Collaborative Research: Inferring Cellular Lysis and Regeneration of Organic Matter by Marine Viruses

合作研究:推断海洋病毒对有机物的细胞裂解和再生

基本信息

  • 批准号:
    1829641
  • 负责人:
  • 金额:
    $ 59.84万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Continuing Grant
  • 财政年份:
    2018
  • 资助国家:
    美国
  • 起止时间:
    2018-10-01 至 2023-09-30
  • 项目状态:
    已结题

项目摘要

Viral infections of marine microbes can transform the fate of microbial populations that fuel global ocean biogeochemical cycles. For example, viral infections of microbes lead to the release of carbon and nutrients back into the environment. This regeneration of carbon and nutrients stimulates the activity of other microbes and diverts carbon and nutrients from larger organisms in marine food webs. Because virus-microbe infections are relatively specific, it is critical to identify those pairs of viruses and microbes that may disproportionally contribute to the turnover of carbon and nutrients in the ocean. This project will develop quantitative approaches and tools to quantify which viruses infect which microbes and to use these data to quantify how viral infections of microbes collectively shape nutrient and carbon cycles in the North Atlantic Ocean. The project will analyze virus-microbe interactions in mesocosms at the Bigelow Laboratory for Ocean Sciences in mid-coast Maine and during open ocean expeditions to the Bermuda Atlantic Time-Series Study (BATS) site. An interdisciplinary team will leverage recent advances in molecular biology, computational biology, and mathematical modeling to identify virus-host partners and their impact on the movement of elements through marine systems. This project will support three graduate students, six undergraduate students and one postdoctoral researcher in an interdisciplinary context. Research advances will be translated into reproducible software methods to be disseminated via the community cyberinfrastructure platform iVirus, with additional training materials presented as part of a viral methods and informatics workshop held at The Ohio State University. The translation of discoveries to the public will be furthered by the involvement of journalism undergraduate students at the University of Tennessee-Knoxville. This project builds upon advances in the molecular toolkit of viromics to develop an integrated approach to characterize lineage-specific rates of infection, lysis, and nutrient release induced by marine viruses in open ocean ecosystems. It will combine theory, in vitro experiments, and in situ sampling to (i) extend a robust inference method for estimating virus-microbe cross-infection networks from time-series data; (ii) establish and characterize in-vitro protocols for inferring cross-infectivity in complex communities using culture-independent methods; (iii) estimate lineage-specific rates of lysis and regeneration of nutrients in marine systems, including applications to coastal and open ocean ecosystems. Project aims focus on quantifying the extent to which virus-induced lysis and regeneration of carbon and nutrients is heterogeneously distributed across microbial populations. To do so, the project will incorporate time series measurements of abundance information (via metagenomes) and activity information (via metatranscriptomes). In so doing, it will advance efforts to understand community-scale interactions rather than those amongst a single virus-host pair. Theoretical methods and in vitro protocols will directly infer lineage-specific infection, lysis, and nutrient release rates in coastal- and open-ocean ecosystems in the North Atlantic Ocean. Results will be used to identify key links that disproportionately influence bulk nutrient release. A novel PCR-based approach will augment and validate the core inference approach. Overall, the project aims to enhance our understanding of how viruses contribute to marine ecosystem function.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
海洋微生物的病毒感染可以改变微生物种群的命运,为全球海洋生物地球化学循环提供燃料。例如,微生物的病毒感染导致碳和营养物质释放回环境中。这种碳和营养物质的再生刺激了其他微生物的活动,并将碳和营养物质从海洋食物网中的大型生物转移。由于病毒-微生物感染是相对特异的,因此确定那些可能对海洋中碳和营养物质的周转有贡献的病毒和微生物对至关重要。该项目将开发定量方法和工具来量化哪些病毒感染哪些微生物,并使用这些数据来量化微生物的病毒感染如何共同塑造北大西洋的营养和碳循环。 该项目将在缅因州中部海岸的毕格罗海洋科学实验室和在百慕大大西洋时间序列研究(BATS)地点的公海考察期间分析中型生态系统中的病毒-微生物相互作用。一个跨学科的团队将利用分子生物学,计算生物学和数学建模的最新进展,以确定病毒宿主合作伙伴及其对海洋系统中元素运动的影响。该项目将在跨学科背景下支持三名研究生,六名本科生和一名博士后研究员。 研究进展将转化为可复制的软件方法,通过社区网络基础设施平台iVirus传播,并提供额外的培训材料,作为在俄亥俄州州立大学举行的病毒方法和信息学研讨会的一部分。 田纳西-诺克斯维尔大学新闻系本科生的参与将进一步推动将发现转化为公众。该项目建立在病毒组学的分子工具包的进步,开发一种综合的方法来表征特定的谱系感染率,裂解,并在开放的海洋生态系统中引起的海洋病毒的营养释放。它将结合联合收割机理论、体外实验和原位采样,以(i)扩展一种稳健的推断方法,用于从时间序列数据中估计病毒-微生物交叉感染网络;(ii)建立和表征体外协议,用于使用非培养方法推断复杂社区的交叉感染性; ㈢估计海洋系统中营养物溶解和再生的特定谱系速率,包括应用于沿海和公海生态系统。项目的重点是量化病毒诱导的裂解和碳和营养物质再生在微生物种群中的非均匀分布程度。为此,该项目将纳入丰度信息(通过宏基因组)和活动信息(通过元转录组)的时间序列测量。通过这样做,它将推动人们了解社区规模的相互作用,而不是单个病毒-宿主对之间的相互作用。理论方法和体外协议将直接推断谱系特异性感染,溶解和营养释放率在沿海和开放的海洋生态系统在北大西洋。 结果将被用来确定不成比例地影响散装养分释放的关键环节。 一种新的基于PCR的方法将增强和验证核心推理方法。总体而言,该项目旨在提高我们对病毒如何促进海洋生态系统功能的理解。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Cryopreservation of Paramecium bursaria Chlorella Virus-1 during an active infection cycle of its host
  • DOI:
    10.1371/journal.pone.0211755
  • 发表时间:
    2019-01
  • 期刊:
  • 影响因子:
    3.7
  • 作者:
    Samantha R. Coy;A. Alsante;J. V. Van Etten;S. Wilhelm
  • 通讯作者:
    Samantha R. Coy;A. Alsante;J. V. Van Etten;S. Wilhelm
Trace metal contents of autotrophic flagellates from contrasting open‐ocean ecosystems
  • DOI:
    10.1002/lol2.10258
  • 发表时间:
    2022-05
  • 期刊:
  • 影响因子:
    7.8
  • 作者:
    L. E. Sofen;Olga A. Antipova;M. Ellwood;N. Gilbert;Gary R. Lecleir;M. Lohan;C. Mahaffey;Elizabeth L. Mann;D. Ohnemus;S. Wilhelm;B. Twining
  • 通讯作者:
    L. E. Sofen;Olga A. Antipova;M. Ellwood;N. Gilbert;Gary R. Lecleir;M. Lohan;C. Mahaffey;Elizabeth L. Mann;D. Ohnemus;S. Wilhelm;B. Twining
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Steven Wilhelm其他文献

Studies of the Di/tripeptide Transporter in Saccharomyces cerevisiae : The N-terminal Cytoplasmic Domain of Ptr2p is Involved in Post-Translational Regulation
酿酒酵母二肽/三肽转运蛋白的研究:Ptr2p N 端胞质结构域参与翻译后调节
  • DOI:
  • 发表时间:
    2008
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Steven Clinton Minkin;Jeffrey M. Becker;Daniel Roberts;Timothy Sparer;Barry Bruce;Steven Wilhelm
  • 通讯作者:
    Steven Wilhelm

Steven Wilhelm的其他文献

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{{ truncateString('Steven Wilhelm', 18)}}的其他基金

REU Site: Microbial community interactions and functions
REU 网站:微生物群落相互作用和功能
  • 批准号:
    2050743
  • 财政年份:
    2021
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Continuing Grant
EDGE CT: Genetic tools to study giant viruses
EDGE CT:研究巨型病毒的遗传工具
  • 批准号:
    1922958
  • 财政年份:
    2019
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
REU Site: Microbial community interactions and functions
REU 网站:微生物群落相互作用和功能
  • 批准号:
    1659599
  • 财政年份:
    2017
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
Collaborative Research: An Integrated Approach to Understanding the Function of the Potent Hepatotoxin Microcystin in the Growth & Ecology of Microcystis
合作研究:了解强效肝毒素微囊藻毒素在生长中功能的综合方法
  • 批准号:
    1451528
  • 财政年份:
    2015
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Continuing Grant
Dimensions: Collaborative Research: Anthropogenic nutrient input drives genetic, functional and taxonomic biodiversity in hypereutrophic Lake Taihu, China
维度:合作研究:人为养分输入驱动中国超富营养化太湖的遗传、功能和分类生物多样性
  • 批准号:
    1240870
  • 财政年份:
    2013
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
REU Site: Microbial community interactions and functions
REU 网站:微生物群落相互作用和功能
  • 批准号:
    1156644
  • 财政年份:
    2012
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
Collaborative Research: Characterizing the Constraints on Virus Infection of Cyanobacteria
合作研究:表征蓝藻病毒感染的限制
  • 批准号:
    0851113
  • 财政年份:
    2009
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
What Makes Microcystis Bloom? Dissecting the Physiological Ecology of a Toxic Cyanobacterium with Community Level Proteomics
是什么让微囊藻开花?
  • 批准号:
    0841918
  • 财政年份:
    2009
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
Collaborative Proposal: Decoding Virus Leviathans
合作提案:解码病毒利维坦
  • 批准号:
    0949120
  • 财政年份:
    2009
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Standard Grant
Collaborative Research: Evaluating Nutrient Reductions to Control Cyanobacteria and Ensure Large Lake Sustainability: Lake Taihu (China) as a Model for North American Systems
合作研究:评估控制蓝藻的营养减少并确保大型湖泊的可持续性:太湖(中国)作为北美系统的模型
  • 批准号:
    0826838
  • 财政年份:
    2008
  • 资助金额:
    $ 59.84万
  • 项目类别:
    Continuing Grant

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合作研究:CISE-MSI:用于大规模推断物联网恶意行为的主动和被动互联网测量
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