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CAREER: Multiplexed Paper-Based Biosensor Enabled by Interfacial Interactions

职业：通过界面相互作用实现多重纸基生物传感器

基本信息

批准号：
1846846
负责人：
Charles Mace
金额：
$ 50万
依托单位：
Tufts University
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2025-05-31
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1846846&HistoricalAwards=false
关键词：
CAREER Multiplexed Paper Based Biosensor

项目摘要

The use of modern instrumentation for biochemical analysis is impractical in limited-resource settings where electrical power and trained operators may not be available and where analytical solutions must be low-cost, portable, and operationally simple. Fundamentally new approaches that are designed specifically to address the challenges of limited-resource settings are needed. Utilizing paper as a substrate to develop biosensors is attractive because the resources required to produce them are minimal and the materials are ubiquitous and inexpensive. This project will create new device designs that can enable paper-based biosensing. Hydrophilic (wettable) channels will be patterned in paper using hydrophobic (non-wettable) barriers in order to control the transport of sample fluids by wicking. In addition to devising a new strategy for bioanalysis in limited-resource settings, the broader impacts of this research program include the creation of educational opportunities for middle school students (hands-on workshops), high school students (device design challenges and summer internships), and science teachers (classroom kit development).Due to their low cost and ease of use, paper-based microfluidic devices have gained attention as a platform to develop point-of-use bioassays. Independent of the advances in assay development, the paper-based devices themselves, in spite of their vast potential to facilitate bioanalysis, have received surprisingly little attention. This project aims to build an integrated research and education program centered on developing novel paper-based microfluidic devices that can facilitate biosensing based on chemical and biochemical reactions that occur at the interfaces formed between converging liquid fronts. Devices exploiting this approach will facilitate multiplexed biosensing using assay formats that are traditionally considered to be incompatible with flow-based devices (e.g., chemical reactions). The central hypothesis of this research is that the material properties of the paper (e.g., porosity) and the transport of reaction products (e.g., by diffusion) will ultimately control the analytical performance of these paper-based biosensors. By studying these variables systematically, new strategies for low-cost, paper-based biosensing will be developed. Assays at fluidic interfaces will result in the formation of visible colors that will allow users to easily interpret the results of an assay via visual threshold (qualitative), visual comparisons of intensities to read guides (semi-quantitative), or with image analysis (quantitative). This research is significant because the devices derived from this program span a broad range of applications in bioanalysis and will serve as versatile tools for all forms of analytical chemistry. Potential applications include: (i) environmental monitoring of heavy metals, (ii) food security and nutrient density, (iii) detection of nucleic acids and proteins, and (iv) the development of diagnostic assays that are designed specifically for use in limited-resource settings.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在资源有限的环境中，使用现代仪器进行生化分析是不切实际的，在这些环境中，电力和训练有素的操作员可能无法获得，并且分析解决方案必须是低成本的，便携式的，操作简单的。从根本上说，需要专门为应对资源有限环境的挑战而设计的新方法。利用纸作为基底来开发生物传感器是有吸引力的，因为生产它们所需的资源是最少的，并且材料是普遍存在的和廉价的。该项目将创建新的设备设计，可以实现基于纸张的生物传感。将使用疏水（非疏水）屏障在纸上对亲水（疏水）通道进行图案化，以通过芯吸控制样品液体的运输。除了在资源有限的环境中为生物分析设计新的策略外，这项研究计划的更广泛的影响包括为中学生创造教育机会（实习工作坊），高中生（设备设计挑战和暑期实习），以及科学教师由于它们的低成本和易用性，纸基微流体装置作为开发使用点生物测定的平台已经获得关注。独立于检测开发的进展，纸基设备本身，尽管其巨大的潜力，以促进生物分析，已经收到了令人惊讶的关注。该项目旨在建立一个综合的研究和教育计划，重点是开发新型纸基微流体设备，该设备可以促进基于在会聚液体前沿之间形成的界面处发生的化学和生物化学反应的生物传感。利用这种方法的装置将促进使用传统上被认为与基于流的装置不兼容的测定格式的多路生物传感（例如，化学反应）。这项研究的中心假设是，纸张的材料特性（例如，孔隙率）和反应产物的输送（例如，通过扩散）将最终控制这些纸基生物传感器的分析性能。通过系统地研究这些变量，将开发出低成本的纸基生物传感新策略。在流体接口处的测定将导致形成可见颜色，这将允许用户通过视觉阈值（定性）、强度与读取指南的视觉比较（半定量）或图像分析（定量）来容易地解释测定结果。这项研究意义重大，因为从该计划中衍生的设备涵盖了生物分析中的广泛应用，并将作为各种形式的分析化学的通用工具。潜在的应用包括：（i）重金属的环境监测，（ii）食品安全和营养密度，（iii）核酸和蛋白质的检测，以及（iv）专门为有限资源环境设计的诊断测定的开发。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。