What Makes Microcystis Bloom? Dissecting the Physiological Ecology of a Toxic Cyanobacterium with Community Level Proteomics

是什么让微囊藻开花？

• 批准号: 0841918
• 负责人: Steven Wilhelm
• 金额: $ 45.87万
• 依托单位: University of Tennessee Knoxville
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-03-01 至 2014-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0841918&HistoricalAwards=false
• 关键词: What; Makes; Microcystis; Bloom; Dissecting

Although the toxic cyanobacterium Microcystis continues to be the focus of studies around the globe, scientists remain unable to definitively answer the question 'what makes Microcystis bloom?' Using genomic and proteomic information, researchers at the University of Tennessee and Oak Ridge National Laboratory will collect 'snapshots' of the biochemical pathways that are simultaneously active in Microcystis cells before, during, and after bloom conditions. By comparing these pathways with lab cultures grown under controlled conditions, researchers will be able to for the first time tease apart the complicated interactions between the environment and Microcystis population growth. To accomplish this goal the researchers will integrate new data sets of recently completed genomic sequences of Microcystis with state-of-the-art tools that analyze whole cell and community protein expression. The resulting information will provide an accurate map of the physiological status of cells under different growth conditions as well as during bloom formation, climax and termination. The work will also provide information on how the environment influences cellular pathways, an important step in understanding the connections between ecological organism response and cellular biochemical processes.The project will facilitate outreach and diversification at an EPSCoR Institution (The University of Tennessee) and provide training for undergraduate and graduate students. The PIs will continue to engage underrepresented groups in the research and will disseminate results to the public through science-focused journalism interns.

虽然有毒的蓝藻微囊藻仍然是地球仪研究的焦点，但科学家们仍然无法明确回答“是什么使微囊藻开花？”利用基因组和蛋白质组学信息，田纳西大学和橡树岭国家实验室的研究人员将收集微囊藻细胞在水华之前、期间和之后同时活跃的生化途径的“快照”。通过将这些途径与在受控条件下生长的实验室培养物进行比较，研究人员将能够首次梳理出环境与微囊藻种群增长之间复杂的相互作用。为了实现这一目标，研究人员将把最近完成的微囊藻基因组序列的新数据集与分析全细胞和群落蛋白表达的最先进工具相结合。所得到的信息将提供在不同生长条件下以及在水华形成、高潮和终止期间细胞生理状态的准确地图。这项工作还将提供有关环境如何影响细胞通路的信息，这是理解生态有机体反应和细胞生化过程之间联系的重要一步。该项目将促进EPSCoR机构（田纳西大学）的推广和多样化，并为本科生和研究生提供培训。PI将继续让代表性不足的群体参与研究，并通过以科学为重点的新闻实习生向公众传播结果。