Characterization of the dynamic reorganization of the spindle pole body from a microtubule- to an actin organization center and its contribution to fungal spore morphology

纺锤体极体从微管到肌动蛋白组织中心动态重组的表征及其对真菌孢子形态的贡献

• 批准号: 236448179
• 负责人: Privatdozent Dr. Hans-Peter Schmitz
• 金额: --
• 依托单位: Arbeitsgruppe Genetik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/236448179?language=en
• 关键词: Characterization; dynamic; reorganization; spindle; pole

Fungal spores play an important role for dispersal of fungi and fungal disease. They come in a wide variety of shapes and sizes that are often specialized for a specific route of dispersal. How more complex spore morphologies that differ from a simple round shape are generated is not understood. Using the needle shaped spores of the filamentous growing ascomycete Ashbya gossypii as a model, we could show that for formation of needle shaped spores the remodeling of the outer layers of the spindle pole body (SPB, the fungal analog to the centrosome, which is the microtubule organization center during vegetative growth) to an actin organization center is an essential step. The actin cables formed by this center are necessary to correctly form the tip-region of the needle-shaped spore. A key factor for this remodeling process is the binding of the formin protein AgBnr2 to the SPB, which in turn connects actin cables to the SPB. The experiments outlined in this proposal are designed to understand the precise nature and the timing of the reorganization of the SPB during sporulation.

真菌孢子对于真菌和真菌病害的传播起着重要作用。它们有各种各样的形状和大小，通常专门用于特定的传播途径。与简单圆形不同的更复杂的孢子形态是如何产生的尚不清楚。以丝状生长的子囊菌 Ashbya gossypii 的针状孢子为模型，我们可以表明，为了形成针状孢子，纺锤体极体（SPB，真菌的中心体类似物，是营养生长过程中的微管组织中心）外层重塑为肌动蛋白组织中心是至关重要的一步。由该中心形成的肌动蛋白缆对于正确形成针状孢子的尖端区域是必要的。这种重塑过程的一个关键因素是福尔明蛋白 AgBnr2 与 SPB 的结合，进而将肌动蛋白电缆连接到 SPB。本提案中概述的实验旨在了解孢子形成过程中 SPB 重组的精确性质和时间。