Elucidating the Molecular and Hierarchical Structure of Spider Silk

阐明蜘蛛丝的分子和层次结构

• 批准号: 1809645
• 负责人: Jeffery Yarger
• 金额: $ 39.39万
• 依托单位: Arizona State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1809645&HistoricalAwards=false
• 关键词: Elucidating; Molecular; Hierarchical; Structure; Spider

Non-Technical SummarySpiders are the masters of silk: a typical web-spinning spider produces at least six different silks, each synthesized and spun by different silk glands and spinnerets located on its abdomen. The ultimate goal of this research is to be able to mimic this process and materials in the laboratory. Silk fibers produced by spiders are protein-based and currently there is not a good molecular understanding of the structure responsible for the "high-performance" mechanical properties of spider silks. Understanding the molecular structure, dynamics and the hierarchical organizations in spider silk proteins is critical to the ultimate goal of producing materials in the lab with properties comparable to natural spider silk fibers. This research group plans to explore the molecular structure, protein-protein interactions, and hierarchical structures formed in silk proteins (the silks that produce dragline and web-building for spiders). This research will establish relationships between hierarchical structure and mechanical function in a variety of natural silk proteins from several different spiders, including orb-weaving, cob-weaving and jumping spiders. This is a critical component to realize silk biomimicry.Technical SummaryA single orb-weaving spider produces up to 6 different types of biopolymer fibers that are commonly called silk. These silk fibers produced by spiders are protein-based biopolymers and span a large range of physical and mechanical properties. Currently, there is not a comprehensive molecular and hierarchical understanding of the protein structures responsible for the "high-performance" mechanical properties of spider silks. The critical component of spider silk fibers is the fact that they are almost completely protein based, and clearly contain a complex set of secondary protein structures, nanocrystalline domains and hierarchical organizations of these molecular level structures. Understanding the molecular structure and the hierarchical organizations in spider silk proteins is critical to the ultimate goal of producing a protein-based material with properties similar to natural spider silk fibers. This research group plans to explore the molecular structure, protein-protein interactions, and hierarchical structures formed in major and minor ampullate silk proteins (dragline and web-building) with an ultimate goal to establish relationships between hierarchical structure and mechanical function in a variety of natural silk proteins from several evolutionarily different spiders, including orb-weaving (Araneus), cob-weaving (Latrodectus) and jumping (Peucetia) spiders.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

蜘蛛是吐丝的大师：一只典型的结网蜘蛛至少能产生六种不同的丝，每一种丝都是由位于腹部的不同丝腺和小囊合成和纺出的。这项研究的最终目标是能够在实验室中模拟这种过程和材料。 蜘蛛产生的丝纤维是基于蛋白质的，目前还没有一个很好的分子结构的理解负责蜘蛛丝的“高性能”机械性能。 了解蜘蛛丝蛋白的分子结构，动力学和层次组织对于在实验室中生产具有与天然蜘蛛丝纤维相当的性能的材料的最终目标至关重要。该研究小组计划探索丝蛋白（为蜘蛛产生拖丝和结网的丝）中形成的分子结构、蛋白质-蛋白质相互作用和层次结构。本研究将建立等级结构和机械功能之间的关系，在各种天然丝蛋白从几个不同的蜘蛛，包括圆织，织蛛和跳蛛。 这是实现丝仿生的关键组成部分。技术概述一只圆织蜘蛛可以产生多达6种不同类型的生物聚合物纤维，通常称为丝。 蜘蛛产生的这些丝纤维是基于蛋白质的生物聚合物，具有广泛的物理和机械性能。目前，还没有一个全面的分子和层次结构的蛋白质结构负责的“高性能”的机械性能的蜘蛛丝的理解。 蜘蛛丝纤维的关键组成部分是它们几乎完全基于蛋白质的事实，并且显然包含一组复杂的二级蛋白质结构，纳米晶结构域和这些分子水平结构的分级组织。 了解蜘蛛丝蛋白的分子结构和层次组织对于生产具有与天然蜘蛛丝纤维相似特性的蛋白质材料的最终目标至关重要。本研究小组计划探索大壶腹丝蛋白和小壶腹丝蛋白的分子结构、蛋白质-蛋白质相互作用和等级结构（拖丝和织网），最终目标是在来自几种进化上不同的蜘蛛的各种天然丝蛋白中建立等级结构和机械功能之间的关系，包括圆织蜘蛛（Araneus），该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Relation of Ionic Conductivity to Solvent Rotation Times in Dinitrile Plastic Crystal Solvents

二腈塑料晶体溶剂中离子电导率与溶剂旋转时间的关系

• DOI: 10.1149/1945-7111/ab847b
• 发表时间: 2020
• 期刊: Journal of The Electrochemical Society
• 影响因子: 3.9
• 作者: Davidowski, Stephen;Young-Gonzales, Amanda R.;Richert, Ranko;Yarger, Jeff;Angell, C. Austen
• 通讯作者: Angell, C. Austen

Thermodynamic interference with bile acid demicelleization reduces systemic entry and injury during cholestasis

• DOI: 10.1038/s41598-020-65451-w
• 发表时间: 2020-05-21
• 期刊: SCIENTIFIC REPORTS
• 影响因子: 4.6
• 作者: de Oliveira, Cristiane;Khatua, Biswajit;Singh, Vijay P.
• 通讯作者: Singh, Vijay P.

Hierarchical spidroin micellar nanoparticles as the fundamental precursors of spider silks

• DOI: 10.1073/pnas.1810203115
• 发表时间: 2018-11-06
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Parent, Lucas R.;Onofrei, David;Holland, Gregory P.
• 通讯作者: Holland, Gregory P.

Extended range X-ray pair distribution functions

• DOI: 10.1016/j.nima.2019.163318
• 发表时间: 2020-03-01
• 期刊: NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT
• 影响因子: 1.4
• 作者: Benmore, C. J.;Alderman, O. L. G.;Weber, J. K. R.
• 通讯作者: Weber, J. K. R.

Hard x-ray methods for studying the structure of amorphous thin films and bulk glassy oxides

用于研究非晶薄膜和块状玻璃氧化物结构的硬 X 射线方法

• DOI: 10.1088/1361-648x/abe352
• 发表时间: 2021
• 期刊: Journal of Physics: Condensed Matter
• 作者: Benmore, C J;González, G B;Alderman, O L;Wilke, S K;Yarger, J L;Leinenweber, K;Weber, J K
• 通讯作者: Weber, J K