Establishing Quantitative Functional Metrics to Evaluate the Efficacy of Corneal Crosslinking (CXL) Therapeutic Procedures

建立定量功能指标来评估角膜交联 (CXL) 治疗程序的疗效

• 批准号: 1935817
• 负责人: giuliano scarcelli
• 金额: $ 10.61万
• 依托单位: University of Maryland, College Park
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-12-01 至 2020-11-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1935817&HistoricalAwards=false
• 关键词: Establishing; Quantitative; Functional; Metrics; Evaluate

Keratoconus and related ectatic disorders are the leading indication for corneal transplantation and the primary concern of refractive surgery screening in the US. Corneal collagen crosslinking (CXL) has emerged as the only effective treatment to stop keratoconus progression by stiffening the corneal stroma. CXL safety and efficacy is determined by its mechanical outcome, but currently morphological not biomechanical parameters are used to characterize CXL. This proposal aims to establish novel functional metrics to quantify the mechanical outcome of corneal crosslinking (CXL) procedures to facilitate future evaluation of their safety and efficacy. The research is highly synergistic with the FDA collaborator Dr. Ilev, whose lab has been working to develop repeatable and optical radiation safe CXL procedures. To address this need, the lab has developed Brillouin microscopy which enables a CXL test methodology by characterizing corneal stiffness in situ and in vivo with three-dimensional resolution. Our central hypothesis is that the stiffening induced by CXL as evaluated by Brillouin microscopy can be used to predict corneal shape, i.e. visual outcome. To test this hypothesis, they will (1) validate Brillouin-derived mechanical metrics to gold-standard compressive mechanical tests; (2) correlate CXL-induced mechanical changes to cornea morphological behavior by inducing dynamic deformation of the cornea with air-puff; and (3) correlate Brillouin corneal profiles after CXL to morphological changes due to increasing intraocular pressure (IOP).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

圆锥角膜和相关的扩张性疾病是角膜移植的主要适应症，也是美国屈光手术筛查的主要关注点。 角膜胶原交联（CXL）已成为唯一有效的治疗，以阻止圆锥角膜的进展，硬化角膜基质。CXL的安全性和有效性取决于其力学结果，但目前使用形态学而非生物力学参数来表征CXL。 该提案旨在建立新的功能指标，以量化角膜交联（CXL）手术的机械结果，以促进未来对其安全性和有效性的评价。该研究与FDA合作者Ilev博士高度协同，他的实验室一直致力于开发可重复和光学辐射安全的CXL手术。为了满足这一需求，该实验室开发了布里渊显微镜，通过三维分辨率表征原位和体内角膜硬度来实现CXL测试方法。我们的中心假设是，通过布里渊显微镜评估的CXL诱导的硬化可用于预测角膜形状，即视觉结果。为了验证这一假设，他们将（1）验证Brillouin衍生的力学指标与黄金标准压缩力学测试;（2）通过用空气抽吸诱导角膜的动态变形，将CXL诱导的力学变化与角膜形态学行为相关联;以及（3）将CXL后的布里渊角膜轮廓与由于眼内压（IOP）增加而引起的形态学变化相关联该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Detecting Mechanical Anisotropy of the Cornea Using Brillouin Microscopy.

使用布里渊显微镜检测角膜的机械各向异性。

• DOI: 10.1167/tvst.9.7.26
• 发表时间: 2020
• 期刊: Translational vision science & technology
• 影响因子: 3
• 作者: Webb,JoshuaN;Zhang,Hongyuan;SinhaRoy,Abhijit;Randleman,JamesBradley;Scarcelli,Giuliano
• 通讯作者: Scarcelli,Giuliano