MRI: Acquisition of a Hybrid Quadrupole Orbitrap LC/MS System to Facilitate Trace Quantitation and Complex Mixture Analyses at the University of Alaska Anchorage

MRI：阿拉斯加大学安克雷奇分校购买混合四极 Orbitrap LC/MS 系统以促进痕量定量和复杂混合物分析

• 批准号: 2019123
• 负责人: Patrick Tomco
• 金额: $ 45万
• 依托单位: University of Alaska Anchorage Campus
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2019123&HistoricalAwards=false
• 关键词: MRI; Acquisition; Hybrid; Quadrupole; Orbitrap

This award is supported by the Major Research Instrumentation, the Chemistry Research Instrumentation programs and the Established Program to Stimulate Competitive Research (EPSCoR). The University of Alaska-Anchorage is acquiring a high-performance benchtop quadrupole system that combines a quadrupole, an ion routing multipole and an Orbitrap analyzer and a liquid to support Professor Patrick Tomco, Jason Burkhead, Aaron Dotson, Colin McGill, and other colleagues. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. An instrument with a liquid chromatograph can separate mixtures of compounds before they reach the mass spectrometer. In a quadrupole mass spectrometer, there are four cylindrical rods parallel to each other, that serve as the mass analyzer (the component of the instrument responsible for selecting sample ions based on their mass-to-charge ratio (m/z)). Ions are separated in a quadrupole based on the stability of their trajectories in oscillating electric fields that are applied to the rods. In this instrument, this capability is coupled to an ion trap mass analyzer consisting of an outer barrel-like electrode and a coaxial inner spindle-like electrode that traps ions in an orbital motion around the spindle. The image from the trapped ions is detected and converted to a mass spectrum using the Fourier transform of the frequency signal. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used in outreach activities involving the University of Alaska-Fairbanks (UAF) and the University of Alaska-Southeast (UAS) as well as agencies such as the Alaska Department of Fish & Game and the Alaska State Crime Lab and collaborators from the University of New Orleans.The award of this mass spectrometer is aimed at enhancing research and education at all levels across the University of Alaska system. It especially impacts understanding the fate of the insecticide rotenone isoflavone in the changing climate of southcentral Alaska. The instrumentation is also used for exploring environmental oil spill damage mitigation in the Artic and Sub-artic and for the study of metabolomics. In addition, it contributes to the analysis of the impact of climate change on plant-herbivore dynamics in the Artic and Boreal ecoregions, and the study of medicinal properties of Alaskan botanicals. The mass spectrometer is also used to understand essential amino acid metabolism in the microbiomes of hibernatic Artic ground squirrels using 15-nitrogen-labeled nitrogen sources.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具，化学研究工具计划和启发竞争性研究的既定计划（EPSCOR）的支持。阿拉斯加大学建立的大学正在收购一个高性能的台式四极杆系统，该系统结合了四倍长，一个离子路由多台，一个Orbitrap分析仪和液体，以支持Patrick Tomco教授，Jason Burkhead，Jason Burkhead，Aaron Dotson，Aaron Dotson，Aaron Dotson，Colin McGill等。通常，质谱法（MS）是用于识别和表征复杂样品中少量化学物种的关键分析方法之一。具有液相色谱仪的仪器可以在到达质谱仪之前将其分离化合物的混合物。在四极质量光谱仪中，有四个平行于彼此的圆柱杆，它们可以用作质量分析仪（仪器的组成部分，负责根据其质量到电荷比（M/z）选择样品离子）。基于其轨迹在适用于杆上的振荡电场中的轨迹的稳定性，将离子分离为四极杆。在该仪器中，该能力耦合到由外部桶状电极和同轴内纺锤体样电极组成的离子陷阱质量分析仪，该电极将离子捕获在纺锤体周围的轨道运动中。检测到来自捕获的离子的图像使用频率信号的傅立叶变换转换为质谱。此次收购加强了大学和地区地区的研究基础设施。该乐器通过让多样化的学生使用这种现代分析技术来扩大参与。它还用于涉及阿拉斯加大学 - 福尔班克斯大学（UAF）和阿拉斯加 - 东风（UAS）（UAS）以及阿拉斯加鱼类与游戏系等机构以及新奥尔良大学的合作者等机构。它特别影响了了解阿拉斯加西南部气候变化的杀虫剂烤面包酮异黄酮的命运。该仪器还用于探索环境油的溢出损害减轻损害，并在次级机构和代谢组学研究中进行研究。此外，它有助于分析气候变化对植物 - 植物动力学在北方生态区域中的影响，以及对阿拉斯加植物学药物的药用特性的研究。该质谱仪还用于了解使用15氮标记的氮来源的冬眠交通型松鼠中必需的氨基酸代谢。这项奖项反映了NSF的法定任务，并被认为是通过基金会的智力功能和广泛的影响来评估CRETERIA的评估，认为NSF的法定任务值得通过评估。

项目成果

Photochemical formation of water-soluble oxyPAHs, naphthenic acids, and other hydrocarbon oxidation products from Cook Inlet, Alaska crude oil and diesel in simulated seawater spills

模拟海水泄漏中库克湾、阿拉斯加原油和柴油中水溶性多环芳烃、环烷酸和其他碳氢化合物氧化产物的光化学形成

• DOI: 10.1039/d2va00325b
• 发表时间: 2023
• 期刊: Environmental Science: Advances
• 作者: Harsha, Maxwell L.;Redman, Zachary C.;Wesolowski, Josh;Podgorski, David C.;Tomco, Patrick L.
• 通讯作者: Tomco, Patrick L.

Reactive Oxygen Species and Chromophoric Dissolved Organic Matter Drive the Aquatic Photochemical Pathways and Photoproducts of 6PPD-quinone under Simulated High-Latitude Conditions

• DOI: 10.1021/acs.est.3c05742
• 发表时间: 2023-11-30
• 期刊: ENVIRONMENTAL SCIENCE & TECHNOLOGY
• 影响因子: 11.4
• 作者: Redman，Zachary C.;Begley，Jessica L.;Tomco，Patrick L.
• 通讯作者: Tomco，Patrick L.