exposure and hyperspectral imaging (HSI) will be used to visualize nanoparticle uptake in whole animals. Finally, the researchers will assess the potential ecotoxicity of the suite using a novel nanocosm assay. Hyperspectral imaging will be used to visualize nanoparticle biodistribution among bacteria, algae, crustaceans, and fish in the small-scale freshwater assay. Collectively, the use of well-characterized silver nanoparticles tuned for ion release will allow the PIs to tease apart the relative contribution of the nanoparticle and ion to biouptake, toxicity, and potential for environmental impacts.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

银纳米颗粒因其抗微生物性能而广泛用于越来越多的消费品和商业产品以及废水处理中。 了解银纳米颗粒的潜在毒性和环境影响是具有挑战性的。由于银纳米颗粒经历离子溶解，因此银离子或纳米颗粒本身在毒性方面的相对贡献存在差异。 这个项目将增加对银纳米粒子特性如何影响生物吸收、纳米粒子-生物相互作用和生态毒性的理解。 颗粒表面氧化和离子溶解的影响，这在过去的毒性研究复杂，将被消除。这项研究将确定银纳米粒子的性质，使他们生态破坏导致不利的环境后果。所有数据将通过全球纳米材料-生物相互作用（NBI）的开源知识库共享，用于建模工作，并将支持安全协议，暴露指南和保护人类和生态系统健康的法规的制定。此外，这项研究将提供设计规则的组装新类别的银纳米粒子，可以商业化，而无需考虑快速颗粒降解和释放到环境中。此外，该项目旨在融合来自不同背景的学生，并将有助于培养未来的科学，技术，工程和数学（STEM）人才。研究人员的总体目标是提高我们对决定纳米粒子生物相互作用的特定理化特征的理解。首先，他们将设计一系列脂质包覆的银纳米粒子，这些纳米粒子被不同程度地屏蔽了离子溶解。通过用混合脂质膜包封不同尺寸和形状的银纳米颗粒来制备差异屏蔽的银纳米颗粒，以保护表面免受氧化和离子溶解。局部表面等离子体共振（LSPR）、热电子显微镜（TEM）和电感耦合等离子体质谱仪（ICP-MS）的变化将用于监测银离子从纳米颗粒套件中的溶解。其次，他们将确定导致颗粒不稳定的脂质涂层纳米颗粒的特征。将使用动态光散射和纳米颗粒跟踪分析评估混合脂质包覆的银纳米颗粒的团聚动力学。第三，由于目标是最终将这些材料特征与纳米颗粒-生物相互作用联系起来，研究人员将确定银纳米颗粒套件的吸收和毒性。基于初步研究，具有坚固涂层的混合脂质涂覆的银纳米颗粒应引起最小的毒性，并且表面覆盖度的降低应导致相应的毒性增加。将使用成熟的胚胎斑马鱼测定来鉴定暴露导致的脊椎动物发病率和死亡率，并将使用高光谱成像（HSI）来可视化整个动物中的纳米颗粒摄取。最后，研究人员将使用一种新的纳米宇宙测定法评估该套件的潜在生态毒性。高光谱成像将用于在小规模淡水分析中可视化细菌，藻类，甲壳类动物和鱼类之间的纳米颗粒生物分布。总的来说，使用经过离子释放调整的良好表征的银纳米颗粒将允许PI梳理纳米颗粒和离子对生物量、毒性和潜在环境影响的相对贡献。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的知识价值和更广泛的影响审查标准进行评估来支持。