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Collaborative Research: Decoding and encoding mechanistic relations between structure and function in crack resistance of articular cartilage and cartilage inspired biomaterials.

合作研究：解码和编码关节软骨和软骨启发生物材料的抗裂结构和功能之间的机械关系。

基本信息

批准号：
1807602
负责人：
Itai Cohen
金额：
$ 30万
依托单位：
Cornell University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-07-15 至 2022-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807602&HistoricalAwards=false
关键词：
Collaborative Research Decoding encoding mechanistic

项目摘要

Non-Technical SummaryArticular cartilage is a soft tissue which provides a smooth cushion and distributes mechanical load in joints. As a material, articular cartilage is remarkable. It is only a few millimeters thick, can routinely bear up to ten times one's body weight over 100-200 million loading cycles, and still avoids fracturing. The simultaneous strength, fracture resistance (toughness), and longevity of native articular cartilage remains unmatched in synthetic materials. Such properties are desperately needed for tissue engineering, tissue repair, and even soft robotics applications. The molecular mechanism underlying this exceptional toughness, however, is not well understood. This project will obtain an understanding of the underlying principles and mechanisms that lead to the toughness of articular cartilage, and provide criteria, as we do for cracks in airplane wings, for predicting the probability that initially untreated tears in cartilage will fracture further. The PIs will test the hypothesis that cartilage has such terrific properties due to the fact that it is comprised of two interweaving polymer networks, one which provides mechanical rigidity and one that provides dissipation. Moreover, this double network changes in composition with location in the tissue. These ideas will be tested using numerical simulation and comparison with experimental measurements of the tissue mechanical properties. Using this integrated approach, the PIs will elucidate mechanical structure-function relations underlying fracture toughness of articular cartilage (AC) which will lead to better predictions of cartilage mechanics and failure, and guide the design of new bioinspired materials. The project will provide insights into tissue failure, tissue repair therapies, and design principles for soft robotics. PIs will educate and train a new generation of scientists who understand physics, engineering, and biology, organize workshops aimed at teaching communication skills to graduate students, and promote diversity in STEM workforce. Technical SummaryArticular Cartilage (AC) is a soft tissue that covers the ends of bones to distribute mechanical load in joints. AC contains relatively few cells and its network-like extracellular matrix primarily determines its mechanical response. Its strength, toughness, and crack resistance are extremely high compared to synthetic materials, but the molecular mechanism underlying this exceptional toughness is not well understood. Given the heterogeneous, depth dependent, and multi-component structure and composition of AC, existing continuum descriptions are too coarse-grained to fully describe its fracture mechanics. The PIs will address this challenge by approaching cartilage fracture with a new structure function framework that combines rigidity percolation theory and microscale double-network hydrogel models, together with new confocal elastography experiments that can inform and interface with the model development. Using this integrated approach consisting of multi-scale mathematical modeling and state-of-the art experiments, they will test the hypothesis that the toughness of AC arises because (i) the reinforcing network state is in proximity to a mechanical phase transition allowing tunable mechanical response, and (ii) the tissue is a multi-component heterogeneous composite enabling novel response to stress and blunting of cracks. The project will obtain an understanding of the dependence of cracks on structure and composition of cartilage and similar soft tissues, as well as on loading conditions, and provide insights into tissue failure, and tissue repair therapies. More broadly, this new framework will enable novel and concrete predictions on how these structure, composition, and constitutive mechanical properties can be tuned to resist, and blunt cracks in biomimetic and engineered materials. PIs will educate and train a new generation of scientists who understand physics, engineering, and biology, and promote diversity in STEM workforce. Cohen and Bonassar will develop soft-skills curriculum units for graduate students and postdocs based on a recent science communication workshop held at Cornell by the Alan Alda Center for Communicating Science. Das will mentor minority and 1st generation students via RIT's McNair Program.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

关节软骨是一种软组织，它提供平滑的软垫，并在关节中分配机械载荷。作为一种材料，关节软骨是值得注意的。它只有几毫米厚，在1-2亿次加载循环中，通常可以承受多达10倍的体重，而且仍然可以避免骨折。天然关节软骨的同时强度、抗折性(韧性)和寿命仍然是合成材料无法比拟的。这种特性在组织工程、组织修复、甚至软机器人应用中都是迫切需要的。然而，这种特殊韧性背后的分子机制还不是很清楚。这个项目将获得对导致关节软骨韧性的潜在原理和机制的理解，并提供标准，就像我们对飞机机翼裂缝所做的那样，预测最初未处理的软骨撕裂进一步断裂的可能性。PI将测试软骨具有如此出色特性的假设，因为它由两个相互交织的聚合物网络组成，一个提供机械刚性，另一个提供消散。此外，这种双重网络的组成随组织中的位置而变化。这些想法将通过数值模拟和与组织力学特性的实验测量进行比较来验证。利用这种综合方法，PI将阐明关节软骨(AC)断裂韧性背后的力学结构-功能关系，从而更好地预测软骨的力学和破坏，并指导新的生物启发材料的设计。该项目将提供对组织衰竭、组织修复疗法和软机器人设计原则的见解。PIS将教育和培训了解物理、工程和生物学的新一代科学家，组织旨在向研究生教授沟通技能的研讨会，并促进STEM劳动力的多样性。关节软骨是一种覆盖在骨端的软组织，用于在关节中分配机械载荷。AC含有相对较少的细胞，其网状细胞外基质主要决定其机械反应。与合成材料相比，它的强度、韧性和抗裂性都非常高，但这种特殊韧性背后的分子机制尚不清楚。考虑到AC的非均质、深度相关和多组分的结构和组成，现有的连续介质描述过于粗粒度，无法完全描述其断裂力学。PIS将通过采用新的结构功能框架来解决这一挑战，该框架结合了刚性渗流理论和微型双网络水凝胶模型，以及新的共焦弹性成像实验，可以提供信息并与模型开发相结合。使用这种由多尺度数学建模和最先进的实验组成的综合方法，他们将测试AC韧性产生的假设，这是因为(I)增强网络状态接近允许可调机械响应的机械相变，以及(Ii)组织是能够对应力和钝化裂纹做出新反应的多组分非均质复合材料。该项目将了解裂纹对软骨和类似软组织的结构和组成以及载荷条件的依赖性，并为组织损伤和组织修复疗法提供见解。更广泛地说，这个新的框架将使人们能够对如何调整这些结构、组成和本构机械性能进行新颖而具体的预测，以防止和钝化仿生和工程材料中的裂缝。PIS将教育和培训了解物理、工程和生物学的新一代科学家，并促进STEM劳动力的多样性。科恩和博纳萨将根据艾伦·阿尔达传播科学中心最近在康奈尔举办的科学传播研讨会，为研究生和博士后开发软技能课程单元。DAS将通过RIT的McNair计划指导少数族裔和第一代学生。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Rigidity and fracture of biopolymer double networks

生物聚合物双网络的刚性和断裂

DOI：
10.1039/d1sm00802a
发表时间：
2022
期刊：
Soft Matter
影响因子：
3.4
作者：
Lwin, Pancy;Sindermann, Andrew;Sutter, Leo;Wyse Jackson, Thomas;Bonassar, Lawrence;Cohen, Itai;Das, Moumita
通讯作者：
Das, Moumita

The influence of chondrocyte source on the manufacturing reproducibility of human tissue engineered cartilage