Escaping Bio-Assay Guided Isolation: Nature's Tools for Chemical Biology

摆脱生物测定引导的分离：化学生物学的自然工具

• 批准号: DP160101429
• 负责人: Em/Prof Ronald Quinn
• 金额: $ 34.63万
• 依托单位: Griffith University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-01-01 至 2021-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160101429
• 关键词: Escaping; Bio; Assay; Guided; Isolation

The project aims to transform the approach to identify novel biologically active compounds that occur in nature. For decades, natural product chemistry has centred on bio-assay guided isolation, but it has become increasingly difficult to isolate novel compounds. While de-replication strategies detect the presence of known compounds using databases, more impact would be achieved by directly detecting novel compounds. Nuclear magnetic resonance (NMR) spectroscopy detects every molecule that has a proton and is quantitative. This project plans to develop a NMR technique to escape bio-assay guided isolation by analysing a fraction library. Biotechnology innovation is dependent on novel compounds to provide new products. Replacing ‘grind and find’ with a technique that never lies would be transformational.

该项目旨在改变识别自然界中存在的新型生物活性化合物的方法。几十年来，天然产物化学一直集中在生物测定指导下的分离，但是分离新化合物变得越来越困难。虽然去复制策略使用数据库检测已知化合物的存在，但直接检测新化合物会产生更大的影响。核磁共振（NMR）光谱检测每一个有质子的分子，并且是定量的。该项目计划开发一种核磁共振技术，通过分析一个分数库来逃避生物测定指导的分离。生物技术的创新依赖于新的化合物来提供新的产品。用一种从不说谎的技术取代“刷任务并找到”将是一种转变。