MRI: Acquisition of a Fluorescence Microscopy System for Research and Teaching at Kettering University

MRI：凯特林大学购买荧光显微镜系统用于研究和教学

• 批准号: 1828246
• 负责人: Ronald Kumon
• 金额: $ 25.13万
• 依托单位: Kettering University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828246&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluorescence; Microscopy; System

This award is to purchase an advanced fluorescence microscopy system for Kettering University. The system will enable high-resolution, three-dimensional imaging of cells on a microscopic scale. These capabilities allow molecules and nanoparticles that have potential for enhancing disease treatments to be tracked relative to various parts of the cell. The system will support interdisciplinary research in studying cellular processes with the goal of improving treatment of cancer and other diseases. The system will provide excellent training opportunities for students to prepare them for graduate school or employment in scientific fields after graduation. The system will also enable science-oriented outreach to women and other underrepresented minorities via pre-college programs and summer research experiences for undergraduates. Furthermore, the instrument will be used to provide professional development for teachers and enhance science education of under-served students in Flint and surrounding areas via field trips to the university and Internet-enabled remote microscopy demonstrations.This instrument will include an inverted microscope with epifluorescence components and light source, an electron-multiplier CCD camera, and a confocal laser scanning system, all of which are critical for the study of the localization and transport of molecules and nanoparticles. The first project will study the mechanisms of ultrasound-mediated delivery of fluorescent agents to cells in the presence of microbubbles, thereby potentially enabling better non-invasive spatiotemporal control of drug delivery. The second project will study the symmetry-breaking mechanisms of eukaryotic cells during movement with fluorescent dyes, thereby contributing to an improved understanding of metastasis. The third project will study the uptake and distribution of fluorescent magnetic nanoparticles in cancerous cells and spheroids with magnetic and ultrasonic methods. These magnetic nanoparticles can be used for noninvasive hyperthermic cancer treatment via induction heating. The fourth project will study the biotoxicity of fluorescent silver nanoparticles on yeast as a model for treating infectious fungi. The fifth project will test fluorescent gold nanoclusters as a means to image beta-amyloid aggregates and study the process of beta-amyloid plaque formation towards a better understanding of Alzheimer's disease. The final project will develop fluorescent quantum dots and characterize their ability to act as nanothermometers in the intra- and extracellular environment during hyperthermia.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项是为凯特林大学购买先进的荧光显微镜系统。该系统将能够在微观尺度上对细胞进行高分辨率的三维成像。这些能力允许相对于细胞的各个部分跟踪具有增强疾病治疗潜力的分子和纳米颗粒。该系统将支持研究细胞过程的跨学科研究，以改善癌症和其他疾病的治疗。该系统将为学生提供极好的培训机会，为他们毕业后进入研究生院或在科学领域就业做好准备。该系统还将通过大学预科课程和本科生暑期研究经验，向妇女和其他代表性不足的少数民族提供以科学为导向的外联服务。此外，该仪器还将用于为教师提供专业发展，并通过到大学进行实地考察和通过互联网进行远程显微镜演示，加强弗林特及周边地区服务不足学生的科学教育，该仪器将包括一个带有落射荧光组件和光源的倒置显微镜、一个电子倍增器CCD相机和一个共焦激光扫描系统，所有这些对于分子和纳米颗粒的定位和运输的研究都是至关重要的。第一个项目将研究在存在微泡的情况下超声介导的荧光剂向细胞输送的机制，从而有可能实现对药物输送的更好的非侵入性时空控制。第二个项目将研究荧光染料运动过程中真核细胞的破坏机制，从而有助于提高对转移的理解。第三个项目将利用磁性和超声方法研究荧光磁性纳米颗粒在癌细胞和球体中的摄取和分布。这些磁性纳米颗粒可用于通过感应加热进行非侵入性热疗癌症。第四个项目将研究荧光银纳米粒子对酵母的生物毒性，作为治疗感染性真菌的模型。第五个项目将测试荧光金纳米团簇作为一种手段来成像β-淀粉样蛋白聚集体，并研究β-淀粉样蛋白斑块形成的过程，以更好地了解阿尔茨海默病。最后的项目将开发荧光量子点，并表征其在热疗过程中在细胞内和细胞外环境中充当纳米温度计的能力。该奖项反映了NSF的法定使命，并被认为值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估来支持。