RII-Track 4: Elucidating the Structure and Interactions of Complex Carbohydrates in Fungal and Plant Cell Walls via Dynamic Nuclear Polarization Solid-State NMR

RII-Track 4：通过动态核极化固态 NMR 阐明真菌和植物细胞壁中复杂碳水化合物的结构和相互作用

• 批准号: 1833040
• 负责人: Tuo Wang
• 金额: $ 17.96万
• 依托单位: Louisiana State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1833040&HistoricalAwards=false
• 关键词: RII; Track; Elucidating; Structure; Interactions

Non-Technical description The complex carbohydrate is a fundamental building unit of cell walls in a variety of organisms such as plants, fungi, bacteria and algae, which serve as a central source for bioenergy and biomaterials as well as a potential target of medical therapies. These polysaccharides are often polymorphic and disordered in their native state, however, due to technical limitations their functional structure remains under-investigated. This EPSCoR project will strengthen the collaborative efforts between Louisiana State University and National High Magnetic Field Laboratory (NHMFL), with the aim of developing a novel method to address several fundamental scientific questions that are related to polysaccharides. Specifically, the proposed research will advance understanding of the tendency and mechanism for carbohydrates to interact with other biomolecules as well as the building principles and conserved modules of cell walls. These molecular insights will guide the design and engineering of carbohydrate-rich composites to fulfill the diverse needs of the nation for better biofuel, material, and biomedicine. This collaborative project also allows scientists and students to gain frequent exposure to state-of-art magnet facilities, training at NHMFL and introduce a new technique that will build up the research capacity of Louisiana. Technical descriptionThis project aims to expand knowledge of carbohydrate research from soluble or crystalline molecules in purified components to the highly polymorphic, insoluble and disordered glycans in native cells. This goal will be achieved by integrating Dynamic Nuclear Polarization (DNP) technique and solid-state NMR methods to characterize the structure and the assembly of complex carbohydrates with minimal perturbation. By combing the sensitivity enhancement from DNP and the resolution improvement from spectral editing, the PI will be able to conduct site-specific investigations of polysaccharides in a complex whole-cell system. Structural characterizations will start from 13C,15N-labeled tissues and then progressively extend to unlabeled materials. This approach, once established, will alleviate the difficulty and expense that is typically associated with isotope-labeling, thus overcoming a long-standing barrier to large-scale structural elucidation. To enable rapid access to the results, a database will be established to document the NMR observables and structural restraints of polysaccharides as well as the diverse architecture of cell walls and other biomaterials. The proposed method and database has the potential to facilitate the modification of carbohydrate-rich composites to serve national demand for better bioenergy and materials. This collaborative project also provides a faculty and a postdoctoral fellow with the access to high-field NMR and DNP magnets at National High Magnetic Field Laboratory (NHMFL) and grants invaluable learning experiences through extensive interactions with well-established experts, thus stimulating the career development of next-generation scientists.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

非技术描述复合碳水化合物是多种生物体如植物、真菌、细菌和藻类中细胞壁的基本构建单元，其作为生物能源和生物材料的中心来源以及医学治疗的潜在靶点。这些多糖在其天然状态下通常是多晶型和无序的，然而，由于技术限制，它们的功能结构仍然研究不足。这个EPSCoR项目将加强路易斯安那州立大学和国家高磁场实验室（NHMFL）之间的合作，目的是开发一种新的方法来解决与多糖有关的几个基本科学问题。具体而言，拟议的研究将促进对碳水化合物与其他生物分子相互作用的趋势和机制以及细胞壁的构建原理和保守模块的理解。这些分子见解将指导富含碳水化合物的复合材料的设计和工程，以满足国家对更好的生物燃料，材料和生物医学的各种需求。这个合作项目还使科学家和学生能够经常接触到最先进的磁铁设施，在NHMFL接受培训，并引入一种新技术，以提高路易斯安那州的研究能力。本项目旨在将碳水化合物研究的知识从纯化组分中的可溶性或结晶分子扩展到天然细胞中的高度多态性、不溶性和无序的聚糖。这一目标将通过整合动态核极化（DNP）技术和固态NMR方法来表征复杂碳水化合物的结构和组装，以最小的扰动来实现。通过结合DNP的灵敏度增强和光谱编辑的分辨率提高，PI将能够在复杂的全细胞系统中对多糖进行位点特异性研究。结构表征将从13C，15N标记的组织开始，然后逐步扩展到未标记的材料。这种方法一旦建立，将减轻通常与同位素标记相关的困难和费用，从而克服大规模结构解析的长期障碍。为了能够快速获得结果，将建立一个数据库，以记录多糖的NMR可观测值和结构限制以及细胞壁和其他生物材料的不同结构。所提出的方法和数据库有可能促进富含碳水化合物的复合材料的改性，以满足国家对更好的生物能源和材料的需求。这个合作项目还提供了教师和博士后研究员在国家高磁场实验室（NHMFL）获得高场NMR和DNP磁体，并通过与知名专家的广泛互动，赠款宝贵的学习经验，从而刺激下一代的职业发展，该奖项反映了NSF的法定使命，并被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准。

项目成果

Lignin-polysaccharide interactions in plant secondary cell walls revealed by solid-state NMR

• DOI: 10.1038/s41467-018-08252-0
• 发表时间: 2019-01-21
• 期刊: NATURE COMMUNICATIONS
• 影响因子: 16.6
• 作者: Kang, Xue;Kirui, Alex;Wang, Tuo
• 通讯作者: Wang, Tuo

Integrated solid-state NMR and molecular dynamics modeling determines membrane insertion of human β-defensin analog

• DOI: 10.1038/s42003-019-0653-6
• 发表时间: 2019-11-01
• 期刊: COMMUNICATIONS BIOLOGY
• 影响因子: 5.9
• 作者: Kang, Xue;Elson, Christopher;Wang, Tuo
• 通讯作者: Wang, Tuo

Atomic resolution of cotton cellulose structure enabled by dynamic nuclear polarization solid-state NMR

• DOI: 10.1007/s10570-018-2095-6
• 发表时间: 2019-01-01
• 期刊: CELLULOSE
• 影响因子: 5.7
• 作者: Kirui, Alex;Ling, Zhe;Wang, Tuo
• 通讯作者: Wang, Tuo

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

• DOI: 10.3791/59152
• 发表时间: 2019-02-01
• 期刊: JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
• 影响因子: 1.2
• 作者: Kirui, Alex;Widanage, Malitha C. Dickwella;Wang, Tuo
• 通讯作者: Wang, Tuo

Effects of ball milling on the structure of cotton cellulose

• DOI: 10.1007/s10570-018-02230-x
• 发表时间: 2019-01-01
• 期刊: CELLULOSE
• 影响因子: 5.7
• 作者: Ling, Zhe;Wang, Tuo;French, Alfred D.
• 通讯作者: French, Alfred D.