Collaborative Research: RoL: The evo-devo of male pregnancy and its effects on the brood pouch microbiome

合作研究：RoL：雄性妊娠的进化-发育及其对育儿袋微生物组的影响

• 批准号: 2015301
• 负责人: William Cresko
• 金额: $ 107.05万
• 依托单位: University of Oregon Eugene
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2015301&HistoricalAwards=false
• 关键词: Collaborative; Research; RoL; evo; devo

The evolution of novel traits can change the way that organisms interact with their environments to survive, grow and reproduce. Deep knowledge of the underlying genes and developmental changes that underly most evolutionary innovations is sparse, as is understanding of the ecological consequences for both the organisms in which novel traits emerged and the organisms with which they interact in communities. A particular gap in understanding is how the evolution of novel traits influences the biodiversity of their associated microbial communities. This project will help fill this gap in our knowledge by studying a remarkable innovation – male pregnancy in seahorses, pipefish and seadragons. This project will include the creation of new genome sequences and detailed studies of the developmental genetic underpinnings of the embryo brooding structures that make male pregnancy possible. The consequences of pouch evolution on the complexity and function of the community microbes in the pouch will also be studied, as well as how this unique host-associated microbiota can affect the fitness of embryos in the pouch. This project will provide research training to high school students, teachers, and undergraduates from underrepresented groups through immersive outreach and targeted support programs. The project will also support training of the next generation of scientists via education of Ph.D. students and postdoctoral scholars. Outreach to general public will be accomplished through public talks and through creation of a museum exhibit on syngnathid biology paired with web resources to support K-12 education.Male pregnancy, accompanied by morphologically diverse embryo brooding structures, is a defining evolutionary innovation in syngnathid fishes. The goal of this project is to build an integrative understanding of the developmental genetic origin of this remarkable syngnathid novelty and its role in mediating multi-level ecological interactions with host-associated microbiota. This project will include production of 19 new annotated reference genomes strategically sampled across the syngnathid lineage, morphogenetic analysis and transcriptional/epigenetic profiling of the developing pouch in a comparative framework that leverages the repeated, independent evolution of complex brooding structures in the family, and analysis of brood pouch biocomplexity as a determinant of pouch-associated microbiome assembly. When complete, this project will provide novel insights into genome structural evolution in syngnathids, identify protein sequence and gene regulation changes involved in brood pouch development, and address whether the evolution of the brooding tissues created specialization in host regulation of microbiota with consequences for brooded progeny. The work will attract new researchers to syngnathids for studies of evolutionary innovation and diversification. The project will provide research training to high school students, teachers, and undergraduates from underrepresented groups, and will support education of graduate students and postdoctoral scholars, including the opportunity to take intense short courses to learn next generation sequencing, bioinformatics, complex statistical analyses, and genome editing. Educational outreach to general public will be accomplished through public lectures by the PIs, and through creation of a museum exhibit on syngnathid biology, which will be paired with an associated web resource directed toward K-12 education.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

新特征的进化可以改变生物体与环境相互作用以生存，生长和繁殖的方式。对大多数进化创新背后的基因和发育变化的深入了解很少，对出现新特征的生物体和与它们在群落中相互作用的生物体的生态后果的理解也很少。理解上的一个特别差距是新性状的进化如何影响其相关微生物群落的生物多样性。该项目将通过研究一项非凡的创新-海马，海龙和海海龙的雄性怀孕来填补我们知识中的这一空白。该项目将包括创建新的基因组序列和详细研究使男性怀孕成为可能的胚胎育雏结构的发育遗传基础。还将研究育儿袋进化对育儿袋中群落微生物的复杂性和功能的影响，以及这种独特的宿主相关微生物群如何影响育儿袋中胚胎的适应性。该项目将通过沉浸式外展和有针对性的支持计划，为来自代表性不足群体的高中生，教师和本科生提供研究培训。该项目还将通过博士教育支持下一代科学家的培训。学生和博士后学者。与公众的联系将通过公开讲座和创建一个关于海龙生物学的博物馆展览以及网络资源来实现，以支持K-12 education.Male怀孕，伴随着形态多样的胚胎育雏结构，是一个定义进化创新的海龙鱼类。该项目的目标是建立一个综合性的理解，这种显着的Syngnathid新奇的发育遗传起源及其在介导与宿主相关的微生物群的多层次生态相互作用的作用。该项目将包括生产19个新的注释参考基因组，战略性地在海龙科谱系中取样，在一个比较框架中对发育中的育儿袋进行形态发生分析和转录/表观遗传分析，该框架利用了家庭中复杂育雏结构的重复，独立进化，以及分析育儿袋生物复杂性作为育儿袋相关微生物组组装的决定因素。完成后，该项目将提供新的见解基因组结构进化的海龙，确定蛋白质序列和基因调控的变化涉及育袋的发展，并解决是否育雏组织的进化创造了专业化的微生物群的宿主调控的后果育雏后代。这项工作将吸引新的研究人员对海龙类进行进化创新和多样化的研究。该项目将为来自代表性不足群体的高中生、教师和本科生提供研究培训，并将支持研究生和博士后学者的教育，包括参加密集的短期课程以学习下一代测序、生物信息学、复杂的统计分析的机会和基因组编辑。通过PI的公开讲座，以及通过创建一个关于海龙生物学的博物馆展览，将与一个针对K-12教育的相关网络资源配对，将完成对公众的教育推广。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Evolutionary divergence of a Hoxa2b hindbrain enhancer in syngnathids mimics results of functional assays

• DOI: 10.1007/s00427-021-00676-x
• 发表时间: 2021-05
• 期刊: Development Genes and Evolution
• 影响因子: 2.4
• 作者: A. Fuiten;W. Cresko