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PFI:AIR - TT: Green, Biocompatible Enzymatic Disinfectants for Broad-spectrum Inhibition and Removal of Microbial Contamination in Packaged Produce

PFI:AIR - TT：绿色、生物相容性酶消毒剂，用于广谱抑制和去除包装产品中的微生物污染

基本信息

批准号：
1801612
负责人：
Bryan Berger
金额：
$ 19.96万
依托单位：
University of Virginia Main Campus
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2017
资助国家：
美国
起止时间：
2017-10-01 至 2019-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1801612&HistoricalAwards=false
关键词：
PFI AIR TT Green Biocompatible

项目摘要

This PFI: AIR Technology Translation project focuses on producing custom enzymes designed to break down polysaccharides which form the foundation of common microbial biofilms. The enzymes can be used in an aqueous solution that can be applied to food during cleaning and processing to remove biofilm-forming microbes. The use of a safe and green enzyme-based disinfectant is important because of the rising frequency of food-borne illness and associated outbreaks linked to microbial contamination from pathogens such as E. coli and Salmonella. In addition, these enzymes offer an opportunity to replace harsh chemical cleaning agents with biocompatible, safe and green alternatives. This project will result in a family of cleaning products formulated for a wide range of uses in cleaning of produce and other packaged foods. The uniqueness of the enzyme-based cleaning products are (1) ability to engineer specificity and target a broad range of microbial biofilms, (2) ability to select enzymes that retain activity under actual cleaning conditions, (3) ability to discriminate between pathogen and non-pathogen microbial biofilms, (4) demonstrated methods for high-yield production and (5) providing a safe, biocompatible alternative to current chemical cleaning agents. Collectively these features provide a new, green and targeted alternative to current industry standard chemical disinfectants such as peracetic acid in this market space. This project addresses key gaps in knowledge required to translate this technology to the marketplace. Chemical disinfectants and mechanical disruption often have limited efficacy against microbial biofilms, allowing potential pathogens to persist in processing environments despite cleaning and cause foodborne illness. Furthemore, biofilm chemical structure is often complex and heterogeneous due to the presence of multiple microbes present within a biofilm community. This technology addresses both of these gaps by engineering enzymes that can degrade chemically heterogeneous biofilms, and can act synergistically with other cleaning methods or alone to enhance removal of microbial pathogens present during food processing. In particular, the project utilizes some leading industry partners such as Highland Fresh Technologies who will assist in designing and simulating actual process conditions under which application testing can be performed to determine amount per use and method of application. Also, production costs during scale-up will be calculated to identify critical costs associated with the final product. Additionally, post-doctoral fellows and graduate students working on this project will receive technology translation expertise, working to answer critical questions necessary to identify production costs, business plan development as well as direct exposure to key industry partners to design and execute key testing of products.

该PFI：AIR技术翻译项目专注于生产定制酶，旨在分解多糖，这些多糖构成了常见微生物生物膜的基础。这些酶可以在水溶液中使用，该水溶液可以在清洁和加工过程中应用于食品以去除生物膜形成微生物。由于食源性疾病和与病原体如大肠杆菌的微生物污染有关的相关爆发的频率不断上升，因此使用安全和绿色酶基消毒剂非常重要。大肠杆菌和沙门氏菌。此外，这些酶提供了一个机会，以取代苛刻的化学清洁剂与生物相容性，安全和绿色的替代品。该项目将导致一个家庭的清洁产品，制定了广泛的用途，在清洁生产和其他包装食品。基于酶的清洁产品的独特性是（1）设计特异性并靶向广泛的微生物生物膜的能力，（2）选择在实际清洁条件下保持活性的酶的能力，（3）区分病原体和非病原体微生物生物膜的能力，（4）用于高产率生产的经证实的方法，以及（5）提供安全，目前化学清洁剂的生物相容性替代品。总的来说，这些特征为该市场空间中的当前工业标准化学消毒剂（例如过氧乙酸）提供了新的、绿色的和有针对性的替代品。该项目解决了将该技术转化为市场所需的知识方面的关键差距。化学消毒剂和机械破碎通常对微生物生物膜的效力有限，尽管进行了清洁，但仍允许潜在病原体在加工环境中持续存在，并导致食源性疾病。此外，由于生物膜群落内存在多种微生物，生物膜化学结构通常是复杂和异质的。该技术通过工程酶解决了这两个差距，这些酶可以降解化学异质性生物膜，并且可以与其他清洁方法协同作用或单独作用，以增强食品加工过程中存在的微生物病原体的去除。特别是，该项目利用了一些领先的行业合作伙伴，如Highland Fresh Technologies，他们将协助设计和模拟实际工艺条件，在这些条件下可以进行应用测试，以确定每次使用的数量和应用方法。此外，将计算扩大规模期间的生产成本，以确定与最终产品相关的关键成本。此外，从事该项目的博士后研究员和研究生将获得技术翻译专业知识，致力于回答确定生产成本，商业计划制定以及直接接触关键行业合作伙伴以设计和执行产品关键测试所需的关键问题。