Collaborative Research: The brood cell microbiome of solitary bees: origin, diversity, function, and vulnerability

合作研究：独居蜜蜂的巢细胞微生物组：起源、多样性、功能和脆弱性

• 批准号: 1929499
• 负责人: Bryan Danforth
• 金额: $ 61.3万
• 依托单位: Cornell University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1929499&HistoricalAwards=false
• 关键词: Collaborative; Research; brood; cell; microbiome

Bees are the single most important pollinators of flowering plants worldwide. Over 85% of the 325,000 flowering plant species on earth depend on animals for pollination, and the vast majority of pollination is carried out by bees. Annually, bees are estimated to contribute $15 billion to US crop production and $170 billion to global crop production. High-value bee-pollinated crops include apple and other early spring tree fruits, strawberries, blueberries, cherries, cranberries, squash and pumpkins, tomatoes, almonds, and many others. The economic viability of US agricultural production is dependent on stable and healthy wild and domesticated bee populations. However, bee populations are threatened by a variety of factors, including habitat loss, pathogen spillover, invasive plants and animals, and pesticide use, which can disrupt the normal microbial symbionts essential for bee larval development (the "brood cell" microbiome). This research project focuses on understanding what role microbes play in the larval nutrition in a wide variety of bee species. Previous research has documented a diverse community of bacteria and yeasts in the pollen and nectar diet of bees. As larvae consume these pollen/nectar provisions they are ingesting microbes, and our preliminary results indicate that these microbes form an essential component of the larval diet. This project has the potential to significantly modify how we view the 120 million-year-old partnership between bees and flowering plants, and will provide essential information for developing long-term bee conservation efforts. Project outreach efforts include educational activities on solitary bees for K-12 students and interactive demonstrations of bee-microbe-flower interactions for broad audiences. The project will use cutting-edge methods to (1) document the microbial diversity in flowers and pollen provisions, (2) determine the nutritional role of microbes in larval development and health, and (3) understand how alterations in microbial community impact larval development. To document microbial diversity in both host-plant flowers and pollen provisions, the research team will use amplicon sequencing and microbial metagenomics. These methods will document the microbial species present in pollen provisions as well as the metabolic activities these microbes perform during pollen maturation. Screening the pollen and nectar of host-plant species will provide key insights into the source of the brood cell microbiome. To determine the nutritional role of the microbial community the research team will use two methods from trophic ecology: compound specific isotope analysis and neutral lipid fatty acid analysis. These analyses will permit the research team to track the origin (floral or microbial) of amino acids and fatty acids in the larval diet of 15 focal bee species. Finally, through manipulative laboratory experiments the research team will determine how modifications of the microbial communities impact larval development. Combining the results of these studies will provide a comprehensive understanding of how bees and flowering plants interact via their shared microbial partners. This project is jointly funded by the Systematics and Biodiversity Sciences Cluster (Division of Environmental Biology) and the Symbiosis, Defense and Self-recognition Program (Division of Integrative Organismal Systems).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

蜜蜂是全世界开花植物最重要的传粉者。地球上325,000种开花植物中有85%以上依靠动物授粉，而绝大多数授粉是由蜜蜂完成的。据估计，蜜蜂每年为美国作物生产贡献150亿美元，为全球作物生产贡献1700亿美元。高价值的蜜蜂授粉作物包括苹果和其他早春果树、草莓、蓝莓、樱桃、蔓越莓、南瓜和南瓜、西红柿、杏仁等。美国农业生产的经济活力依赖于稳定和健康的野生和家养蜜蜂种群。然而，蜜蜂种群受到多种因素的威胁，包括栖息地丧失、病原体外溢、动植物入侵和农药使用，这些因素可能破坏蜜蜂幼虫发育所必需的正常微生物共生体（“育苗细胞”微生物群）。本研究项目的重点是了解微生物在各种蜜蜂的幼虫营养中所起的作用。以前的研究已经记录了蜜蜂的花粉和花蜜饮食中细菌和酵母的多样化群落。当幼虫消耗这些花粉/花蜜时，它们正在摄入微生物，我们的初步结果表明，这些微生物构成了幼虫饮食的重要组成部分。这个项目有可能极大地改变我们对蜜蜂和开花植物之间1.2亿年前的伙伴关系的看法，并将为发展长期的蜜蜂保护工作提供必要的信息。项目外展工作包括为K-12年级学生举办关于独居蜜蜂的教育活动，以及为广大观众举办蜜蜂-微生物-花朵互动示范。该项目将使用尖端的方法来(1)记录花和花粉供应中的微生物多样性，(2)确定微生物在幼虫发育和健康中的营养作用，以及(3)了解微生物群落的变化如何影响幼虫的发育。为了记录寄主植物花朵和花粉供应中的微生物多样性，研究小组将使用扩增子测序和微生物宏基因组学。这些方法将记录花粉供应中存在的微生物种类以及这些微生物在花粉成熟过程中进行的代谢活动。筛选寄主植物物种的花粉和花蜜将提供对育苗细胞微生物群来源的关键见解。为了确定微生物群落的营养作用，研究小组将使用营养生态学中的两种方法：化合物特定同位素分析和中性脂质脂肪酸分析。这些分析将使研究小组能够追踪15种局灶蜂幼虫饮食中氨基酸和脂肪酸的来源（花或微生物）。最后，通过可操作的实验室实验，研究小组将确定微生物群落的修改如何影响幼虫的发育。结合这些研究的结果将提供一个全面的了解蜜蜂和开花植物如何通过它们共同的微生物伙伴相互作用。本项目由系统学和生物多样性科学集群（环境生物学部）和共生、防御和自我识别项目（综合生物系统学部）共同资助。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Biology of Andrena (Callandrena sensu lato) asteris Robertson (Hymenoptera: Andrenidae), an eastern aster specialist that makes a very deep nest

安德雷纳 (Callandrena sensu lato) asteris Robertson（膜翅目：安德雷尼达科）的生物学，是东部紫苑专家，巢穴非常深

• 发表时间: 2023
• 期刊: Northeastern naturalist
• 影响因子: 0.4
• 作者: Espinoza, A.C.

(More than) Hitchhikers through the network: The shared microbiome of bees and flowers

• DOI: 10.1016/j.cois.2020.09.007
• 发表时间: 2021-04-01
• 期刊: CURRENT OPINION IN INSECT SCIENCE
• 影响因子: 5.3
• 作者: Keller, Alexander;McFrederick, Quinn S.;Leonhardt, Sara D.
• 通讯作者: Leonhardt, Sara D.

Diverse Diets with Consistent Core Microbiome in Wild Bee Pollen Provisions

• DOI: 10.3390/insects11080499
• 发表时间: 2020-08-01
• 期刊: INSECTS
• 影响因子: 3
• 作者: Dew, Rebecca M.;McFrederick, Quinn S.;Rehan, Sandra M.
• 通讯作者: Rehan, Sandra M.

Microbes make the meal: oligolectic bees require microbes within their host pollen to thrive

• DOI: 10.1111/een.12926
• 发表时间: 2020-08-12
• 期刊: ECOLOGICAL ENTOMOLOGY
• 影响因子: 2.2
• 作者: Dharampal, Prarthana S.;Hetherington, Matthew C.;Steffan, Shawn A.
• 通讯作者: Steffan, Shawn A.

Nesting biology of Centris ( Paracentris ) burgdorfi (Apidae: Centridini)

Centris (Paracentris) burgdorfi（蜜蜂科：Centridini）的筑巢生物学

• DOI: 10.1080/00218839.2020.1717760
• 发表时间: 2020
• 期刊: Journal of Apicultural Research
• 影响因子: 1.9
• 作者: Sabino, William O.;Alves-dos-Santos, Isabel;Queiroz, Elisa Pereira;de Faria, Letícia Biral;Papaj, Daniel R.;Buchmann, Stephen L.;da Silva, Cláudia Inês
• 通讯作者: da Silva, Cláudia Inês