Preparation of Catalytic Antibodies toward the Synthesis of Sequential Polypeptides
序列多肽合成的催化抗体的制备
基本信息
- 批准号:04453113
- 负责人:
- 金额:$ 3.58万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for General Scientific Research (B)
- 财政年份:1992
- 资助国家:日本
- 起止时间:1992 至 1993
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Monoclonal antibodies were raised against peptides containing nonnatural amino acids carring azobenzene group and pyrenyl group, respectively. The anti-azobenzene antibody bound only trans azobenzene group and released it when it was photoisomerized to the cis form. The photoisomerization of bound azobenzene group was found to take place inside the binding site. By using this unique property, a photoswitching of redox enzyme reaction was attempted. When an azobenzene unit was linked to an NAD^+-derivative, the latter showed normal mediation activity in a coupled redox enzyme reaction of alcohol dehydrogenase and diaphorase. When the anti-azobenzene antibody was added to the enzyme system, the mediation activity was completely suppressed in the dark, but the mediation activity recovered when the system was irradiated with UV light. The photocycle could be repeated many times.The photocontrolled antigen-antibody reaction was carried out on a monolayr containing azobenzene groups cast on a flat surface of mica. The antibodies bound on the monolayr surface were directly observed with atomic-force microscopy (AFM) technique. The number of antibody increased with increasing the hapten content, indicating that the binding is specific to the hapten groups. Photocontrol of the hapten-antibody reaction was also achieved on the monolayr surface.The anti-pyrenyl antibody bound a variety of pyrenyl derivatives including L-1-pyrenylalanine. But it did not bind D-1-pyrenylalanine. The unique property will be applied to optical resolution of nonnatural amino acids. The pyrenyl fluorescence of pyrenyl group bound to the anti-pyrenyl antibody was studied in detail. It was found that the pyrenyl group in the excited state remains inside the binding site and fluoresces.
分别制备了抗含偶氮苯基和芘基非天然氨基酸多肽的单克隆抗体。抗偶氮苯抗体仅结合反式偶氮苯基团,并且当其光异构化为顺式形式时将其释放。结合偶氮苯基团的光异构化发生在结合位点内部。利用这种独特的性质,尝试了氧化还原酶反应的光开关。当偶氮苯单元与NAD^+衍生物连接时,后者在醇脱氢酶和心肌黄酶的偶联氧化还原酶反应中显示出正常的介导活性。当将抗偶氮苯抗体添加到酶体系中时,在黑暗中的介导活性被完全抑制,但是当用UV光照射该体系时,介导活性恢复。这种光循环可以重复多次,光控抗原抗体反应是在一个含偶氮苯基的单层膜上进行的。用原子力显微镜(AFM)直接观察抗体结合在单层表面的情况。抗体的数量随着半抗原含量的增加而增加,表明结合对半抗原基团具有特异性。半抗原-抗体反应在单分子膜表面也实现了光控制,抗芘抗体结合了包括L-1-芘丙氨酸在内的多种芘衍生物。但不与D-1-芘丙氨酸结合。这种独特的性质将被应用于非天然氨基酸的光学拆分。详细研究了抗芘基抗体结合芘基的芘基荧光。发现处于激发态的芘基保留在结合位点内并发出荧光。
项目成果
期刊论文数量(50)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
M.Imaizumi, M.Harada, and M.Sisido: "Fluorescence Behavior of Pyrenyl Groups bound to Anti-Pyrenyl Antibody" (in preparation).
M.Imaizumi、M.Harada 和 M.Sisido:“与抗芘基抗体结合的芘基团的荧光行为”(准备中)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
共著(M.Sisido): "New Functionality Materials Vol.B “Synthesis and Function Control of Biofunctionality Materials"" Elsevier Science Publishers B.V., (1993)
合著者 (M.Sisido):“新功能材料 Vol.B “生物功能材料的合成和功能控制””Elsevier Science Publishers B.V.,(1993)
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Hohsaka: "Adaptability of nonnatural aromatic amino acids to the active center of the E.Coli ribosomal A site" FEBS Letter. 335. 47-50 (1993)
T.Hohsaka:“非天然芳香族氨基酸对大肠杆菌核糖体 A 位点活性中心的适应性”FEBS Letter。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
T.Hohsaka: "Adaptability of nonnatural aromatic amino acids to the active center of the E.coli ribosomal A site" FEBS Letters. 335. 47-50 (1993)
T.Hohsaka:“非天然芳香族氨基酸对大肠杆菌核糖体 A 位点活性中心的适应性”FEBS Letters。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
M.Sisido: "Molecular to Supramolecular Design of Synthetic Polypeptides" Progress in Polymer Science. 17. 699-764 (1992)
M.Sisido:“合成多肽的分子到超分子设计”高分子科学进展。
- DOI:
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- 影响因子:0
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SISIDO Masahiko其他文献
SISIDO Masahiko的其他文献
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{{ truncateString('SISIDO Masahiko', 18)}}的其他基金
Construction of Drug-Discovering System by using Multiple Fluorescent Labels on Peptide Library
利用肽库上的多种荧光标记构建药物发现系统
- 批准号:
20241054 - 财政年份:2008
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Chemical Expansion of the Central Dogma towards Synthetic Microorganisms
中心法则对合成微生物的化学扩展
- 批准号:
15101008 - 财政年份:2003
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (S)
Extension of Protein Biosynthesizing System and Incorporation of Nonnatural Amino Acids into Proteins for Chemical Extension of Protein Functions
蛋白质生物合成系统的扩展以及将非天然氨基酸掺入蛋白质中以化学扩展蛋白质功能
- 批准号:
11102003 - 财政年份:1999
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Specially Promoted Research
Preparation of Alloproteins Endowed with Artificial Functions
赋予人工功能的同种异体蛋白的制备
- 批准号:
06403034 - 财政年份:1994
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Asymmetric Photoprocesses in Chiral Biopolymer Matrices and Possible Application to Chiroptical Photorecording
手性生物聚合物基质中的不对称光过程及其在手性光学光记录中的可能应用
- 批准号:
63470095 - 财政年份:1988
- 资助金额:
$ 3.58万 - 项目类别:
Grant-in-Aid for General Scientific Research (B)