Linking evolutionary processes and taxonomy in the peatmoss group Sphagnum subg. Cuspidata

将泥炭类泥炭藓亚群的进化过程和分类学联系起来。

• 批准号: 1928514
• 负责人: Arthur Shaw
• 金额: $ 82万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-01 至 2024-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1928514&HistoricalAwards=false
• 关键词: Linking; evolutionary; processes; taxonomy; peatmoss

Peatmosses (genus Sphagnum) cover huge areas of the Northern Hemisphere where they help regulate regional patterns of water drainage, and in the far North, the extent of permafrost (permanently frozen ground). Within these Sphagnum-dominated peatland ecosystems, peatmosses store vast amounts of carbon in the form of partially decomposed peat and create environments for unique assemblages of other plants, animals, and microbes. There are an estimated 250-400 peatmoss species although the exact number of species and their genealogical relationships remain to be tested. This project focuses on species delimitation, estimating genealogical relationships, and producing a new taxonomic classification for one group of peatmosses: Sphagnum subg. Cuspidata. Undergraduates, graduate students and post-doctoral researchers will be trained in diverse field and laboratory techniques and receive hands-on experience working in Sphagnum-dominated peatland ecosystems. A series of workshops and field courses will engage both professional and amateur naturalists in the project, and a new illustrated book will introduce preschool children to the diversity of plant life. All DNA sequence data generated by the project will be deposited in public databases and other research products will be freely accessible.The central aims of this project are to assess species delimitation, phylogenetic relationships, interspecific admixture, and inter- / intra-specific genome structure in Sphagnum, subgenus Cuspidata. Subgenus Cuspidata includes approximately 20-25 species. The project covers a broad range of geographic scales including proximate populations, regional metapopulations, and plants from different continents, and includes three integrated parts. The first aim uses RADSeq to test hypotheses of phylogeographic structure among plants collected in Europe, eastern North America, western North America, Asia, and specifically, whether eastern North American and European plants form a clade sister to plants from western North America and Asia. The RADSeq data will be used to delimit phylogenetic species, including estimates of genetic admixture among species and intraspecific, geographic metapopulation systems. The second aim uses whole genome sequencing to test hypotheses about genomic differentiation among species and geographically disjunct population systems, as well as genomic regions that are shared among species- and population-level clades. A major goal is to assess the physical architecture of shared and differentiated genomic regions across the 19 chromosomes of haploid gametophytes. Comparative analyses will test hypotheses that species differ in genes related to nutrient requirements and water relations, two important niche gradients that differentiate members of Sphagnum subg. Cuspidata. The comparative analyses are facilitated by rich genomic resources available for Sphagnum, including a reference genome and genetic map for a species in subg. Cuspidata (S. fallax). The third and final aim integrates and synthesizes data within a taxonomic revision of the subgenus, including identification keys, descriptions, nomenclatural revisions, illustrations, and distribution maps.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

泥炭藓（泥炭藓属）覆盖了北半球的大片地区，在那里它们有助于调节区域排水模式，在遥远的北方，它们还控制着永久冻土的范围。在这些以泥炭为主的泥炭地生态系统中，泥炭以部分分解的泥炭的形式储存了大量的碳，并为其他植物、动物和微生物的独特组合创造了环境。估计有250-400种泥炭土，但物种的确切数量和它们的谱系关系仍有待检验。本项目主要对泥茅属（Sphagnum subg）进行了种划分、系谱关系估计和新的分类。Cuspidata。本科生、研究生和博士后研究人员将接受不同领域和实验室技术的培训，并获得在以泥炭为主的泥炭地生态系统中工作的实践经验。一系列的研讨会和实地课程将吸引专业和业余博物学家参与该项目，一本新的插图书将向学龄前儿童介绍植物生命的多样性。该项目产生的所有DNA序列数据将存入公共数据库，其他研究产品将免费提供。本研究的主要目的是评估Sphagnum， Cuspidata亚属的物种划分、系统发育关系、种间混合和种间/种内基因组结构。虎蝇亚属包括大约20-25种。该项目涵盖了广泛的地理尺度，包括近缘种群、区域超种群和来自不同大陆的植物，并包括三个完整的部分。第一个目的是使用RADSeq来测试在欧洲、北美东部、北美西部和亚洲收集的植物的系统地理结构假设，特别是北美东部和欧洲的植物是否与北美西部和亚洲的植物形成了一个分支姐妹。RADSeq数据将用于划分系统发育物种，包括物种间遗传混合和种内、地理元种群系统的估计。第二个目标是使用全基因组测序来测试关于物种之间和地理上不相交的种群系统之间的基因组差异的假设，以及物种和种群水平进化枝之间共享的基因组区域。主要目的是评估在单倍体配子体的19条染色体上共享和分化的基因组区域的物理结构。比较分析将验证物种在与营养需求和水关系相关的基因上存在差异的假设，这两个重要的生态位梯度区分了Sphagnum亚科成员。Cuspidata。Sphagnum丰富的基因组资源，包括参考基因组和subg中一个物种的遗传图谱，为比较分析提供了便利。虎蛾属；第三个也是最后一个目标是整合和综合亚属分类修订中的数据，包括识别键、描述、命名修订、插图和分布图。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Morphological-molecular incongruence in Sphagnum majus ssp. majus and ssp. norvegicum

Sphagnum majus ssp 中的形态分子不一致。

• 发表时间: 2022
• 期刊: The Bryologist
• 作者: Marta Nieto;S. Robinson;Blanka Aguero;Aaron M. Duffy;Karn Imwattana;K. Hassel;K. Flatberg;H. Stenøien;A. Shkurko;V. Fedosov;A. J. Shaw
• 通讯作者: A. J. Shaw