Regulation of the actin-based dynamics of mitochondria by myosin XIX

肌球蛋白 XIX 对线粒体基于肌动蛋白的动力学的调节

• 批准号: 236777323
• 负责人: Professor Dr. Martin Bähler
• 金额: --
• 依托单位: Institut für Molekulare Zellbiologie (aufgelöst)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/236777323?language=en
• 关键词: Regulation; actin; based; dynamics; mitochondria

Mitochondria supply the energy that is needed to keep eukaryotic cells alive. Furthermore, they are involved in intermediary metabolism, calcium signaling, and apoptosis. Mitochondrial dysfunction leads to pleiotropic defects in multicellular organisms with some tissues being more affected than others often giving rise to degenerative diseases. The morphology of mitochondria and distribution within cells is highly dynamic and regulated. Motor molecules move mitochondria along microtubules and actin filaments. In mammalian cells mitochondrial movement along microtubules is relatively well established. The relevant motor molecules have been identified and their mode of association with mitochondria has been partially elucidated. On the other hand, the movement of mitochondria along actin filaments is little studied. Myosin XIX (Myo19), a recently discovered member of the actin-based motor molecule superfamily, has been reported to associate with mitochondria. We plan to study the contribution of Myo19 to the dynamics and movement of mitochondria. Further, we will try to elucidate how Myo19 is interacting with mitochondria. Preliminary data suggest that a mitochondrial protein is involved that is also binding to a microtubule-based motor. This finding raises the question of how actin- and microtubule-dependent movements of mitochondria are coordinated.

线粒体提供维持真核细胞存活所需的能量。此外，它们还参与中间代谢、钙信号传导和细胞凋亡。线粒体功能障碍导致多细胞生物体中的多效性缺陷，其中一些组织比其他组织受到更多影响，通常引起退行性疾病。线粒体的形态和在细胞内的分布是高度动态和受调节的。马达分子使线粒体沿着微管和肌动蛋白丝运动。在哺乳动物细胞中，线粒体沿着微管运动是相对完善的。相关的马达分子已被确定，其与线粒体的关联模式已部分阐明。另一方面，线粒体沿沿着肌动蛋白丝的运动研究很少。肌球蛋白XIX（Myo19）是新近发现的肌动蛋白运动分子超家族成员，与线粒体有密切的联系。我们计划研究Myo19对线粒体动力学和运动的贡献。此外，我们将试图阐明Myo19如何与线粒体相互作用。初步数据表明，一种线粒体蛋白也与微管马达结合有关。这一发现提出了一个问题，即线粒体的肌动蛋白和微管依赖性运动是如何协调的。