Collaborative Research: Spatiotemporal Regulation of the Ethylene Signaling Network and Rapid Adaptive Responses in Plants

合作研究：乙烯信号网络的时空调控和植物的快速适应性反应

• 批准号: 1817304
• 负责人: Brad Binder
• 金额: $ 21.35万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1817304&HistoricalAwards=false
• 关键词: Collaborative; Research; Spatiotemporal; Regulation; Ethylene

The goal of this project is to better understand how the gaseous plant hormone ethylene, commonly produced when plants are under stress, acts to regulate adaptation to various environmental inputs such as drought, chilling, salinity, flooding and nutrient deficiency. All of these stresses commonly affect the growth and reproduction of plants, ultimately threatening global food security. Plants undergo adaptive responses to these stresses by modifying physiological processes in which plant hormones such as ethylene play a pivotal role. This project will not only uncover the role of ethylene in plant adaptive response, potentially contributing to improving sustainable agriculture and food security, it will also provide research training for one postdoctoral scientist and two graduate students. The project leaders will also mentor undergraduate students through summer internship opportunities as well as other undergraduate research activities. In addition, the project will provide outreach activities to the general public, focusing on local high school students by allowing them to experience personal laboratory exercises and research presentations.Rapid adaptation of plants to their surroundings is a critical trait for the survival of the plant and is regulated by how fast plants alter their growth patterns by modulating cellular dynamics. However, little is known about the fundamental aspects of adaptive responses such as response kinetics and the correlation of these kinetics with cellular events. The goal of this research is to fill this knowledge gap by utilizing ethylene-regulated dark-grown seedlings as a model system. The plant hormone ethylene plays a key role in plant adaptation, in addition to its well-known function in plant growth and development. Furthermore, dark-grown seedlings provide an excellent model system to study plant adaptation, as ethylene-induced changes are easy to trigger and to assay and the signaling pathway has been well characterized. Moreover, several studies indicate that the reversible growth kinetics of dark-grown seedlings in response to ethylene is closely regulated by the spatiotemporal regulation of ethylene signaling molecules. This provides a unique opportunity to study the correlation between adaptive response kinetics with the altered cellular dynamics directing this change. In order to accomplish these research goals, the investigators will determine the movement kinetics of the central ethylene signaling molecules in response to ethylene using cell biology approaches and its relation to the growth kinetics of dark-grown seedlings using high-resolution, time-lapse imaging analysis. In addition, they will test the hypothesis that a RAF-like protein kinase called Constitutive Triple Response 1 (CTR1), a negative regulator of ethylene signaling, plays a key role in a rapid resetting of the ethylene response, leading to faster growth recovery of dark-grown seedlings after ethylene treatment.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目的目的是更好地理解气态植物激素乙烯如何在植物处于压力下通常会产生，以调节对各种环境投入的适应性，例如干旱，寒冷，盐度，盐度，洪水和营养不足。所有这些压力通常会影响植物的生长和繁殖，最终威胁着全球粮食安全。植物通过修改植物激素（如乙烯起关键作用）的生理过程来对这些胁迫进行适应性反应。该项目不仅将揭示乙烯在植物适应性反应中的作用，这可能有助于改善可持续农业和粮食安全，还将为一位博士后科学家和两名研究生提供研究培训。项目领导者还将通过暑期实习机会以及其他本科研究活动来指导本科生。此外，该项目将通过允许他们体验个人实验室练习和研究演讲来向公众提供外展活动，重点关注当地的高中生。将植物改编成周围的植物是植物生存的关键特征，并且受植物如何通过调节细胞动力学来改变其生长模式。然而，关于自适应反应的基本方面，例如反应动力学以及这些动力学与细胞事件的相关性知之甚少。这项研究的目的是通过利用乙烯调节的深色生长幼苗作为模型系统来填补这一知识差距。除了众所周知的植物生长和发育功能外，植物激素乙烯在植物适应中起着关键作用。此外，由于乙烯引起的变化易于触发和测定，深色生长的幼苗为研究植物的适应提供了出色的模型系统，并且信号传导途径已得到很好的特征。此外，几项研究表明，响应乙烯的黑暗幼苗的可逆生长动力学受到乙烯信号分子的时空调节的严格调节。这提供了一个独特的机会，可以研究自适应反应动力学与导致这种变化的细胞动力学改变之间的相关性。为了实现这些研究目标，研究人员将使用细胞生物学方法来确定中央乙烯信号分子的运动动力学对乙烯的响应及其与深色生长幼苗的生长动力学的关系，并使用高分辨率，时光化成像分析。此外，他们还将检验以下假说：埃乙烯信号传导的负调节剂称为本型三重反应1（CTR1），在快速重置乙烯反应中起着关键作用，从而在快速重置乙烯响应中起着关键作用，从而使乙烯奖的基础范围通过nsf的构建范围来表现出来。和更广泛的影响审查标准。