Gigapixel Widefield Super-Resolved Structured Illumination Microscopy

1403905Izatt, Joseph a .标题：千兆像素宽视场超分辨结构照明显微镜意义：杜克大学获得了一项奖励，以进行研究，以改进在高分辨率宽视场上成像生物标本的显微镜技术。许多广泛使用的显微镜应用，如细胞生物学和组织学，需要在宽视场（FOV）和窄视场（FOV）之间频繁切换，以识别组织特征和感兴趣的位置，以细胞和亚细胞分辨率成像。手动放大和缩小的需要是耗时的，并且与现代数字图像捕获和审查方法的改进不兼容。技术描述：PI将开发一种新型的空间结构照明元件，将其放置在靠近样品的位置，以将高空间频率图像内容多路复用到宽视场显微镜物镜的检测孔径中。该项目将推动相干超分辨率成像技术的发展。该项目将包括将现代超分辨率成像技术从目前的纳米级成像扩展到生物研究和临床实验室诊断的更广泛应用。该项目还将在多个层面上支持杜克大学和其他地方的工科学生的教育和科学培训。该项目将包括杜克大学生物医学工程专业学生的博士论文研究，杜克大学本科工程专业学生将有机会通过独立学习参与研究。此外，杜克大学本科研究经验项目招募的三名未被充分代表的工程专业学生将每人花一个夏天的时间协助这项研究，并参加相关的培训和推广活动。

1403905Izatt, Joseph A.Title: Gigapixel Widefield Super-Resolved Structured Illumination MicroscopySignificance:An award is made to Duke University to perform research to improve microscopy technology for imaging biological specimens over wide fields of view at high resolution. Many widely used applications of microscopy such as in cell biology and histology require frequent switching between a wide field-of-view (FOV) to identify tissue features and locations of interest, and narrow FOV to image at cellular and sub-cellular resolution. The need to manually zoom in and out is time consuming and incompatible with improvements in modern digital image capture and review methodology. This research addresses and provides a solution for this problemTechnical Description:The PI will develop a novel spatially structured illumination element to be placed in close proximity to the sample to multiplex high spatial frequency image content into the detection aperture of a wide field of view microscope objective. The project will advance the state of the art in coherent super-resolution imaging technology. The project will include extending modern super-resolution imaging techniques from their current focus on nanometer scale imaging to broader applications in biological research and clinical laboratory diagnostics. The project will also support the education and scientific training of engineering students from Duke and elsewhere at multiple levels. The project will comprise the Ph.D. dissertation research of a Duke biomedical engineering student, and Duke undergraduate engineering students will have opportunities to participate in the research through independent study. Additionally, three underrepresented engineering students recruited into Duke's Research Experience for Undergraduate program will each spend a summer assisting with this research as well as participating in related training and outreach activities.