EDGE TT: Genetic Transformation of Broadleaf Plantain (Plantago major) and Narrowleaf Plantain (Plantago lanceolata)

EDGE TT：阔叶车前草（Plantago Major）和窄叶车前草（Plantago lanceolata）的遗传转化

• 批准号: 1923557
• 负责人: Cankui Zhang
• 金额: $ 45.29万
• 依托单位: Purdue University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1923557&HistoricalAwards=false
• 关键词: EDGE; TT; Genetic; Transformation; Broadleaf

More than 250 species in the Plantago genus are distributed throughout the world, many of which are of broad interest because of their medicinal properties and their ability to reduce toxic heavy metals from soil and water. Plantago species are also widely used as experimental model systems for both basic and applied agriculturally-relevant research. For example, Plantago was one of the first species used to study male sterility, an important reproductive trait that has been exploited for crop improvement. Despite its economic and scientific importance, efficient modern molecular tools for Plantago are lacking and represent a significant bottleneck that hampers efforts to provide an in-depth understanding of the processes and underlying regulatory mechanisms and the fundamental discoveries necessary for future crop improvement. This project aims to provide the tools for genetically testing gene function through the development of efficient plant transformation methods in Plantago. Broader impacts of the project include the wide dissemination of protocols and other project outcomes to the broader plant research communities and the engagement and training of high school students, particularly those from underrepresented groups in science, through participation in existing programs at Purdue. The Plantago genus has many unique features that have led to a series of exciting discoveries, from the initial observation and description of male sterility 143 years ago to studies focused on elucidating unique aspects of sugar transport in plants, and more recently the involvement of vasculature in response to mineral deficiency. In the last two decades, a large number of research groups over the world have conducted transcriptomic, proteomic, metabolomic, and biochemical studies and identified thousands of candidate genes and proteins that likely participate in the diverse biological processes observed in the Plantago genus. This project aims to advance the direct testing of gene function in Plantago by establishing efficient transformation methods in two widely used Plantago species, broadleaf (P. major) and narrowleaf (P. lanceolata) plantains. These two species were chosen because of their popularity as model systems by the Plantago research community, their low ploidy (2x) and small genome sizes, as well as their amenability to genetic modification. All protocols and resources will be made freely available and accessible to the public through a project website, a publicly available video, multiple publications and on-site training workshops. This project is co-funded by the Enabling Discoveries through Genomic Tools (EDGE) and the Plant Genome Research Program (PGRP) in the Directorate for Biological Sciences.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

车前属植物有250多个物种分布在世界各地，其中许多物种因其药用特性和减少土壤和水中有毒重金属的能力而受到广泛关注。车前属物种也被广泛用作基础和应用农业相关研究的实验模型系统。例如，车前是用于研究雄性不育的第一个物种之一，雄性不育是一种重要的生殖性状，已被用于作物改良。尽管其经济和科学的重要性，有效的现代分子工具车前缺乏，并代表了一个重大的瓶颈，阻碍了努力提供深入了解的过程和潜在的监管机制和未来作物改良所需的基本发现。 本项目旨在通过建立有效的车前属植物转化方法，为基因功能的遗传检测提供工具。该项目的更广泛的影响包括协议和其他项目成果的广泛传播，以更广泛的植物研究社区和高中学生的参与和培训，特别是那些来自科学代表性不足的群体，通过参与普渡大学现有的计划。 车前属具有许多独特的特征，这些特征导致了一系列令人兴奋的发现，从143年前对雄性不育的最初观察和描述，到专注于阐明植物中糖运输的独特方面的研究，以及最近参与脉管系统对矿物质缺乏的反应。在过去的二十年里，世界上大量的研究小组进行了转录组学，蛋白质组学，代谢组学和生物化学研究，并确定了数千个候选基因和蛋白质，可能参与在车前草属中观察到的各种生物学过程。该项目旨在通过建立两种广泛使用的车前属物种，阔叶（P. major）和窄叶（P. lanceolata）大蕉的有效转化方法来促进车前属基因功能的直接测试。选择这两个物种是因为它们作为车前草研究社区的模型系统的受欢迎程度，它们的低倍性（2x）和小基因组大小，以及它们对遗传修饰的适应性。所有协议和资源将通过项目网站、公开视频、多种出版物和现场培训讲习班免费提供给公众。该项目由生物科学理事会的基因组工具发现（EDGE）和植物基因组研究计划（PGRP）共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Agrobacterium tumefaciens-Mediated Genetic Transformation of Narrowleaf Plantain

根癌农杆菌介导的窄叶车前草遗传转化

• DOI: 10.3791/64777
• 发表时间: 2023
• 期刊: Journal of Visualized Experiments
• 作者: Levengood, Hannah;Dou, Yanxia;Fan, Jinping;Bajszar, Anna;Huang, Jing;Abbas, Syed Mohsin;Zhou, Yun;Zhang, Cankui
• 通讯作者: Zhang, Cankui