Gigapixel Widefield Super-Resolved Structured Illumination Microscopy

1403905 Izatt，Joseph A.标题：千兆像素宽视场超分辨结构照明显微镜意义：授予杜克大学一个奖项，以进行研究，以改善显微镜技术，在宽视场内以高分辨率对生物标本进行成像。许多广泛使用的显微镜应用，如细胞生物学和组织学，需要在宽视场（FOV）和窄视场之间频繁切换，以识别组织特征和感兴趣的位置，以细胞和亚细胞分辨率成像。手动放大和缩小的需要是耗时的，并且与现代数字图像捕获和查看方法的改进不相容。这项研究地址，并提供了一个解决方案，为这个问题的技术描述：PI将开发一种新的空间结构照明元件放置在靠近样品的多路复用高空间频率的图像内容到检测孔的宽视场显微镜物镜。该项目将推动相干超分辨率成像技术的发展。该项目将包括将现代超分辨率成像技术从目前的纳米级成像扩展到生物研究和临床实验室诊断的更广泛应用。该项目还将支持来自杜克和其他地方的工程专业学生的教育和科学培训。该项目将包括博士。杜克生物医学工程专业学生的论文研究，杜克本科工程专业学生将有机会通过独立学习参与研究。此外，三名被招募到杜克大学本科生研究经验项目的工程专业学生将分别花一个夏天的时间协助这项研究，并参加相关的培训和推广活动。

1403905Izatt, Joseph A.Title: Gigapixel Widefield Super-Resolved Structured Illumination MicroscopySignificance:An award is made to Duke University to perform research to improve microscopy technology for imaging biological specimens over wide fields of view at high resolution. Many widely used applications of microscopy such as in cell biology and histology require frequent switching between a wide field-of-view (FOV) to identify tissue features and locations of interest, and narrow FOV to image at cellular and sub-cellular resolution. The need to manually zoom in and out is time consuming and incompatible with improvements in modern digital image capture and review methodology. This research addresses and provides a solution for this problemTechnical Description:The PI will develop a novel spatially structured illumination element to be placed in close proximity to the sample to multiplex high spatial frequency image content into the detection aperture of a wide field of view microscope objective. The project will advance the state of the art in coherent super-resolution imaging technology. The project will include extending modern super-resolution imaging techniques from their current focus on nanometer scale imaging to broader applications in biological research and clinical laboratory diagnostics. The project will also support the education and scientific training of engineering students from Duke and elsewhere at multiple levels. The project will comprise the Ph.D. dissertation research of a Duke biomedical engineering student, and Duke undergraduate engineering students will have opportunities to participate in the research through independent study. Additionally, three underrepresented engineering students recruited into Duke's Research Experience for Undergraduate program will each spend a summer assisting with this research as well as participating in related training and outreach activities.