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CAREER:Dissecting the Impact of High Temperature on Clock Function and Clock-Controlled Growth Processes in Plants

职业：剖析高温对植物时钟功能和时钟控制生长过程的影响

基本信息

批准号：
1942949
负责人：
Dawn Nagel
金额：
$ 105万
依托单位：
University of California-Riverside
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1942949&HistoricalAwards=false
关键词：
CAREER Dissecting Impact Temperature Clock

项目摘要

Understanding how plants respond to variable environmental temperatures requires a comprehensive understanding of the underlying regulatory pathways. Coordination between important biological processes and external signals is communicated by the circadian clock. The circadian clock is an internal timekeeping machinery that enhances fitness and is found ubiquitously in bacteria, plants, fungi and animals. Both temperature and the clock affect fundamental processes such as growth in plants. For example, crop productivity is affected by even modest temperature increases, depending on the time of day. Through identification of new temperature regulators of clock function or links to clock-controlled growth responses, the project seeks to uncover novel mechanistic insights into how temperature impacts the clock, growth, and resilience. Therefore, the goal of this research is to understand how extreme temperature impacts clock function and growth in plants. Integrated within this proposal is a 10-week summer research class followed by mentor-guided research experiences to promote recruitment and retention of transfer community college students that are members of under-represented minority groups. This module is designed to train and retain transfer students for advanced education and careers in agriculture. The mentored research experience will fill an existing need for research opportunities for under-represented transfer students at the University of California-Riverside. Through interactions in the lab, students will develop a sense of community, along with purpose and professional connections. Completion of this research will have a broad impact on our understanding of the dynamic nature of plant genomes in response to stress and a positive impact on the US workforce and ultimately global food security.Although massive genome reorganization occurs in response to heat stress in plants, the underlying mechanisms involved are not known. The overall hypothesis to be tested is that plant survival under heat stress is directly dependent on the time of day, and that multiple transcriptional regulators are responsible for integrating temperature signals to the clock to modulate timed responses. This project aims to 1) Characterize the role of Heat Shock Factors on clock function under high temperature, 2) Investigate how clock-controlled heat stress responses impinge upon growth-related processes, and 3) Determine how heat stress impacts translation of key clock and clock-controlled genes. The short-term broader impacts of this project will be to increase knowledge of the dynamic nature of plant interaction with the environment, and provide a pipeline for recruitment and retention of under-represented community college transfer students in plant sciences. Students will receive training in molecular biology techniques, genetics, genomics, and bioinformatics approaches, mentoring and teaching, critical thinking skills, and career development through publications and presentations. These skills provide an advantage for admission to competitive graduate programs in STEM or help them to secure research jobs in academic labs or biotech companies of their choice. In the long term, the results will increase understanding of the dynamics of the clock and environmental temperature and the impact on an organism’s behavior and physiology. In addition, the results could contribute to engineering next-generation strategies to improve yield and stress tolerance in plants.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

了解植物如何应对多变的环境温度需要全面了解潜在的调控途径。重要的生物过程和外部信号之间的协调是通过生物钟来传达的。生物钟是一种内部计时机制，可以增强健康，在细菌，植物，真菌和动物中无处不在。温度和生物钟都会影响植物生长等基本过程。例如，根据一天中的不同时间，即使是温和的温度升高也会影响作物产量。通过识别时钟功能的新温度调节器或与时钟控制的生长反应的联系，该项目旨在揭示温度如何影响时钟，生长和恢复力的新机制。因此，本研究的目的是了解极端温度如何影响植物的生物钟功能和生长。该提案中包含一个为期10周的夏季研究班，然后是导师指导的研究经验，以促进招聘和保留那些代表性不足的少数群体成员的转学社区大学生。该模块旨在培训和留住转学生，以接受高等教育和从事农业职业。指导的研究经验将填补在加州大学河滨分校的代表性不足的转学生的研究机会的现有需求。通过在实验室中的互动，学生将发展社区意识，沿着的目的和专业联系。这项研究的完成将对我们理解植物基因组在应对胁迫时的动态性质产生广泛的影响，并对美国劳动力乃至全球粮食安全产生积极影响。尽管植物在应对热胁迫时发生了大规模的基因组重组，但其潜在机制尚不清楚。待测试的总体假设是，植物在热胁迫下的存活直接取决于一天中的时间，并且多个转录调节因子负责将温度信号整合到时钟中以调节定时响应。该项目旨在1）表征高温下热休克因子对时钟功能的作用，2）研究时钟控制的热应激反应如何影响生长相关过程，3）确定热应激如何影响关键时钟和时钟控制基因的翻译。该项目的短期更广泛的影响将是增加植物与环境相互作用的动态性质的知识，并提供一个管道，招聘和保留代表性不足的社区学院转专业学生在植物科学。学生将通过出版物和演讲接受分子生物学技术，遗传学，基因组学和生物信息学方法，指导和教学，批判性思维技能和职业发展方面的培训。这些技能为进入STEM竞争激烈的研究生课程提供了优势，或帮助他们在自己选择的学术实验室或生物技术公司获得研究工作。从长远来看，这些结果将增加对生物钟和环境温度的动态以及对生物体行为和生理的影响的理解。此外，研究结果还有助于设计下一代策略，以提高植物的产量和抗逆性。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。