Bioinspired Multiplexed Ultrasensitive Biosensing

下一代疾病检测需要变革性的生物传感技术，这些技术是强大的、高度敏感的、基于多个生物标记物的，并且可以在护理点部署。在Biosense中，我建议通过开展一项研究计划来满足这些需求，该计划可同时检测具有极高灵敏度(超灵敏)的多个生物标志物(多路传输)，并将这些多路传输的超灵敏诊断平台开发成有可能集成到在线护理路径中并实现以患者为中心的数字孪生兄弟的护理点设备。变革性的技术平台从生物学中获得灵感，开启了疾病诊断的新纪元。Biosense通过一个三管齐下的工作计划追求这一雄心勃勃的目标：首先，Biosense将我在催化纳米颗粒技术方面的开创性进展与基因编辑方面的前沿发展结合起来，开发出一种同时检测非编码RNA和蛋白质的诊断设备，我将这项技术称为MultiD。其次，Biosense开创了超灵敏的核酸模式检测技术，称为NEXTDx。增强多维和NEXTDx是开发新的催化纳米颗粒，并支持最先进的方法，包括内部开发的新成像技术、从原子级向上的分层表征、原子化和粗粒度计算模型，以及共同实现基础科学发现和翻译成果的尖端数据科学方法。总的来说，Biosense释放了超灵敏生物传感的潜力，具有广泛的临床和社会影响。

Next generation disease detection requires transformative biosensing technologies that are robust, highly sensitive, based on multiple biomarkers, and deployable at the point of care. In bioSense, I propose to meet these needs by pursuing a research programme that simultaneously detects multiple biomarkers ('multiplexed') with extremely high sensitivity ('ultrasensitive'), and develops these multiplexed ultrasensitive diagnostic platforms into point of care devices that have the potential to integrate into online care pathways and enable a patient-centric digital twin. The transformative technological platforms take inspiration from biology to usher in a new era of disease diagnosis. bioSense pursues this ambitious aim through a three-pronged work programme: First, bioSense couples my seminal advances in catalytic nanoparticle technology together with leading-edge developments in gene editing to develop a diagnostic device for the simultaneous detection of noncoding RNA and proteins, a technology I term multiD. Second, bioSense pioneers technology for ultrasensitive detection of nucleic acid patterns, termed NEXTDx. Enhancing multiD and NEXTDx is the development of new catalytic nanoparticles, and underpinning state of the art methodologies including novel imaging technologies developed in-house, hierarchical characterization from atom-level upward, atomistic and coarse-grained computational modelling, and cutting-edge data science approaches that together enable fundamental scientific discoveries and translational outcomes. Collectively, bioSense unlocks the potential of ultrasensitive biosensing for broad clinical and societal impact.