RII Track-4: Using the Cryopreservation and Microfluidic Technologies to Engineer Bottlenose Dolphin Ovarian Function for Testing Marine Contaminants' Female Reproductive Toxicity
RII Track-4:利用低温保存和微流体技术改造宽吻海豚卵巢功能,以测试海洋污染物的雌性生殖毒性
基本信息
- 批准号:1832910
- 负责人:
- 金额:$ 21.44万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2018
- 资助国家:美国
- 起止时间:2018-10-01 至 2020-09-30
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Nontechnical description of the projectThe bottlenose dolphin is the most common marine mammal along the U.S. coastal states and is designated as a federally protected species under the Marine Mammals Protection Act (MMPA). As one of the apex predators and by having extensive lipid-rich blubber layers, the bottlenose dolphin is more susceptible to the bioaccumulation of lipid soluble marine contaminants. Many of these contaminants are endocrine disrupting chemicals (EDCs) such as polychlorinated biphenyls (PCBs) and phthalates, that can adversely impact the female reproductive functions of marine mammals. Studies related to investigating the effect of marine contaminants on female bottlenose dolphin reproduction are dependent on recording whether a dolphin can be pregnant and produce a viable calf. However, it is difficult to monitor the specific impact of EDC's on the ovary and its related functions. The central objective of this research proposal is to use the cryopreservation and 3D microfluidic technologies to engineer the bottlenose dolphin ovarian function in vitro. The proposed research will allow the establishment of a high-throughput ovarian toxicity screening platform to determine the reproductive toxicity of marine contaminants on female bottlenose dolphins as well as other marine mammals.Technical description of the projectThe ovary is the primary female reproductive organ and contains follicles at various developmental stages. The follicle primarily functions to secrete sex steroid hormones and to support its enclosed germ cell oocyte for maturation, ovulation, and fertilization. The goal of the proposed research is to visit the host site at Oregon Health and Science University to learn ovarian tissue cryopreservation and the in vitro growth of follicles from large mammalian species. The first research objective of research one is to cryopreserve mouse ovarian follicles through vitrification for establishing a long-term follicle storage platform. The PI will culture follicles in vitro and examine the follicle reproductive outcomes to investigate the effect of vitrification on follicle and oocyte health and also determine the vitrification-induced cell injury. The second research objective is to cryopreserve bottlenose dolphin ovarian follicles and use microfluidic technology to engineer dolphin ovarian function in vitro. We will collect and cryopreserve ovaries from fresh-dead, stranded bottlenose dolphins, use the microfluidic platform to support a long-term dolphin follicle development and maturation, and validate this system by testing known ovarian toxic chemicals. The proposed work will allow the development of a high-throughput platform to study the female reproductive toxicity of marine contaminants on female bottlenose dolphins and enhance PI and his team's research ability in female reproductive toxicology in marine animals. The broader impact of this study will result in a platform that can be used for cryopreserving ovarian tissues and for other cell/tissue types. Microfluidic culture will provide a novel method to three-dimensionally grow mammalian cells/tissues in vitro.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
项目非技术描述宽吻海豚是美国沿海各州最常见的海洋哺乳动物,根据《海洋哺乳动物保护法》(MMPA)被指定为联邦保护物种。作为顶端捕食者之一,宽吻海豚具有广泛的富含脂肪的鲸脂层,更容易被脂类溶解的海洋污染物生物积累。其中许多污染物是内分泌干扰物,如多氯联苯和邻苯二甲酸盐,会对海洋哺乳动物的雌性生殖功能产生不利影响。与调查海洋污染物对雌性宽吻海豚繁殖的影响有关的研究,取决于记录一只海豚是否能够怀孕并产下成活的幼崽。然而,很难监测EDC对卵巢及其相关功能的具体影响。本研究方案的中心目标是利用冷冻保存和三维微流控技术来体外工程宽吻海豚的卵巢功能。这项拟议的研究将建立一个高通量的卵巢毒性筛选平台,以确定海洋污染物对雌性宽吻海豚和其他海洋哺乳动物的生殖毒性。项目技术说明卵巢是主要的雌性生殖器官,包含不同发育阶段的卵泡。卵泡的主要功能是分泌性类固醇激素,并支持其封闭的生殖细胞卵母细胞成熟、排卵和受精。这项拟议的研究的目标是访问俄勒冈健康与科学大学的宿主站点,以了解卵巢组织的冷冻保存和大型哺乳动物卵泡的体外生长。研究一的第一个研究目标是通过玻璃化冷冻保存小鼠卵巢卵泡,以建立一个长期的卵泡储存平台。PI将体外培养卵泡,并检测卵泡的生殖结果,以了解玻璃化冷冻对卵泡和卵母细胞健康的影响,并确定玻璃化冷冻引起的细胞损伤。第二个研究目标是冷冻保存宽吻海豚的卵泡,并利用微流控技术在体外工程海豚的卵巢功能。我们将收集和冷冻新鲜死亡的宽吻海豚的卵巢,使用微流体平台支持海豚卵泡的长期发育和成熟,并通过测试已知的卵巢有毒化学物质来验证该系统。拟议的工作将允许开发一个高通量平台,研究海洋污染物对雌性宽吻海豚的雌性生殖毒性,并增强Pi及其团队在海洋动物雌性生殖毒理学方面的研究能力。这项研究的更广泛影响将导致一个可用于冷冻保存卵巢组织和其他细胞/组织类型的平台。微流控培养将提供一种在体外三维培养哺乳动物细胞/组织的新方法。该奖项反映了NSF的法定使命,并通过使用基金会的智力优势和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Shuo Xiao其他文献
Bayesian analysis of physiologically based toxicokinetic (PBTK) modeling for pentachlorophenol exposure in pregnant women
孕妇五氯苯酚暴露的生理学毒代动力学 (PBTK) 模型的贝叶斯分析
- DOI:
10.1016/j.tiv.2024.105853 - 发表时间:
2024 - 期刊:
- 影响因子:0
- 作者:
Chunfeng Wu;Yajiao Tan;Xiaoyi Wei;Xun Li;Sifei Sun;Bing Lyu;Zhen Shen;Xiao Wei;Shuo Xiao;Yuanyuan Ruan;Jun Yu;Gengsheng He;Weiwei Zheng;Jingguang Li - 通讯作者:
Jingguang Li
Improved One-Dimensional Convolutional Neural Networks for Human Motion Recognition
用于人体运动识别的改进一维卷积神经网络
- DOI:
10.1109/bibm49941.2020.9313296 - 发表时间:
2020 - 期刊:
- 影响因子:0
- 作者:
Shengzhi Wang;Shuo Xiao;Zhenzhen Huang;Zhiou Xu;W. Chen - 通讯作者:
W. Chen
Post-fire mechanical properties of stainless steel cables
- DOI:
10.1016/j.jcsr.2020.106177 - 发表时间:
2020-09-01 - 期刊:
- 影响因子:
- 作者:
Guojun Sun;Shuo Xiao;Yuan Yang;Xiaohui Li;Martin Mensinger - 通讯作者:
Martin Mensinger
Experimental study on anti-slip performance of galvanized cable-clamped joint at elevated temperature
高温下镀锌索夹接头防滑性能的试验研究
- DOI:
10.1038/s41598-025-89571-3 - 发表时间:
2025-02-17 - 期刊:
- 影响因子:3.900
- 作者:
Guojun Sun;Yujia Lin;Jinzhi Wu;Shuo Xiao;Yu Xue - 通讯作者:
Yu Xue
Highly efficient Cu-based catalysts via hydrotalcite-like precursors for CO2 hydrogenation to methanol
通过类水滑石前体实现二氧化碳加氢制甲醇的高效铜基催化剂
- DOI:
10.1016/j.cattod.2016.02.004 - 发表时间:
2017-03 - 期刊:
- 影响因子:5.3
- 作者:
Shuo Xiao;Yanfei Zhang;Peng Gao;Liangshu Zhong;Xiaopeng Li;Zhongzheng Zhang;Hui Wang;Wei Wei;Yuhan Sun - 通讯作者:
Yuhan Sun
Shuo Xiao的其他文献
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