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Strategies of low temperature survival in Arabidopsis thaliana

拟南芥低温生存策略

基本信息

批准号：
236129611
负责人：
Professor Dr. Arnd Göran Heyer
金额：
--
依托单位：
Abteilung Pflanzen-Biotechnologie
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2019-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/236129611?language=en
关键词：
Strategies low temperature survival Arabidopsis

项目摘要

In the first year working on the project "Strategies of low temperature survival in Arabidopsis thaliana", we could prove multiple sub-cellular re-allocations of metabolites during cold acclimation by combining the methods of non-aqueous fractionation of compartments and quantitative metabolic profiling using GC-MS. Investigations on the metabolic mutants pgm and spsa1 that are defective in starch metabolism and strongly reduced in sucrose turnover, respectively, revealed strong evidence that a plastidial association of raffinose, cytosolic prolin and possibly the transfer of reducing equivalents and / or intermediates of phototranspiration between plastids and mitochondria are relevant for freezing tolerance. In a second phase of the project we want to expand these analyses to natural accessions of Arabidopsis that represent a broad spectrum of freezing tolerance. This shall allow drawing a "metabolic map" of cold acclimation with sub-cellular resolution. In particular, we want to follow up the evidence we obtained for exchange of aspartate / malate and glutamate / 2-oxo-glutarate for the transfer of reducing equivalents as well as ornithine / citrulline for the transfer of intermediates of photorespiration between mitochondria and plastids. n parallel, thermographic analysis of freezing events shall reveal whether Arabidopsis in general or specific natural accessions are able to tolerate ice formation in intracellular spaces, and whether certain metabolic patterns correlate with this ability. Modification of the content of relevant metabolites shall be used to alter freezing tolerance.

在“拟南芥低温生存策略”项目的第一年，我们通过结合非水分离隔间和GC-MS定量代谢分析的方法，证明了在冷驯化过程中代谢物的多个亚细胞重新分配。对代谢突变体pgm和spsa1的研究表明，棉子糖和细胞质脯氨酸的质体结合，以及在质体和线粒体之间光蒸腾作用的还原等价物和/或中间体的转移与抗冻性有关。在该项目的第二阶段，我们希望将这些分析扩展到具有广泛抗冻性的拟南芥天然品种。这样就可以绘制出亚细胞分辨率的冷驯化“代谢图”。特别是，我们想要跟进我们获得的证据，证明天冬氨酸/苹果酸和谷氨酸/ 2-氧-戊二酸交换用于还原等价物的转移，以及鸟氨酸/瓜氨酸用于线粒体和质体之间光呼吸中间体的转移。与此同时，冷冻事件的热成像分析将揭示拟南芥是否能够在细胞内空间耐受冰的形成，以及某些代谢模式是否与这种能力相关。改变相关代谢物应使用含量，改变抗冻性。