PFI-TT: Automated Manufacturing of Blood Vessels

PFI-TT：血管的自动化制造

• 批准号: 1827422
• 负责人: Jeffrey Morgan
• 金额: $ 20万
• 依托单位: Brown University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2020-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1827422&HistoricalAwards=false
• 关键词: PFI; TT; Automated; Manufacturing; Blood

The broader impact/commercial potential of this PFI project is a new and innovative method for the automated manufacture of living blood vessels. This research will yield new scientific as well as engineering knowledge into the automation processes to manufacture a new class of living products. Such fundamental knowledge will help usher in the next era of high-value bio-manufacturing and is needed to sustain the competitive advantage of our manufacturing economy. As a product, manufactured living bloods vessels have significant commercial potential in at least two areas. Manufactured blood vessels will be a less expensive alternative that replaces the use of animals in the testing of medical devices. Manufactured blood vessels will be used to treat and repair cardiovascular disease.The proposed project will design, build and test a new fully automatic system for the manufacture of small living blood vessels to be used for reducing the use of animals in the testing of medical devices and to be ultimately used to treat patients with heart disease. To develop this automated manufacturing system, the project will add human cells to a specially designed mold to form living building parts in the shape of round donuts. A robot will be programmed to retrieve these donut-shaped living building parts one-by-one and drop them into a specially designed funnel where the stack of donuts will form a long tube or living blood vessel. The built blood vessels will be housed in a bioreactor tank that controls the temperature and recirculates the broth needed to provide the nutrients that nourish and mature the living blood vessels until they are ready for use. This novel automated process will manufacture living blood vessels for research testing and for clinical application.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该 PFI 项目更广泛的影响/商业潜力是一种自动制造活血管的创新方法。这项研究将为自动化过程带来新的科学和工程知识，以制造新型生活产品。这些基础知识将有助于开创高价值生物制造的下一个时代，并且是维持我们制造业经济的竞争优势所必需的。作为一种产品，人造活血管至少在两个领域具有巨大的商业潜力。人造血管将是一种更便宜的替代品，可以取代在医疗设备测试中使用动物。人造血管将用于治疗和修复心血管疾病。拟议项目将设计、建造和测试一种新型全自动系统，用于制造小型活体血管，以减少医疗设备测试中动物的使用，并最终用于治疗心脏病患者。为了开发这种自动化制造系统，该项目将把人类细胞添加到专门设计的模具中，以形成圆形甜甜圈形状的活体建筑部件。 机器人将被编程为逐一检索这些甜甜圈形状的活体建筑部件，并将它们放入专门设计的漏斗中，其中一堆甜甜圈将形成一根长管或活血管。构建的血管将被安置在一个生物反应器罐中，该生物反应器罐控制温度并再循环所需的肉汤，以提供滋养和成熟活血管的营养，直到它们可供使用。这种新颖的自动化流程将制造用于研究测试和临床应用的活体血管。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Toward Automated Additive Manufacturing of Living Bio-Tubes Using Ring-Shaped Building Units

• DOI: 10.1177/2472630320920896
• 发表时间: 2020-05-26
• 期刊: SLAS TECHNOLOGY
• 影响因子: 2.7
• 作者: Manning, Kali L.;Feder, Jacob;Morgan, Jeffrey R.
• 通讯作者: Morgan, Jeffrey R.