NSF Postdoctoral Fellowship in Biology FY 2019: Deciphering CLE Peptide Signaling Pathways in Sunflower (Helianthus annuus)

2019 财年 NSF 生物学博士后奖学金:破译向日葵(Helianthus annuus)中的 CLE 肽信号通路

基本信息

  • 批准号:
    1906389
  • 负责人:
  • 金额:
    $ 21.6万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
    Fellowship Award
  • 财政年份:
    2019
  • 资助国家:
    美国
  • 起止时间:
    2019-07-01 至 2022-06-30
  • 项目状态:
    已结题

项目摘要

This action funds an NSF National Plant Genome Initiative Postdoctoral Research Fellowship in Biology for FY 2019. The fellowship supports a research and training plan in a host laboratory for the Fellow who also presents a plan to broaden participation in biology. The title of the research and training plan for this fellowship to Dr. Daniel Scott Jones is "Deciphering CLE peptide signaling pathways in sunflower (Helianthus annuus)". The host institution for the fellowship is the University of North Carolina in Chapel Hill and the sponsoring scientist is Dr. Zachary Nimchuk. Unlike animals, plants continue to develop and generate new organs throughout their lifespan. To support this continual growth, plants maintain a functional set of stem cells that can divide and become any other type of cell within the plant as needed; giving rise to all cells found in stems, leaves, roots, flowers, and seeds. Even slight changes in the number of stem cells maintained in the shoot of a plant can have a direct impact on the number and size of organs it produces. Understanding how stem cell identity is controlled in plants is directly linked with our ability to influence agronomically important traits such as fruit size, grain yield or even overall crop health and performance. This project aims to uncover key genes regulating stem cell identity in sunflower, an important seed and oil crop. During the course of this project, tools will be developed to further the use of sunflower as a model research system for studying species within the sunflower family (Asteraceae), one of the largest plant families with great economic significance/potential. Support provided during this fellowship will also enable research opportunities specifically catered toward first-generation college students at the host institution. Training objectives include acquiring new skills in comparative genomics, development, epigenetics and bioinformatics. Understanding how conserved signaling pathways mediate cell-cell communication to coordinate diverse developmental forms is a central question within the biology. CLE (CLAVATA3/Endosperm surrounding region-related) peptide signaling represents a conserved pathway regulating stem cell identity and organogenesis across highly divergent species. The main objectives of this project are to: 1) identify CLE signaling components expressed during inflorescence development in sunflower; 2) define signaling outputs in sunflower shoot and root meristems in response to CLE peptides via RNA-seq and ATAC -seq; 3) implement CRISPR-Cas9 mutagenesis in sunflower and functionally analyze key CLE pathway components; 4) complete an intensive scientific training program, developing new skills in comparative and large-scale genomics while producing foundational data from which to build an independent research program. All genomic datasets generated during the course of this study will be deposited into the publicly accessible Gene Expression Omnibus-GEO (http://www.ncbi.nlm.nih.gov/geo/) and will be freely shared with the sunflower community via the Institut National de la Recherche Aronomique (INRA) Sunflower Bioinformatics Resource site (https://www.heliagene.org/). Additionally, all CRISPR-Cas9 constructs, optimized for use in sunflower, will be made available by request and through Addgene, the nonprofit global plasmid repository (https://www.addgene.org/), for ease of distribution. Keywords: sunflower, RNA-seq, ATAC-seq, transcriptome, stem cell, intercellular signaling, inflorescence, flowerThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.
这项行动为2019财年的NSF国家植物基因组计划生物学博士后研究奖学金提供资金。该研究金支持研究员在东道实验室的研究和培训计划,研究员还提出了扩大生物学参与的计划。丹尼尔斯科特琼斯博士的研究和培训计划的标题是“破译向日葵(向日葵)中的CLE肽信号通路"。研究金的主办机构是查佩尔山的北卡罗来纳州大学,赞助科学家是扎卡里·尼姆丘克博士。与动物不同,植物在其整个生命周期中不断发育并产生新的器官。为了支持这种持续的生长,植物维持一组功能性干细胞,这些干细胞可以根据需要分裂并成为植物内任何其他类型的细胞;产生茎,叶,根,花和种子中发现的所有细胞。即使是植物茎中维持的干细胞数量的微小变化也会对其产生的器官的数量和大小产生直接影响。了解植物中干细胞身份是如何控制的,与我们影响重要农艺性状的能力直接相关,例如果实大小,谷物产量甚至整体作物健康和性能。该项目旨在揭示调节向日葵(一种重要的种子和油料作物)干细胞身份的关键基因。在该项目的过程中,将开发工具,以进一步使用向日葵作为研究向日葵科(菊科)物种的模型研究系统,向日葵科是具有巨大经济意义/潜力的最大植物科之一。在此奖学金期间提供的支持也将使研究机会,专门迎合了第一代大学生在主办机构。 培训目标包括获得比较基因组学、发展、表观遗传学和生物信息学方面的新技能。 了解保守的信号通路如何介导细胞间的通讯以协调不同的发育形式是生物学中的一个中心问题。CLE(CLAVATA 3/Endosperm around region-related)肽信号转导代表了在高度分化的物种中调节干细胞身份和器官发生的保守途径。该项目的主要目标是:1)鉴定向日葵花序发育期间表达的CLE信号传导组分; 2)通过RNA-seq和ATAC-seq定义向日葵茎和根分生组织响应CLE肽的信号传导输出; 3)在向日葵中实施CRISPR-Cas9诱变并功能分析关键CLE途径组分; 4)完成强化科学培训计划,开发比较和大规模基因组学的新技能,同时产生基础数据,以建立独立的研究计划。本研究过程中生成的所有基因组数据集将保存在可公开访问的Gene Expression Omnibus-GEO(http://www.ncbi.nlm.nih.gov/geo/)中,并将通过国家Aronomique研究所(INRA)向日葵生物信息学资源网站(https://www.heliagene.org/)与向日葵社区免费共享。此外,所有优化用于向日葵的CRISPR-Cas9构建体将通过请求和非营利全球质粒库Addgene(https://www.addgene.org/)提供,以便于分发。保留字:向日葵,RNA序列,ATAC序列,转录组,干细胞,细胞间信号,花序,花该奖项反映了NSF的法定使命,并已被认为是值得通过使用基金会的智力价值和更广泛的影响审查标准进行评估的支持。

项目成果

期刊论文数量(1)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Daniel Jones其他文献

Lysophospholipid (S1P) receptors (version 2020.5) in the IUPHAR/BPS Guide to Pharmacology Database
IUPHAR/BPS 药理学指南数据库中的溶血磷脂 (S1P) 受体(版本 2020.5)
  • DOI:
    10.2218/gtopdb/f135/2020.5
  • 发表时间:
    2020
  • 期刊:
  • 影响因子:
    2.3
  • 作者:
    V. Blaho;J. Chun;Daniel Jones;Deepa Jonnalagadda;Y. Kihara;Valerie Tan
  • 通讯作者:
    Valerie Tan
Evolving Management of Zenker’s Diverticulum in the Endoscopic Era: A North American Experience
内窥镜时代 Zenker 憩室的不断发展的管理:北美经验
  • DOI:
    10.1007/s00268-016-3442-0
  • 发表时间:
    2016
  • 期刊:
  • 影响因子:
    2.6
  • 作者:
    Daniel Jones;A. Aloraini;S. Gowing;J. Cools;M. Leimanis;R. Tabah;L. Ferri
  • 通讯作者:
    L. Ferri
Making a Fascist Family: Spearhead and the Attempt to Build a Nationalist Community Through Magazine Print Culture
打造法西斯家庭:通过杂志印刷文化建立民族主义社区的先锋和尝试
Special and structured matrices in max-plus algebra
最大加代数中的特殊和结构化矩阵
  • DOI:
  • 发表时间:
    2017
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Daniel Jones
  • 通讯作者:
    Daniel Jones
Management during the First Three Months after Renal Transplantation
肾移植后前三个月的管理
  • DOI:
    10.1002/9781118305294.ch22
  • 发表时间:
    2012
  • 期刊:
  • 影响因子:
    2.1
  • 作者:
    I. Macphee;J. Popoola;Daniel Jones
  • 通讯作者:
    Daniel Jones

Daniel Jones的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Daniel Jones', 18)}}的其他基金

Conference: Rocky Mountain Geobiology Symposium 2024
会议:2024 年落基山地球生物学研讨会
  • 批准号:
    2417156
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Standard Grant
The Gulf Stream control of the North Atlantic carbon sink
湾流对北大西洋碳汇的控制
  • 批准号:
    NE/W009579/1
  • 财政年份:
    2023
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Research Grant
CAREER: Do microbes form caves? Sulfide oxidation and limestone corrosion in sulfuric acid caves
职业:微生物会形成洞穴吗?
  • 批准号:
    2239710
  • 财政年份:
    2023
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Continuing Grant
EAGER: DCL: SaTC: Enabling Interdisciplinary Collaboration: Inoculation vs. education: the role of real time alerts and end-user overconfidence
EAGER:DCL:SaTC:实现跨学科协作:接种与教育:实时警报和最终用户过度自信的作用
  • 批准号:
    2210198
  • 财政年份:
    2022
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Standard Grant
Collaborative Research: RESEARCH-PGR: Comparative genomics of the capitulum: deciphering the molecular basis of a key floral innovation
合作研究:RESEARCH-PGR:头状花序的比较基因组学:破译关键花卉创新的分子基础
  • 批准号:
    2214474
  • 财政年份:
    2022
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Standard Grant
Seabed Mining And Resilience To EXperimental impact
海底采矿和实验影响的恢复能力
  • 批准号:
    NE/T003537/1
  • 财政年份:
    2021
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Research Grant
Autonomous Techniques for anthropogenic Structure Ecological Assessment (AT-SEA)
人为结构生态评估自主技术(AT-SEA)
  • 批准号:
    NE/T010649/1
  • 财政年份:
    2021
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Research Grant
Advaenced state estimats of the ocean and cryosphere: innovative new tools to better understand, predict, and prepare for sea level changes
海洋和冰冻圈的先进状态估计:更好地理解、预测和准备海平面变化的创新工具
  • 批准号:
    MR/T020822/1
  • 财政年份:
    2020
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship
EVIST/HST Individual Awards
EVIST/HST 个人奖
  • 批准号:
    8516282
  • 财政年份:
    1985
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Interagency Agreement
American Chemists and the Geneva Protocol
美国化学家和日内瓦议定书
  • 批准号:
    7614312
  • 财政年份:
    1976
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Standard Grant

相似海外基金

NSF Postdoctoral Fellowship in Biology: Investigating a Novel Circadian Time-Keeping Mechanism Revealed by Environmental Manipulation
美国国家科学基金会生物学博士后奖学金:研究环境操纵揭示的新型昼夜节律机制
  • 批准号:
    2305609
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Chironomid Bioturbation at Future High Temperature Scenarios and its Effect on Nutrient Fluxes and Bacterial Activity
NSF 生物学博士后奖学金:未来高温场景下的摇蚊生物扰动及其对营养通量和细菌活性的影响
  • 批准号:
    2305738
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Understanding the role of dietary toxins in shaping microbial community dynamics in the gut
NSF 生物学博士后奖学金:了解膳食毒素在塑造肠道微生物群落动态中的作用
  • 批准号:
    2305735
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Was there a Tropical Forest in North America after the end-Cretaceous Extinction?
美国国家科学基金会生物学博士后奖学金:白垩纪末期灭绝后北美是否存在热带森林?
  • 批准号:
    2305812
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Investigating the role of thermal stress response in facilitating adaptation in camel spiders
美国国家科学基金会生物学博士后奖学金:研究热应激反应在促进骆驼蜘蛛适应中的作用
  • 批准号:
    2305969
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Human Domestication of Maize as Bio-cultural Coevolution
美国国家科学基金会生物学博士后奖学金:人类驯化玉米作为生物文化协同进化
  • 批准号:
    2305694
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Potential for Diversified Crop Rotations to Promote Solid Phosphorus Cycling in Agroecosystems
美国国家科学基金会生物学博士后奖学金:多样化作物轮作促进农业生态系统固体磷循环的潜力
  • 批准号:
    2305456
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Priority Effects Within and Between Guilds of Fungal Symbionts
NSF 生物学博士后奖学金:真菌共生体内部和之间的优先效应
  • 批准号:
    2305876
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology: Assessment of interactions between nectarivorous birds and flowering plants to investigate pollination loss in Hawaiian forests
美国国家科学基金会生物学博士后奖学金:评估食蜜鸟类和开花植物之间的相互作用,以调查夏威夷森林的授粉损失
  • 批准号:
    2305728
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
NSF Postdoctoral Fellowship in Biology
NSF 生物学博士后奖学金
  • 批准号:
    2305773
  • 财政年份:
    2024
  • 资助金额:
    $ 21.6万
  • 项目类别:
    Fellowship Award
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了