CAREER: Understanding bacteria encapsulation, proliferation and release in photodegradable hydrogel materials

职业：了解可光降解水凝胶材料中细菌的封装、增殖和释放

• 批准号: 1944791
• 负责人: Ryan Hansen
• 金额: $ 55.52万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2025-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1944791&HistoricalAwards=false
• 关键词: CAREER; Understanding; bacteria; encapsulation; proliferation

Abstract (non-technical)Advancements in microbial biotechnology continue to expand our knowledge of the bacterial world. To fully understand and predict behavior of bacteria, it is necessary to connect its molecular makeup with its observable characteristics. This often requires individual bacteria first be isolated at high purity, which is currently difficult to do. The goal of this NSF CAREER award is to investigate use of photodegradable hydrogel materials for identification and isolation of rare bacteria cells with unique function for molecular analysis. Hydrogels are three-dimensional, water-soluble polymers that can be used to encapsulate collections of cells for observation. A fundamental understanding of both chemical and physical characteristics of hydrogels that lead to stable and effective encapsulation of bacteria will first be established. These hydrogels will then be designed to degrade following exposure to low-energy, near-infrared light unharmful to bacteria, which will allow for release of live, single cells from the hydrogel at any time. The outcome will be a new, materials-based approach to microbial isolation that will be low cost and highly translational to the standard microbiology lab. These hydrogels will have broader application to other emerging areas in microbiology including targeted delivery of therapeutic bacteria to disease sites. The integrated educational goal of the project is to develop global-minded students who are well prepared for interdisciplinary research careers at the interface of materials science and microbiology through the Joint Microbe-Material Scholars (JMMS) program. The JMMS platform will leverage established programs at Kansas State University for engagement and recruitment of underrepresented minority students throughout the state of Kansas and will provide its scholars with international research experiences. This project is jointly funded by the Biomaterials Program of the NSF Division of Materials Research, and the Established Program to Stimulate Competitive Research (EPSCoR).Abstract (technical)Photodegradable hydrogels have been intensively studied for application in tissue engineering and targeted drug delivery. However, these materials are largely unexplored for use in microbiology, but have great potential for on-demand delivery of bacteria as well as for use in low-cost cell isolation methods. These capabilities are critical for advancing our knowledge of the microbial world and for using bacteria in emerging therapeutic applications. The overall goal of this CAREER proposal is to provide a fundamental understanding of hydrogel material properties that provide stable bacteria encapsulation, culture, transport, and release from photodegradable hydrogels with high viability and precision. Step-growth polymerizations that use thiol-Michael addition reactions for bacteria encapsulation into stable, polyethylene glycol-based hydrogels will be identified, and the impact of hydrogel mesh size and elasticity on bacteria proliferation and transport will be investigated. Up-conversion nanoparticles will be integrated into the hydrogel matrix to investigate release of individual bacteria cells using a patterned NIR light source. Finally, these novel materials will be tested for isolation of rare bacteria with viable but nonculturable phenotypes for follow-up molecular analysis. Knowledge gained from these tasks will inform the rational design of hydrogels for isolation of single bacteria with unique function for 'omics'-based analysis and delivery of live, therapeutic bacteria to targeted disease sites. The research will be integrated into the educational plan through the Joint Microbe-Material Scholars program, an educational platform designed to produce global-minded, interdisciplinary students through four components: (1) an international student exchange with collaborators in food microbiology, (2) an external outreach mechanism designed to engage and recruit minority students throughout the state of Kansas, (3) a microbe-material course that uses an interdisciplinary guided-inquiry teaching method, and (4) public engagement. The PI will leverage successful programs at Kansas State University for reaching broader audiences including the Kansas-Louis Stokes Alliance for Minority Participation program, Project IMPACT, and Science on Tap.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

摘要(非技术)微生物生物技术的进步继续扩大我们对细菌世界的认识。为了充分了解和预测细菌的行为，有必要将其分子组成与其可观察到的特征联系起来。这通常要求首先以高纯度分离单个细菌，这在目前是很难做到的。该NSF职业奖的目标是研究使用可光降解的水凝胶材料来鉴定和分离具有独特分子分析功能的稀有细菌细胞。水凝胶是一种三维的、可溶于水的聚合物，可以用来包裹收集的细胞进行观察。首先将建立对水凝胶的化学和物理特性的基本了解，这些特性导致稳定和有效地包裹细菌。然后，这些水凝胶将被设计为在暴露于对细菌无害的低能量近红外光后降解，这将允许在任何时间从水凝胶中释放活的单个细胞。其结果将是一种新的、基于材料的微生物分离方法，这种方法成本低，对标准微生物实验室具有很高的转化性。这些水凝胶将在微生物学的其他新兴领域有更广泛的应用，包括将治疗性细菌定向输送到疾病部位。该项目的综合教育目标是通过联合微生物-材料学者计划(JMMS)培养具有全球意识的学生，他们为材料科学和微生物学领域的跨学科研究生涯做好充分准备。JMMS平台将利用堪萨斯州立大学现有的项目，在堪萨斯州范围内吸引和招收代表不足的少数族裔学生，并将为其学者提供国际研究经验。该项目由美国国家科学基金会材料研究部生物材料计划和已建立的促进竞争研究计划(EPSCoR)共同资助。摘要(技术)光降解水凝胶在组织工程和靶向药物输送中的应用已被广泛研究。然而，这些材料在很大程度上还没有被探索用于微生物学，但在按需输送细菌以及用于低成本的细胞分离方法方面具有巨大的潜力。这些能力对于增进我们对微生物世界的知识以及在新兴的治疗应用中使用细菌至关重要。这份职业建议书的总体目标是提供对水凝胶材料性质的基本了解，这些材料能够以高活性和精确度提供稳定的细菌包裹、培养、运输和从光降解水凝胶中释放。使用硫醇-迈克尔加成反应将细菌包裹到稳定的聚乙二醇基水凝胶中的分步生长聚合将被识别，并将研究水凝胶网目尺寸和弹性对细菌增殖和运输的影响。上转换纳米粒子将被集成到水凝胶基质中，以使用图案化的近红外光源研究单个细菌细胞的释放。最后，这些新材料将被测试以分离出具有活的但不可培养的表型的稀有细菌，用于后续的分子分析。从这些任务中获得的知识将为合理设计水凝胶以分离单个细菌提供信息，该水凝胶具有独特的功能，用于基于组学的分析和将活的治疗性细菌输送到目标疾病部位。这项研究将通过联合微生物-材料学者计划整合到教育计划中，该计划是一个旨在通过四个组成部分培养具有全球视野的跨学科学生的教育平台：(1)与食品微生物学合作者进行的国际学生交流，(2)旨在吸引和招收堪萨斯州各地少数族裔学生的外部外联机制，(3)使用跨学科指导探究教学方法的微生物材料课程，以及(4)公众参与。PI将利用堪萨斯州立大学的成功项目来影响更广泛的受众，包括堪萨斯-路易斯·斯托克斯少数群体参与联盟项目、项目影响和磁带上的科学。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

用于细菌筛选、选择和分离的可光降解水凝胶界面

• DOI: 10.3791/63048
• 发表时间: 2021
• 期刊: Journal of Visualized Experiments
• 作者: Fattahi, Niloufar;Barua, Niloy;van der Vlies, André J.;Hansen, Ryan R.
• 通讯作者: Hansen, Ryan R.

Biofunctionalized Magnetic Nanoparticles with Multiplex Touchdown PCR for Simultaneous and Rapid Detection/Identification of Campylobacter jejuni and Campylobacter coli

• DOI: 10.1155/2022/5104187
• 发表时间: 2022-10-12
• 期刊: JOURNAL OF NANOMATERIALS
• 作者: Wenbap，Pattarapong;Rattanarojpong，Triwit;Tuitemwong，Pravate
• 通讯作者: Tuitemwong，Pravate