Chemical and Biochemical Bases for EDTA Biodegradation

EDTA 生物降解的化学和生化基础

• 批准号: 1804699
• 负责人: ChulHee Kang
• 金额: $ 48.89万
• 依托单位: Washington State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1804699&HistoricalAwards=false
• 关键词: Chemical; Biochemical; Bases; EDTA; Biodegradation

This award from the Environmental Chemical Sciences Program in the NSF Division of Chemistry supports a collaborative effort of Professors ChulHee Kang and Luying Xun at Washington State University (WSU) to study the environmental fate of Ethylenediaminetetraacetic acid (EDTA). This compound is being introduced into the environment through extensive use in industrial applications. Currently, EDTA is the most abundant organic pollutant in surface waters around the world, including the US. EDTA forms strong complexes with toxic heavy metals and mobilizes them, which increases exposure of humans. Several EDTA-degrading bacteria exist in nature. However, applying those bacteria in remediation is not practical because they are not efficient enough. Studies of the key enzymes and uptake system for the bacterial EDTA-degradation pathway reveal the factors that limit the biodegradation. This knowledge is used to modify the genes to increase catalytic efficiency and helps generate effective solutions for bioremediation. The research provides the participating students with interdisciplinary training in enzymology, microbiology, and biophysical chemistry, serving as an important bridge among the key STEM units at WSU. In addition, through existing NSF-funded K-12 outreach programs and the BXC Center at WSU, expertise is provided to high school classrooms, the local Palouse Discovery Science Center and local undergraduate institutions. This enhances the training of students of diverse economic, ethnic, and geographic backgrounds.The substrate-specificity, catalytic mechanism and molecular interactions of the transport and binding proteins and enzymes in the EDTA-degradation pathway of Chelativorans sp. BNC1 are investigated. This provides guidance for designing a new recombinant system with enhanced degradation capacity. Those improved EDTA-uptake and metabolizing genes are cloned into phosphite-dependent E. coli to enable the recombinant strain to degrade EDTA in wastewaters. The merit of this approach is not only in its contribution to the study of EDTA biodegradation, but also in its applicability to similar pathways for the degradation of other anthropogenic compounds. The research also addresses the chemical basis of evolutionary adaptations to non-natural chemicals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

NSF化学部环境化学科学计划的这一奖项支持了华盛顿州立大学（WSU）ChulHee Kang和Luying Xun教授的合作努力，以研究乙二胺四乙酸（EDTA）的环境命运。这种化合物通过在工业应用中的广泛使用而被引入环境中。目前，EDTA是包括美国在内的世界各地地表沃茨中含量最丰富的有机污染物。EDTA与有毒重金属形成强络合物并使其流动，这增加了人类的暴露。自然界中存在几种EDTA降解细菌。然而，将这些细菌应用于修复是不切实际的，因为它们不够有效。通过对EDTA降解途径中关键酶和吸收系统的研究，揭示了EDTA生物降解的限制因素。这些知识被用来修改基因，以提高催化效率，并有助于产生有效的生物修复解决方案。该研究为参与的学生提供酶学，微生物学和生物物理化学的跨学科培训，作为WSU关键STEM单位之间的重要桥梁。此外，通过现有的NSF资助的K-12外展计划和WSU的BXC中心，为高中教室、当地的帕卢塞发现科学中心和当地的本科院校提供专业知识。这提高了不同的经济，种族和地理background.The底物特异性，催化机制和运输和结合蛋白质和酶的EDTA降解途径中的分子相互作用的Chelativorans sp. BNC 1的培训。这为设计具有增强的降解能力的新的重组系统提供了指导。这些改进的EDTA吸收和代谢基因被克隆到磷依赖的E。大肠杆菌，使重组菌株降解废水中的EDTA。这种方法的优点不仅在于其对EDTA生物降解研究的贡献，而且还在于其适用于其他人为化合物降解的类似途径。该研究还探讨了非天然化学物质进化适应的化学基础。该奖项反映了NSF的法定使命，并且通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Aminopolycarboxylates by the Periplasmic EDTA- Binding Protein EppA from Chelativorans sp. BNC1

来自 Chelativorans sp. 的周质 EDTA 结合蛋白 EppA 产生的氨基多羧酸盐。

• DOI: 10.3390/ijms21113940
• 发表时间: 2020
• 期刊: International journal of molecular sciences
• 影响因子: 5.6
• 作者: Lewis, Kevin M;Greene, Chelsie;Sattler, Steven A;Buhyun Youn, Buhyun;Xun, Luying;Kang, ChulHee.
• 通讯作者: Kang, ChulHee.

Structural and biochemical characterization of iminodiacetate oxidase from Chelativorans sp. BNC1

Chelativorans sp 亚氨基二乙酸氧化酶的结构和生化特征。

• DOI: 10.1111/mmi.14399
• 发表时间: 2019
• 期刊: Molecular Microbiology
• 影响因子: 3.6
• 作者: Kang, ChulHee;Jun, Se‐Young;Bravo, Abigail G.;Vargas, Erick M.;Liu, Honglei;Lewis, Kevin M.;Xun, Luying
• 通讯作者: Xun, Luying

A Ligand‐Directed Nitrophenol Carbonate for Transient in situ Bioconjugation and Drug Delivery

• DOI: 10.1002/cmdc.202000655
• 发表时间: 2020-10-14
• 期刊: Chemmedchem
• 影响因子: 3.4
• 作者: Burt AJ;Ahmadvand P;Opp LK;Ryan AT;Kang C;Mancini RJ
• 通讯作者: Mancini RJ

Structural studies of Myceliophthora Thermophila Laccase in the presence of deep eutectic solvents

• DOI: 10.1016/j.enzmictec.2021.109890
• 发表时间: 2021-08-21
• 期刊: ENZYME AND MICROBIAL TECHNOLOGY
• 影响因子: 3.4
• 作者: Chan, Jou Chin;Zhang, Bixia;Zhang, Xiao
• 通讯作者: Zhang, Xiao