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Collaborative Research: Teasing apart how specific nanoparticle features relate to environmental fate and contribute to ecotoxicity

合作研究：梳理特定纳米颗粒特征如何与环境命运相关并导致生态毒性

基本信息

批准号：
2115080
负责人：
Marilyn Mackiewicz
金额：
$ 24.46万
依托单位：
Oregon State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2021
资助国家：
美国
起止时间：
2021-01-01 至 2022-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2115080&HistoricalAwards=false
关键词：
Collaborative Research Teasing apart how

项目摘要

Silver nanoparticles are extensively used for their antimicrobial properties in an increasing number of consumer and commercial products as well as in wastewater treatment. Understanding the potential toxicity and environmental impacts of silver nanoparticles is challenging. Because silver nanoparticles undergo ionic dissolution, discrepancies exist over the relative contribution of silver ions or the nanoparticles themselves in toxicity. This project will increase understanding of how silver nanoparticle properties affect bio-uptake, nanoparticle-biological interactions, and ecotoxicity. Effects of particle surface oxidation and ionic dissolution, which have complicated toxicity studies in the past, will be eliminated. This study will identify properties of silver nanoparticles that make them ecologically disruptive leading to adverse environmental outcomes. All data will be shared through the open-source knowledge base of Nanomaterial-Biological Interactions (NBI) globally for modeling efforts and will support the development of safety protocols, exposure guidelines, and regulations that protect human and ecosystem health. Furthermore, this research will provide design rules for the assembly of new classes of silver nanoparticles that could be commercialized without concern regarding rapid particle degradation and release into the environment. In addition, this project is designed to incorporate students from diverse backgrounds and will help build future science, technology, engineering and math (STEM) talent.The researcher's overall aim is to improve our understanding of the specific physiochemical features that dictate nanoparticle-biological interactions. First, they will design a series of lipid-coated silver nanoparticles that are differentially shielded from ion dissolution. Differentially shielded silver nanoparticles will be prepared by encapsulating silver nanoparticles of varying size and shape with a hybrid lipid-membrane to protect the surface from oxidation and ionic dissolution. Changes in the localized surface plasmon resonance (LSPR), thermal electron microscope (TEM), and (Inductively-coupled plasma mass spectrometer (ICP-MS) will be employed to monitor silver ion dissolution from the suite of nanoparticles. Second, they will identify features of lipid-coated nanoparticles that lead to particle instability. The agglomeration kinetics of the hybrid lipid-coated silver nanoparticles will be assessed using dynamic light scattering and nanoparticle tracking analysis. Third, since the goal is to ultimately relate these material features with nanoparticle-biological interactions, the researchers will determine the uptake and toxicity of the silver nanoparticle suite. Based on preliminary investigations, the hybrid lipid-coated silver nanoparticles with a robust coating should elicit minimal toxicity and a decrease in surface coverage should lead to a respective increase in toxicity. A well-established embryonic zebrafish assay will be used to identify vertebrate morbidity and mortality resulting from exposure and hyperspectral imaging (HSI) will be used to visualize nanoparticle uptake in whole animals. Finally, the researchers will assess the potential ecotoxicity of the suite using a novel nanocosm assay. Hyperspectral imaging will be used to visualize nanoparticle biodistribution among bacteria, algae, crustaceans, and fish in the small-scale freshwater assay. Collectively, the use of well-characterized silver nanoparticles tuned for ion release will allow the PIs to tease apart the relative contribution of the nanoparticle and ion to biouptake, toxicity, and potential for environmental impacts.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

银纳米粒子因其抗菌性能在越来越多的消费和商业产品中以及在废水处理中被广泛使用。了解银纳米颗粒的潜在毒性和环境影响是具有挑战性的。由于银纳米颗粒会发生离子溶解，因此在毒性方面，银离子的相对贡献或纳米颗粒本身的毒性存在差异。该项目将增加对纳米银颗粒特性如何影响生物吸收、纳米颗粒-生物相互作用和生态毒性的理解。颗粒表面氧化和离子溶解的影响将被消除，这在过去的毒性研究中是复杂的。这项研究将确定银纳米颗粒的特性，使它们对生态造成破坏，从而导致不利的环境后果。所有数据将通过全球纳米材料-生物相互作用(NBI)开源知识库共享，用于建模工作，并将支持制定保护人类和生态系统健康的安全协议、暴露指南和法规。此外，这项研究将为新型银纳米颗粒的组装提供设计规则，这些纳米颗粒可以商业化，而不必担心颗粒的快速降解和释放到环境中。此外，该项目旨在吸收来自不同背景的学生，并将有助于培养未来的科学、技术、工程和数学(STEM)人才。研究人员的总体目标是提高我们对决定纳米粒子-生物相互作用的特定物理化学特征的理解。首先，他们将设计一系列脂质包裹的银纳米颗粒，以不同的方式屏蔽离子溶解。通过将不同大小和形状的银纳米颗粒包裹在一种杂化的脂膜中来保护表面免受氧化和离子溶解，将得到不同屏蔽的银纳米颗粒。局域表面等离子体共振(LSPR)、热电子显微镜(TEM)和电感耦合等离子体质谱仪(ICP-MS)的变化将被用来监测银离子从该套纳米粒子中的溶解情况。其次，他们将识别导致颗粒不稳定的脂衣纳米颗粒的特征。利用动态光散射和纳米颗粒跟踪分析来评估杂化脂质包覆银纳米颗粒的团聚动力学。第三，由于目标是最终将这些材料特性与纳米颗粒-生物相互作用联系起来，研究人员将确定银纳米颗粒套件的摄取和毒性。根据初步研究，具有坚固涂层的杂化脂质包覆的银纳米颗粒应该会引起最小的毒性，表面覆盖率的减少应该会导致相应的毒性增加。一种成熟的胚胎斑马鱼试验将用于确定脊椎动物因暴露而导致的发病率和死亡率，高光谱成像(HSI)将用于可视化整个动物对纳米颗粒的摄取。最后，研究人员将使用一种新的纳米微粒试验来评估该套件的潜在生态毒性。高光谱成像将用于在小规模淡水测试中可视化纳米颗粒在细菌、藻类、甲壳类动物和鱼类中的生物分布。总体而言，使用针对离子释放进行调整的特性良好的银纳米颗粒将允许PI梳理纳米颗粒和离子对生物吸收、毒性和潜在的环境影响的相对贡献。这一奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。