CBET: Probing the Effects of Nanoparticle Morphology and Density in Bacterial Adhesion on Bioinspired Nanometallic Arrays.

CBET：探讨纳米颗粒形态和密度对仿生纳米金属阵列细菌粘附的影响。

• 批准号: 1951499
• 负责人: Marco De Jesus
• 金额: $ 45.46万
• 依托单位: University of Puerto Rico Mayaguez
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1951499&HistoricalAwards=false
• 关键词: CBET; Probing; Effects; Nanoparticle; Morphology

Dr. Marco De Jesus and his group at the University of Puerto Rico - Mayaguez will adapt bioinspired replicas of naturally occurring and engineered metal nanostructures to gain a deeper understanding of how bacteria interact with nanoscale surfaces whose morphologies are smaller than microorganisms. The investigators will perform an assessment of the structural parameters which influence bacterial adhesion and colonization to devise new materials with controlled bio-adhesive properties for biomedical and environmental applications. In collaboration with the Center for Nanophase Materials Sciences at Oak Ridge National Laboratory, the team will assess the performance of cellular adhesion and colonization onto metal nanoparticle arrays. Results of these studies have the potential to impact areas of national interest including the construction of advanced biosensors, medical devices, and cellular grafting applications. The project will provide interdisciplinary training and research opportunities to a diverse group of undergraduate and graduate students in nanotechnology, chemical sensing, and bioanalytical chemistry. The project will involve at least two graduate and six undergraduate students.This project will improve our current understanding of how bioactive agents interact with nanomaterials at the liquid/solid interface. Specifically, in collaboration with the Center for Nanophase Materials Sciences at Oak Ridge National Laboratory, Dr. De Jesus and his team will assess how surface morphology and packing density influence the ability of bacterial cells to attach and colonize nanostructured surfaces. The team is particularly interested in the impact of these properties on the sorption and effective binding of bacterial agents towards the development of scalable and transferable nanostructures. These nanostructures can be used to inhibit pathogen proliferation in medical devices and implants and to improve bacterial binding for biosensing and controlled cell adhesion applications. Using competitive binding experiments and multivariate Raman analysis, the team will identify the structural dependence of surface interactions of mixed chemical agents to elucidate surface selectivity and trends for practical quantitation of mixtures. The successful completion of this project will provide new advances toward the rational design of nanomaterials with tailored properties for specific biological applications. The project will provide research and training opportunities for a diverse group of undergraduate and graduate students at the University of Puerto Rico, Mayaguez in nanotechnology applied to biochemical systems, with direct exposure of the graduate students to state-of-the-art facilities at the Center for Nanophase Materials Sciences. This project is jointly funded by the Nanoscale Interactions Program in the Chemical, Bioengineering, Environmental and Transport Systems (CBET) Division and the Established Program to Stimulate Competitive Research (EPSCoR).This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

波多黎各大学的Marco De Jesus博士和他的团队将采用自然发生和工程金属纳米结构的生物灵感复制品，以更深入地了解细菌如何与形态比微生物小的纳米级表面相互作用。 研究人员将对影响细菌粘附和定植的结构参数进行评估，以设计具有受控生物粘附特性的新材料，用于生物医学和环境应用。 该团队与橡树岭国家实验室的纳米相材料科学中心合作，将评估细胞粘附和定植到金属纳米颗粒阵列上的性能。 这些研究的结果有可能影响国家利益的领域，包括先进的生物传感器，医疗设备和细胞移植应用的建设。该项目将为纳米技术，化学传感和生物分析化学的本科生和研究生提供跨学科的培训和研究机会。该项目将涉及至少两名研究生和六名本科生。该项目将提高我们目前对生物活性剂如何与纳米材料在液/固界面相互作用的理解。具体来说，在与橡树岭国家实验室纳米材料科学中心的合作中，De Jesus博士和他的团队将评估表面形态和堆积密度如何影响细菌细胞附着和定植纳米结构表面的能力。 该团队特别感兴趣的是这些特性对细菌制剂的吸附和有效结合的影响，以开发可扩展和可转移的纳米结构。这些纳米结构可用于抑制医疗设备和植入物中的病原体增殖，并改善生物传感和受控细胞粘附应用的细菌结合。利用竞争性结合实验和多变量拉曼分析，该团队将确定混合化学试剂的表面相互作用的结构依赖性，以阐明混合物的实际定量的表面选择性和趋势。该项目的成功完成将为合理设计具有特定生物应用特性的纳米材料提供新的进展。 该项目将为波多黎各大学的本科生和研究生提供研究和培训机会，将纳米技术应用于生物化学系统，研究生将直接接触到纳米材料科学中心的最先进设施。该项目由化学、生物工程、环境和运输系统（CBET）部门的纳米级相互作用计划和刺激竞争研究的既定计划（EPSCoR）共同资助。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。