UNS: Nanotopographical Memory Modulates Stem Cell Fate

UNS：纳米地形记忆调节干细胞命运

• 批准号: 1807734
• 负责人: Yong Yang
• 金额: $ 24.51万
• 依托单位: University of North Texas
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2020-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807734&HistoricalAwards=false
• 关键词: UNS; Nanotopographical; Memory; Modulates; Stem

1511759Yang, Yong Conventional cell culture methods using flat, stiff plastic surfaces do not recapitulate characteristics (e.g., stiffness and nanotopography) of the extracellular matrix (ECM) with which cells interact in vivo. Therefore, cell behavior on such surfaces significantly deviate from their in vivo counterparts. There is a pressing need to incorporate the ECM characteristics into stem cell culture technologies. The goal of this research is to advance next-generation cell culture technologies by investigating nanotopographical memory effects of stem cells.ECM/substrate nanotopography and stiffness critically influence numerous developmental, physiological and pathological processes in vivo, and have a profound influence on cell behavior and stem cell fate decision in vitro. It is hypothesized that stem cells can retain nanotopographical information from past culture environments and the nanotopographical memory can influence future fate decision of stem cells. The objective of the proposed research is to test this hypothesis and to delineate the underlying mechanism of nanotopographical memory effects. Three research objectives using human mesenchymal stem cells (hMSCs) as model cells are proposed to study the nanotopographical memory effects: (1) Regulate Yes-associated protein (YAP) intracellular localization by using nanotopography, (2) Validate nanotopographical memory effects, and (3) Delineate the underlying mechanism of nanotopographical memory effects. This research will advance the understanding of cellular mechanotransduction and stem cell plasticity during developmental and pathological processes. It will call attention to unintended nanotopographical memory effects during in vitro culture, which may affect stem cell function and differentiation. This research will contribute to the development of next-generation stem cell culture technologies, and provide insight into the design of new biomaterials and the cell-substrate interfaces of implants and medical devices for regenerative medicine. In addition, a collaborative, cross-disciplinary, learn-through-research system will be established to provide the basic infrastructure to promote nanobiotechnology education in West Virginia.This award by the Biotechnology and Biochemical Engineering Program of CBET is co-funded by the Biomaterials Program of the Division of Materials Research.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

1511759yang，使用平坦的僵硬塑料表面的传统细胞培养方法不会概括细胞外基质（ECM）的特征（例如，刚度和纳米造影），细胞与体内相互作用。 因此，这种表面上的细胞行为显着偏离了它们的体内对应物。迫切需要将ECM特征纳入干细胞培养技术中。这项研究的目的是通过研究干细胞的纳米体记忆效应来推进下一代细胞培养技术。ECM/底物纳米摄影和僵硬严重影响体内的许多发育，生理和病理过程，并对体外的细胞行为和干细胞命运决策产生深远的影响。 假设干细胞可以从过去的培养环境中保留纳米形象信息，纳米形态记忆会影响干细胞的未来命运决策。拟议的研究的目的是检验这一假设，并描述纳米形态记忆效应的潜在机制。 Three research objectives using human mesenchymal stem cells (hMSCs) as model cells are proposed to study the nanotopographical memory effects: (1) Regulate Yes-associated protein (YAP) intracellular localization by using nanotopography, (2) Validate nanotopographical memory effects, and (3) Delineate the underlying mechanism of nanotopographical memory effects.这项研究将在发育和病理过程中提高对细胞机械转导和干细胞可塑性的理解。它将引起人们对体外培养期间意外纳米形态记忆效应的关注，这可能会影响干细胞功能和分化。这项研究将有助于开发下一代干细胞培养技术，并深入了解新生物材料的设计以及用于再生医学的植入物和医疗设备的细胞底物界面。此外，还将建立一个协作，跨学科的学习研究系统，以提供基本的基础架构来促进西弗吉尼亚州的纳米生物学技术教育。该奖项由CBET的生物技术和生物化学工程计划颁发。CBET的生物化学工程计划由材料研究的材料研究及其奖项。使用基金会的智力优点和更广泛的影响评估标准进行评估。