Establishment of ink jet technology to develop high throughput bio-assay system

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A novel bioassay system using ink jet printing technology and a paper substrate has been developed. Ink jet printing has potential for high-precision metered dispensing of bacteria and nutrients in various conditions. Paper has the potential to be used as a container to accommodate bacteria and nutrients and is easily disposed of by combustion after use. Filter paper was first immersed in a toluene solution of polystyrene and hydrophobized after drying. Toluene was printed on a few sections of the filter paper to make them hydrophilic again. An agar medium with its viscosity adjusted by acid hydrolysis was printed on the hydrophilic sections. Bacteria were manually dispensed onto the medium, and the colonies of the bacteria were observed to grow for at least 24 h before the medium dried. Poly(vinyl alcohol) with polymerization initiator was also successfully applied as a medium. This bioassay system is expected to be applied for a variety of bacterial and yeast spores, and enzymes.