Cloning and mapping of the Brassica napus orthologs of the AtPirin gene associated with seed germination and seedling vigour, and generation of molecular markers to break the linkage with yellow seed colour in oilseed rape

黄色种子对于油菜（Brassica napus）育种是特别感兴趣的，因为它们与导致降低的膳食纤维含量的较薄的种皮相关联。这大大提高了榨油后菜籽粕的饲料和蛋白质质量。然而，黄籽表型也与负面的农艺性状相关，包括发芽率降低和抑制幼苗发育和活力。然而，油菜中的关联程度存在遗传变异。在拟南芥中，基因AtPirin1对种子萌发和早期幼苗发育具有类似的作用，并且是B。已经发现该基因的欧洲油菜同源物（BnPirin）在黄籽油菜中强烈过表达。基于BnPirin与B中的黄籽色主效部分显性基因紧密连锁的假设。本项目的目的是鉴定和定位B中AtPirin基因的所有直系同源物。并研究它们在不同黄籽油菜品系中的表达。通过将BnPirin图谱位置与发芽率、幼苗发育、活力和相关植物生长激素（特别是脱落酸和乙烯）的QTL进行比较，目的是开发该基因转录活性拷贝的分子标记，可用于标记辅助选择（MAS）黄籽油菜品系，发芽率和幼苗发育（活力）没有连锁阻力。

Yellow coloured seeds are of particular interest for oilseed rape (Brassica napus) breeding because of their association with a thinner seed coat resulting in reduced dietary fibre content. This considerably improves the feed and protein quality of rapeseed meal after oil extraction. However, the yellow-seeded phenotype is also associated with negative agronomic characters including a reduced germination rate and inhibition of seedling development and vigour. However, genetic variation exists with regard to the degree of association in rapeseed. In Arabidopsis, the gene AtPirin1 has an analogous effect on seed germination and early seedling development, and a B. napus homolog of this gene (BnPirin) has been found to be strongly over-expressed in yellow-seeded oilseed rape. Based on the hypothesis that BnPirin is closely linked to the major partially dominant gene for yellow seed colour in B. napus, the aim of this project is to identify and map all orthologs of the AtPirin gene in B. napus and study their expression in different yellow-seeded rapeseed lines. By comparing the BnPirin map locations with QTL for germination rate, seedling development, vigour and relevant plant growth hormones (particularly abscisic acid and ethylene), the intention is to develop molecular markers for transcriptionally active copies of the gene that can be used for a marker-assisted selection (MAS) of yellow-seeded rapeseed lines with no linkage drag for germination rate and seedling development (vigour).