MRI: Acquisition of a high performance matrix-assisted laser ionization dual time-of-flight (MALDI/TOF-TOF) mass spectrometer (MS)

该奖项由主要研究仪器(MRI)和化学研究仪器计划支持。阿拉巴马大学塔斯卡卢萨分校的卡罗琳·卡萨迪教授及其同事包玉平、贾森·巴拉、马尔科·博尼佐尼和保罗·鲁帕尔正在获得一台具有碰撞诱导解离(CID)能力的基质辅助激光解吸/电离飞行时间质谱仪(MALDI/TOF-TOF MS)。通常，质谱仪是用于鉴定和表征复杂样品中少量化学物种的关键分析方法之一。MALDI TOF将温和的电离(非常适合生产完整的多肽、蛋白质、核酸、碳水化合物、合成聚合物和其他类似大小物种的离子)与检测模式相结合，在广泛的质量范围内提供了灵敏度和准确性之间的极佳平衡。这种高度灵敏的技术可以识别和确定复杂混合物中的分子结构。此次收购加强了大学和地区的研究基础设施。该仪器通过让不同的学生参与到这一现代分析技术中来扩大参与。它还被用于外展活动，例如一年一度的阿拉巴马州高级仪器技术研讨会(AITC)，它让整个地区的本科生接触到最先进的仪器设备。NSF本科生研究体验(REU)项目化学和材料科学的学生也使用MALDI/TOF-TOF MS。这种质谱计加强了所有级别的研究和教育。它帮助研究人员开发使用去质子化离子对酸性多肽进行分析和排序的技术，以及表征聚合物-紫罗兰烯杂化的结构、相对分子质量和其他性质的技术。MALDI/TOF-TOF MS用于检测各种生物分子和聚合物的氧化铁纳米颗粒平台。该仪器还用于表征超支化聚电解质的主客体络合物和分析由阴离子开环聚合产生的聚亚胺。

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation Programs. Professor Carolyn Cassady from University of Alabama Tuscaloosa and colleagues Yuping Bao, Jason Bara, Marco Bonizzoni and Paul Rupar are acquiring a matrix assisted laser desorption/ionization time-of-flight mass spectrometer with collision induced dissociation (CID) capabilities (MALDI/TOF-TOF MS.) In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide mass range. This highly sensitive technique allows identification and determination of the structure of molecules in a complex mixture. The acquisition strengthens the research infrastructure at the university and regional area. The instrument broadens participation by involving diverse students with this modern analytical technique. It is also used in outreach activities, such as the annual Alabama Advanced Instrumental Techniques Colloquium (AITC), which exposes undergraduate students from throughout the region to state-of-the-art instrumentation. Students in an NSF Research experiences for Undergraduates (REU) program in chemistry and materials science also use the MALDI/TOF-TOF MS.This mass spectrometer enhances research and education at all levels. It aids researchers in developing techniques for the analysis and sequencing of acidic peptides using deprotonated ions and for characterizing structures, molecular weights and other properties of polymer-ionene hybrids. The MALDI/TOF-TOF MS is used to examine iron oxide nanoparticle platforms for a variety of biomolecules and polymers. The instrumentation is also used for characterizing host-guest complexes of hyperbranched polyelectrolytes and analyzing polyimines produced by anionic ring opening polymerizations.