Berry College MRI: Aquisition of a Flex Real-Time PCR System for Undergraduate Research and Teaching

贝里学院 MRI：为本科生研究和教学购置 Flex 实时 PCR 系统

• 批准号: 1828540
• 负责人: Angela Poole
• 金额: $ 9.96万
• 依托单位: Berry College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828540&HistoricalAwards=false
• 关键词: Berry; College; MRI; Aquisition; Flex

An award is made to Berry College to increase the research productivity of faculty and enhance undergraduate research and teaching through the acquisition of an Applied Biosystems QuantStudio 7 Flex Real-Time PCR system. Over the three-year grant period, the QuantStudio 7 will enable faculty to engage an estimated 77 undergraduate research assistants and 312 undergraduates in coursework, teaching them modern molecular techniques and comprehensively preparing them for graduate school and careers in science. This instrument will create opportunities for undertaking new laboratory exercises and engaging in research within the classroom in a diverse set of courses, including Molecular Biology, Developmental Biology, Genetics of Domesticated Animals, and Chromatin, Epigenetics, and Gene Expression. The QuantStudio 7 will also facilitate partnerships between Berry and other institutions, including Dalton State College (an HSI) and Colorado State University. In partnership with Armuchee High School (serving economically diverse students) and the Georgia Governor's Honors Program (a summer program held on the Berry campus that serves Georgia's top science students from diverse backgrounds), Berry will provide an estimated 348 high school students with early exposure to molecular research instrumentation and methods, which will help build their confidence in their ability to pursue STEM majors and careers. Given the impact of Berry's activities using the QuantStudio 7, as well as the research and training planned by shared users in the Northwest Georgia region, this instrument will play a role in producing the qualified STEM workers the state needs to meet projected STEM employment growth. It will also further Berry's strategic goal to graduate students who are "life ready" prepared to be agents of improvement through the integration of rigorous academic learning with meaningful applied learning experiences and a heart for service. The QuantStudio 7 will foster and facilitate the OneHealth approach to research, i.e., transdisciplinary collaborations between physicians, veterinarians, epidemiologists, ecologists, climatologists, medical researchers, and numerous other specialties, as dictated by the specific problem being studied. In the immediate future, the QuantStudio 7 will bring together the project's four PIs a diverse group that includes three early-career investigators and other faculty who collectively are studying coral reef ecosystem health, wildlife and domesticated animal health, and parasites related to human health. With access to a modern real-time PCR system, the PIs' research will advance understanding of a broad spectrum of molecular mechanisms. Specifically, the PIs will use the instrument to study potential immune genes and endocrine disruption in cnidarians to better understand how these organisms respond to a changing environment; develop an ideal method for cryopreserving eggs, sperm, and embryos of goat and deer species for agricultural purposes and wildlife conservation; and determine how genetic variants affect early pregnancy and thus facilitate genetic selection programs to improve cow fertility. The PIs will disseminate their research findings through submission of papers to peer-reviewed journals and presentations at national and international meetings. Overall, the QuantStudio 7's robust capabilities will advance future investigations in interconnections between human, animal, and environmental health.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

授予贝瑞学院通过收购应用生物系统QuantStudio 7 Flex实时PCR系统来提高教师的研究生产力并加强本科生的研究和教学。 在三年的资助期内，QuantStudio 7将使教师能够聘请大约77名本科生研究助理和312名本科生进行课程学习，教授他们现代分子技术，并为他们进入研究生院和从事科学职业做好全面准备。该仪器将创造机会进行新的实验室练习，并在课堂上从事研究，在一套不同的课程，包括分子生物学，发育生物学，驯化动物的遗传学，染色质，表观遗传学和基因表达。QuantStudio 7还将促进Berry与其他机构之间的合作，包括道尔顿州立大学（HSI）和科罗拉多州立大学。 与Armuchee High School合作（服务于经济多样化的学生）和格鲁吉亚州长荣誉计划（在贝里校园举行的暑期课程，为来自不同背景的格鲁吉亚顶尖科学学生提供服务），贝里将为估计348名高中生提供早期接触分子研究仪器和方法，这将有助于建立他们对自己追求STEM专业和职业的能力的信心。鉴于Berry使用QuantStudio 7的活动的影响，以及格鲁吉亚西北地区共享用户计划的研究和培训，该工具将在培养该州所需的合格STEM工作者方面发挥作用，以满足预计的STEM就业增长。它还将进一步贝瑞的战略目标，研究生谁是“生活准备”准备通过严格的学术学习与有意义的应用学习经验和服务的心脏相结合的改进代理人。QuantStudio 7将促进和促进OneHealth的研究方法，即，医生、兽医、流行病学家、生态学家、气候学家、医学研究人员和许多其他专业之间的跨学科合作，这取决于所研究的具体问题。在不久的将来，QuantStudio 7将汇集该项目的四名PI，这是一个多元化的团队，其中包括三名早期职业调查人员和其他教师，他们共同研究珊瑚礁生态系统健康，野生动物和驯养动物健康以及与人类健康相关的寄生虫。 通过使用现代实时PCR系统，PI的研究将促进对广泛分子机制的理解。具体来说，研究人员将利用该仪器研究刺胞动物的潜在免疫基因和内分泌干扰，以更好地了解这些生物如何应对不断变化的环境;开发一种理想的方法，用于冷冻保存山羊和鹿的卵子、精子和胚胎，用于农业目的和野生动物保护;并确定遗传变异如何影响早期妊娠，从而促进遗传选择计划，以提高奶牛的生育能力。研究所将通过向同行评审的期刊提交论文以及在国家和国际会议上发表演讲来传播其研究成果。 总体而言，QuantStudio 7的强大功能将推动未来对人类、动物和环境健康之间相互关系的研究。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Effects of a blend of Saccharomyces cerevisiae-based direct-fed microbial and fermentation products on plasma carbonyl-metabolome and fecal bacterial community of beef steers

• DOI: 10.1186/s40104-019-0419-5
• 发表时间: 2020-02-17
• 期刊: JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
• 影响因子: 7
• 作者: Adeyemi, James A.;Peters, Sunday O.;Ogunade, Ibukun M.
• 通讯作者: Ogunade, Ibukun M.

Differential expression of Exaiptasia pallida GIMAP genes upon induction of apoptosis and autophagy suggests a potential role in cnidarian symbiosis and disease

• DOI: 10.1242/jeb.229906
• 发表时间: 2020-11-01
• 期刊: JOURNAL OF EXPERIMENTAL BIOLOGY
• 影响因子: 2.8
• 作者: Bailey, Grace F.;Coelho, Jenny C.;Poole, Angela Z.
• 通讯作者: Poole, Angela Z.