Establishment of ink jet technology to develop high throughput bio-assay system

建立喷墨技术开发高通量生物测定系统

• 批准号: 23658138
• 负责人: ENOMAE Toshiharu
• 金额: $ 2.5万
• 依托单位: University of Tsukuba (2012-2013)The University of Tokyo (2011)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2013
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23658138/
• 关键词: バイオアッセイ; インクジェット; バクテリア; 細胞; 寒天培地; 紙; 培地; 大腸菌; ラテックス; アガロース; バイオエタノール

A novel bioassay system using ink jet printing technology and a paper substrate has been developed. Ink jet printing has potential for high-precision metered dispensing of bacteria and nutrients in various conditions. Paper has the potential to be used as a container to accommodate bacteria and nutrients and is easily disposed of by combustion after use. Filter paper was first immersed in a toluene solution of polystyrene and hydrophobized after drying. Toluene was printed on a few sections of the filter paper to make them hydrophilic again. An agar medium with its viscosity adjusted by acid hydrolysis was printed on the hydrophilic sections. Bacteria were manually dispensed onto the medium, and the colonies of the bacteria were observed to grow for at least 24 h before the medium dried. Poly(vinyl alcohol) with polymerization initiator was also successfully applied as a medium. This bioassay system is expected to be applied for a variety of bacterial and yeast spores, and enzymes.

利用喷墨打印技术和纸基，开发了一种新的生物测定系统。喷墨打印具有在各种条件下对细菌和营养物进行高精度计量分配的潜力。纸有可能被用作容纳细菌和营养物质的容器，并且在使用后很容易通过燃烧处理。首先将滤纸浸入聚苯乙烯的甲苯溶液中，干燥后疏水化。将甲苯印刷在滤纸的几个部分上以使它们再次亲水。将其粘度通过酸水解调节的琼脂培养基印刷在亲水部分上。将细菌手动分配到培养基上，观察细菌菌落在培养基干燥前生长至少24小时。还成功地将具有聚合引发剂的聚乙烯醇用作介质。该生物测定系统有望应用于多种细菌和酵母孢子以及酶的测定。

项目成果

Research of paper devices and eco-friendly materials in University of Tsukuba

筑波大学纸装置及环保材料研究

• 发表时间: 2013
• 作者: Tithimanan Srimongkon;Toshiharu Enomae;Toshiharu Enomae
• 通讯作者: Toshiharu Enomae

紙のコート層の微細構造と印刷適性

纸张涂层的微观结构和印刷适性

• 作者: 江前敏晴;ティティマナン スリモンコン;タンチラ ブンヤピパット;エフィ オクタビア;シュ インチャオ;望月有希子;江前敏晴
• 通讯作者: 江前敏晴

生物材料グリーンプロセシング研究について

关于生物材料绿色加工研究

• 发表时间: 2013
• 作者: 江前敏晴;ティティマナン スリモンコン;タンチラ ブンヤピパット;エフィ オクタビア;シュ インチャオ;望月有希子
• 通讯作者: 望月有希子

Bacterial culture system using paper substrate and ink jet printing

使用纸基材和喷墨印刷的细菌培养系统

• 发表时间: 2013
• 作者: Tithimanan Srimongkon;Toshiharu Enomae
• 通讯作者: Toshiharu Enomae

Development of bacterial culture system using paper device for containing media and inkjet printer for dispensing bacteria

开发细菌培养系统，使用纸质装置容纳介质和喷墨打印机分配细菌

• 发表时间: 2013
• 作者: Tithimanan Srimongkon;Toshiharu Enomae
• 通讯作者: Toshiharu Enomae