Subcellular analysis of metabolic network dynamics under elevated CO2

点击翻译按钮获取中文摘要

Photosynthesis and primary metabolism of higher plants are tightly regulated. Upon environmental changes, plants need to immediately adjust their metabolism in order to prevent chronic tissue damage and cell death. The study of metabolic regulation in changing environments is complicated by the high degree of compartmentalization of plant cells. Based on previous findings, our project aims to quantify dynamics of subcellular metabolic networks under elevated CO2 (eCO2), applying a combination of subcellular fractionation, omics analysis and mathematical modeling. Further, the objective of this project is to quantitatively assess the role of three enzymes with differential subcellular localization to assess their role under such growth conditions. Previously, we found evidence for a role of HEXOKINASE 1 (HXK1) in photorespiration and a potential regulatory interaction with HYDROXYPYRUVATE REDUCTASE 1 (HPR1) and the mitochondrial transporter A BOUT DE SOUFFLE (BOU). Thus, in our project, we aim to decipher the role of HXK1 for subcellular kinetics of primary metabolism under eCO2 to reveal how HXK1-deficiency affects photorespiratory pathways. We will combine subcellular metabolomics with proteomics and transcriptomics to identify subcellular consequences of the hpr1 and bou mutations, their relevance under eCO2 and possible interactions with HXK1. Finally, we will develop and apply a genome-scale data integration platform for analysis of subcellular metabolism. We will combine transcriptome and proteome dynamics for context-specific network reconstruction and for the analysis of subcellular metabolite dynamics. This will reveal similarities and discrepancies between networks reconstructed from transcriptomics and proteomics data and it will show how photorespiratory metabolism interacts with and depends on HXK1.