MRI: Acquisition of a high performance matrix-assisted laser ionization dual time-of-flight (MALDI/TOF-TOF) mass spectrometer (MS)

该奖项由主要研究仪器（MRI）和化学研究仪器项目支持。来自美国阿拉巴马大学塔斯卡卢萨分校的Carolyn Cassady教授及其同事yuuping Bao， Jason Bara， Marco Bonizzoni和Paul Rupar正在获得一个具有碰撞诱导解离（CID）能力的矩阵辅助激光解吸/电离飞行时间质谱仪（MALDI/TOF-TOF MS）。通常，质谱（MS）是用于鉴定和表征复杂样品中少量化学物质的关键分析方法之一。MALDI TOF结合了温和的电离（非常适合产生肽，蛋白质，核酸，碳水化合物，合成聚合物和其他类似大小的物种的完整离子）与检测模式，在广泛的质量范围内提供了灵敏度和准确性之间的良好平衡。这种高度敏感的技术可以识别和确定复杂混合物中的分子结构。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生使用这种现代分析技术来扩大参与。它还用于外展活动，例如一年一度的阿拉巴马州高级仪器技术研讨会（AITC），该研讨会使整个地区的本科生接触到最先进的仪器。在美国国家科学基金会本科生研究经历（REU）项目中，化学和材料科学专业的学生也使用MALDI/TOF-TOF ms .这种质谱仪可以提高各级别的研究和教育水平。它有助于研究人员开发利用去质子化离子对酸性肽进行分析和测序的技术，以及表征聚合物-碘烯杂合体的结构、分子量和其他特性的技术。MALDI/TOF-TOF质谱用于检测各种生物分子和聚合物的氧化铁纳米颗粒平台。该仪器还用于表征超支化聚电解质的主客体配合物和分析阴离子开环聚合产生的聚亚胺。

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation Programs. Professor Carolyn Cassady from University of Alabama Tuscaloosa and colleagues Yuping Bao, Jason Bara, Marco Bonizzoni and Paul Rupar are acquiring a matrix assisted laser desorption/ionization time-of-flight mass spectrometer with collision induced dissociation (CID) capabilities (MALDI/TOF-TOF MS.) In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide mass range. This highly sensitive technique allows identification and determination of the structure of molecules in a complex mixture. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used in outreach activities, such as the annual Alabama Advanced Instrumental Techniques Colloquium (AITC), which exposes undergraduate students from throughout the region to state-of-the-art instrumentation. Students in an NSF Research experiences for Undergraduates (REU) program in chemistry and materials science also use the MALDI/TOF-TOF MS.This mass spectrometer enhances research and education at all levels. It aids researchers in developing techniques for the analysis and sequencing of acidic peptides using deprotonated ions and for characterizing structures, molecular weights and other properties of polymer-ionene hybrids. The MALDI/TOF-TOF MS is used to examine iron oxide nanoparticle platforms for a variety of biomolecules and polymers. The instrumentation is also used for characterizing host-guest complexes of hyperbranched polyelectrolytes and analyzing polyimines produced by anionic ring opening polymerizations.