Controlled Synthesis of Polypeptides from Non-Purified N-Carboxyanhydrides
由未纯化的 N-羧酸酐控制合成多肽
基本信息
- 批准号:1905097
- 负责人:
- 金额:$ 23.6万
- 依托单位:
- 依托单位国家:美国
- 项目类别:Standard Grant
- 财政年份:2020
- 资助国家:美国
- 起止时间:2020-06-01 至 2022-05-31
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
In this project, funded by the Macromolecular, Supramolecular and Nanochemistry Program in the Division of Chemistry, Professor Jianjun Cheng of the Department of Chemistry at the University of Illinois at Urbana Champaign is developing fast, inexpensive and simple routes for the preparation of synthetic polypeptides using amino acids as the starting materials. Polypeptides are important biopolymers; They consist of long chains of amino acids linked together by a particular kind of a chemical bond called a peptide bond or link. All living organisms are composed of polypeptides. This research allows for the large-scale preparation of polypeptide materials in several hours instead of days. Even more importantly, this new synthetic protocol eliminates the need for purification of starting materials which is often necessary in order to eliminate numerous side reactions that limit the chain length of the desired polypeptides. Successful advancement of this new method has the potential to impact the synthetic peptide synthesis, as well as several other scientific disciplines. Research activities provide undergraduate and graduate students with valuable educational experience in synthetic polymer chemistry. The knowledge of polymerization chemistry and applications will be disseminated to underrepresented undergraduates and K-12 students through the Discover Engineering Summer Camp programs and Engineering Open-House Program at University of Illinois. This research is focused on developing the open-air method for controlled synthesis of polypeptides from non-purified N-carboxyanhydride. Special emphasis is placed on water-free conditions and the elimination of purification steps involving monomers, initiators and catalysts. These steps are often critical in polymer synthesis in order to minimize chain transfer or termination reactions which severely decrease the degree of polymerization of the polypeptides. Each reaction component is carefully analyzed through systematic studies. These polymerizations are applicable to a wide range of amino acids and enable the synthesis of polypeptides with high molecular weights. The in situ purification can potentially be applied to other polymerization techniques to simplify the procedures and lower the materials synthesis costs. This innovative research has a potential to advance industrially-important rapid syntheses of polypeptides.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
在该项目中,由化学系大分子,超分子和纳米化学计划资助,伊利诺伊大学厄巴纳尚潘分校化学系的程建军教授正在开发快速,廉价和简单的路线,用于以氨基酸为起始原料制备合成多肽。 多肽是重要的生物聚合物;它们由氨基酸长链组成,通过一种特殊的化学键连接在一起,称为肽键或链接。 所有生物体都是由多肽组成的。 这项研究允许在几个小时而不是几天内大规模制备多肽材料。 甚至更重要的是,这种新的合成方案消除了对纯化起始材料的需要,而纯化起始材料通常是消除限制所需多肽的链长的许多副反应所必需的。 这种新方法的成功发展有可能影响合成肽的合成,以及其他几个科学学科。 研究活动为本科生和研究生提供合成高分子化学方面的宝贵教育经验。 聚合化学和应用的知识将通过发现工程夏令营计划和伊利诺伊大学的工程开放计划传播给代表性不足的本科生和K-12学生。本研究旨在建立一种以非纯N-羧酸酐为原料,控制合成多肽的方法。 特别强调无水条件和消除涉及单体、引发剂和催化剂的纯化步骤。 这些步骤在聚合物合成中通常是关键的,以使严重降低多肽聚合度的链转移或终止反应最小化。 通过系统研究仔细分析每个反应组分。 这些聚合适用于广泛的氨基酸,并能够合成具有高分子量的多肽。 原位纯化可以潜在地应用于其他聚合技术,以简化程序和降低材料合成成本。 这一创新性研究有可能推动具有工业重要性的多肽快速合成。该奖项反映了NSF的法定使命,并通过使用基金会的知识价值和更广泛的影响审查标准进行评估,被认为值得支持。
项目成果
期刊论文数量(3)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Paul Braun其他文献
Properties of Optical Data from Activated Partial Thromboplastin Time and Prothrombin Time Assays
活化部分凝血活酶时间和凝血酶原时间测定的光学数据的特性
- DOI:
- 发表时间:
1997 - 期刊:
- 影响因子:6.7
- 作者:
Paul Braun;T. Givens;Andrew Stead;Lisa Beck;Sheila Gooch;R. Swan;T. Fischer - 通讯作者:
T. Fischer
Predicting the presence of plasma heparin using neural networks to analyze coagulation screening assay optical profiles
使用神经网络预测血浆肝素的存在以分析凝血筛查测定光学轮廓
- DOI:
10.1016/s0010-4825(96)00023-6 - 发表时间:
1996 - 期刊:
- 影响因子:7.7
- 作者:
T. Givens;Paul Braun;T. Fischer - 通讯作者:
T. Fischer
Binding properties of hirudin determined by gel filtration and gel electrophoresis.
通过凝胶过滤和凝胶电泳测定水蛭素的结合特性。
- DOI:
- 发表时间:
1990 - 期刊:
- 影响因子:7.5
- 作者:
Paul Braun - 通讯作者:
Paul Braun
The ostracod distribution in the Sea of Galilee (Levant): species distribution and post-mortem dispersal of valves and carapaces
- DOI:
10.1007/s10933-024-00346-8 - 发表时间:
2024-10-26 - 期刊:
- 影响因子:1.300
- 作者:
Steffen Mischke;Paul Braun;Emi Ito;Ahuva Almogi-Labin - 通讯作者:
Ahuva Almogi-Labin
Paul Braun的其他文献
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{{ truncateString('Paul Braun', 18)}}的其他基金
FMRG: Holistic Design of Low-Cost and Recyclable High Energy Density Li-ion Batteries
FMRG:低成本、可回收的高能量密度锂离子电池的整体设计
- 批准号:
2037898 - 财政年份:2021
- 资助金额:
$ 23.6万 - 项目类别:
Standard Grant
MRI: Acquisition of a Multi-Length Scale Ultra High-Resolution X-Ray Nanotomography Instrument
MRI:获取多长度尺度超高分辨率 X 射线纳米断层扫描仪器
- 批准号:
0721324 - 财政年份:2007
- 资助金额:
$ 23.6万 - 项目类别:
Standard Grant
相似国自然基金
新型滤波器综合技术-直接综合技术(Direct synthesis Technique)的研究及应用
- 批准号:61671111
- 批准年份:2016
- 资助金额:58.0 万元
- 项目类别:面上项目
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D-amino acids and beta-amino acids : a new frontier in Ribosomal synthesis of non-standard polypeptides
D-氨基酸和β-氨基酸:非标准多肽核糖体合成的新领域
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- 批准号:
10322150 - 财政年份:2005
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Enabling synthesis and biological studies of homogeneous heparan sulfate and chondroitin sulfate glyco-polypeptides and proteoglycans
实现均质硫酸乙酰肝素和硫酸软骨素糖多肽和蛋白聚糖的合成和生物学研究
- 批准号:
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Enabling synthesis and biological studies of homogeneous heparan sulfate and chondroitin sulfate glyco-polypeptides and proteoglycans
实现均质硫酸乙酰肝素和硫酸软骨素糖多肽和蛋白聚糖的合成和生物学研究
- 批准号:
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- 批准号:
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$ 23.6万 - 项目类别:
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CAREER: Transition Metal Initiators for the Synthesis of Well-Defined Polypeptides
职业:用于合成明确多肽的过渡金属引发剂
- 批准号:
9701969 - 财政年份:1997
- 资助金额:
$ 23.6万 - 项目类别:
Continuing Grant
Design and synthesis of bioactive polypeptides
生物活性多肽的设计与合成
- 批准号:
3793-1991 - 财政年份:1993
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Preparation of Catalytic Antibodies toward the Synthesis of Sequential Polypeptides
序列多肽合成的催化抗体的制备
- 批准号:
04453113 - 财政年份:1992
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$ 23.6万 - 项目类别:
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- 批准号:
3793-1991 - 财政年份:1992
- 资助金额:
$ 23.6万 - 项目类别:
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Synthesis, Characterization and Processing of Rigid Polypeptides of Unusual Geometry
特殊几何形状刚性多肽的合成、表征和加工
- 批准号:
9202434 - 财政年份:1992
- 资助金额:
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