Ethylene Cross-Kingdom Signaling In Beneficial Plant-Microbe Associations

有益植物-微生物关联中的乙烯跨界信号传导

• 批准号: 1855066
• 负责人: Brad Binder
• 金额: $ 68.32万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1855066&HistoricalAwards=false
• 关键词: Ethylene; Cross; Kingdom; Signaling; Beneficial

With an increasing human population, future food security largely depends on sustainable agricultural practices that ensure elevated crop productivity with minimal environmental impact. Optimizing the microbial community that associates with plants is a possible strategy that is being pursued. Ethylene gas is produced by plants and functions as a plant hormone where it is known to affect many processes. Preliminary findings demonstrate that ethylene receptors are present in bacteria as well as plants. Many of these bacteria associate with plants and with none being pathogenic, it follows that ethylene produced by plants may function to regulate beneficial plant-microbe interactions. Results from this research on the role of ethylene in plant-microbe interactions will provide information needed to improve associations between plants and beneficial microbes to enhance food production and security. The research will also increase educational infrastructure by broadening opportunities for undergraduate and graduate students at the UT-Knoxville to engage in modern biological research. The project will test the hypothesis that ethylene produced by plants mediates the establishment of some beneficial bacteria in the rhizosphere via regulating biofilm formation and attachment of the bacteria to the plants. Preliminary data demonstrate that the genomes of several non-pathogenic and non-symbiotic, but potentially beneficial, proteobacteria encode homologs to plant ethylene receptors, with the Azospirillum brasilense homolog binding ethylene in vitro. Further evidence suggests that ethylene sensing by the bacteria modulates biofilm formation. The molecular mechanism of ethylene signaling and associated responses in bacteria will be characterized using a combination of genetic and biochemical approaches in genetically amenable bacterial models. The role of ethylene signaling in modulating root surface colonization by beneficial bacteria will be studied using plant colonization assays combined with genetic or chemical manipulation of ethylene signaling in model and crop plants. The possibility that ethylene signaling between plant and beneficial bacteria functions to modulate plant immune responses will also be tested. Results from the proposed research will generate novel models for plant-bacteria signaling that could yield innovative strategies to engineer beneficial plant-microbe associations in the rhizosphere.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

随着人口的增加，未来的粮食安全在很大程度上取决于可持续的农业实践，这些实践确保农作物生产力提高，环境影响最小。优化与植物相关的微生物社区是一种可能正在采取的策略。乙烯气体由植物产生，并作为植物激素的作用，在该植物激素中会影响许多过程。初步发现表明，细菌和植物中存在乙烯受体。这些细菌中的许多与植物相关，没有致病性，因此，植物产生的乙烯可能起作用以调节有益的植物 - 微生物相互作用。这项关于乙烯在植物菌相互作用中作用的研究的结果将提供改善植物与有益微生物之间关联以增强粮食生产和安全性所需的信息。这项研究还将通过扩大UT-Knoxville的本科和研究生的机会来从事现代生物学研究的机会，从而增加教育基础设施。 该项目将检验以下假设：植物产生的乙烯通过调节生物膜形成并将细菌的附着附着在根际中介导某些有益细菌。初步数据表明，几种非致病性和非生物生物和非亲身性但潜在有益的蛋白质细菌的基因组编码同源物，以植入乙烯受体，并在体外与氮杂螺螺的巴西硫硫糖菌同源物结合乙烯。进一步的证据表明，细菌传感调节生物膜的形成。乙烯信号传导和细菌中相关反应的分子机制将通过遗传和生化方法的结合来表征遗传性的细菌模型。将使用植物定植测定法与模型和农作物植物中乙烯信号的遗传或化学操纵相结合，研究乙烯信号传导在调节根部表面定植中的作用。还将测试植物和有益细菌功能之间的乙烯信号传导调节植物免疫反应的可能性。拟议的研究的结果将为植物 - 细菌信号传导生成新型模型，这些模型可以产生创新的策略，从而在根际中为有益的植物 - 微生物关联来设计，该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子和更广泛影响的评估来通过评估来获得支持的。