Compressed Sub-Aperture Super-Resolution Microscopy

研究人员建议利用光学显微镜领域的最新发展，设计一种新型的反射模式成像仪器，该仪器集成了压缩传感能力，从而能够开发出一种可以用于眼睛和其他生物目标的超分辨率成像的系统。扫描激光眼底镜(SLO)是视网膜诊断成像的主要手段。它基于共聚焦激光扫描显微镜，最近已与自适应光学技术相结合，以提供更清晰的视网膜图像。该项目将1)开发使用光学重新分配反射共聚焦显微镜所需的概念和技术。这将导致扫描激光检眼镜(ORSLO)进行超分辨率视网膜反射成像。2)开发了一种基于数字微镜技术的新型光学结构，增强了ORSLO的性能。这将使视网膜光感受器细胞和血管系统可视化。3)在自适应光学扫描激光检眼镜(AOSLO)中实现了基于压缩传感技术的反射式图像扫描显微镜(ISM)。这将实现超分辨率自适应光学成像。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

The investigators propose to take advantage of very recent developments in the field of optical microscopy and engineer a novel reflectance mode imaging instrument integrated with compressed sensing capability enabling the development of a system that can be used for super-resolved imaging of the eye and other biological targets. Scanning laser ophthalmoscopy (SLO) is a mainstay of diagnostic imaging of the retina. It is based on confocal laser scanning microscopy and has recently been combined with adaptive optics technology to provide sharper images of the retina.The project will 1) Develop the concepts and technology necessary to use optical reassignment reflectance confocal microscopy. This will lead to scanning laser ophthalmoscope (ORSLO) for super-resolved retinal reflectance imaging. 2) Enhance ORSLO by developing a novel optical architecture based on digital micro-mirror technology. This will permit the visualization of retinal photoreceptor cells and vasculature. 3) Implement reflectance image scanning microscopy (ISM) with a compressed sensing technique into an adaptive optics scanning laser ophthalmoscope (AOSLO). This will enable super-resolved adaptive optical imaging.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.