SBIR Phase I: High Throughput Cryopreservation of Aquaculture Seed

SBIR 第一阶段：水产养殖种子的高通量冷冻保存

• 批准号: 1913772
• 负责人: Kalliopi Loutradi - Rolenc
• 金额: $ 22.5万
• 依托单位: Ecto, Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2021-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1913772&HistoricalAwards=false
• 关键词: SBIR; Phase; Throughput; Cryopreservation; Aquaculture

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project will be to advance cryopreservation technologies for the development of a seed banking product for commercially important aquaculture species. Genetic banking of plant cells, tissues, seeds, and embryos is common practice in agriculture to ensure important genetic lines are not lost due to disease outbreak or environmental catastrophe. However, genetic banking of embryos and larvae within the aquaculture industry is nonexistent to date, and the investments made toward genetic improvements are susceptible to catastrophic loss without a proper seed banking product. The inability to reliably preserve seed for long periods of time is currently a barrier to the formation of an aquaculture seed storage product. This research will enhance scientific and technological understanding for the cryopreservation of aquatic organisms and be a transformative step to protect genetic resources vital to the commercial aquaculture industry. These technological advancements also can be adapted for the conservation of threatened or endangered aquatic organisms important to food security and ecosystem health domestically and abroad.This SBIR Phase I project proposes to develop a technology to implement a genetic banking product that allows aquaculture facilities to "cryobank" thousands of seeds from family lines developed over years of selective breeding programs. Recent advancements in rapid cooling for storage at liquid nitrogen temperatures (-196 degrees C) and ultra-rapid rewarming (~107 degrees C/min) have led to major breakthroughs in cryopreservation research. These advancements will be utilized to develop a low throughput, high efficacy strategy for the successful cryopreservation of an important aquaculture species, Litopenaeus vannamei or Pacific white shrimp. Protocol optimization for cryoprotective agent loading/unloading, vitrification, and ultra-rapid warming will result in high revival, survival, and grow out to post larval stages. Once proven, the technique will be scaled up to develop a high throughput process to successfully cryopreserve 10,000 samples per day. This technology will enable the commercial aquaculture industry to conserve important genetic strains and stockpile cryopreserved seed at the levels necessary to avoid interruptions to food supply commonly caused by disease outbreaks and environmental catastrophe.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该小企业创新研究 (SBIR) 项目的更广泛影响/商业潜力将是推进低温保存技术，以开发商业上重要的水产养殖物种的种子库产品。 植物细胞、组织、种子和胚胎的遗传库是农业中的常见做法，以确保重要的遗传系不会因疾病爆发或环境灾难而丢失。然而，迄今为止，水产养殖业中的胚胎和幼体基因库还不存在，如果没有适当的种子库产品，用于基因改良的投资很容易遭受灾难性损失。目前，无法可靠地长期保存种子是形成水产养殖种子储存产品的障碍。这项研究将增强对水生生物低温保存的科学和技术理解，并成为保护对商业水产养殖业至关重要的遗传资源的变革性步骤。 这些技术进步还可用于保护对国内外粮食安全和生态系统健康至关重要的受威胁或濒危水生生物。SBIR 第一阶段项目提议开发一种技术来实施遗传库产品，使水产养殖设施能够“冷冻库”来自经过多年选择性育种计划开发的家族品系的数千个种子。 液氮温度（-196 摄氏度）快速冷却储存和超快速复温（~107 摄氏度/分钟）方面的最新进展导致低温保存研究取得重大突破。这些进步将用于开发低通量、高效的策略，以成功冷冻保存重要的水产养殖物种凡纳滨对虾或太平洋白虾。 冷冻保护剂加载/卸载、玻璃化和超快速升温的方案优化将导致高复活、存活和生长到后幼虫阶段。 一旦经过验证，该技术将扩大规模，开发出高通量工艺，每天成功冷冻保存 10,000 个样本。 这项技术将使商业水产养殖业能够保存重要的遗传品系，并以必要的水平储存冷冻种子，以避免通常因疾病爆发和环境灾难而造成的食品供应中断。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。