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Excellence in Research: Biofilm Adhesive and Kinetic Properties Under Hydrodynamic Influences During Early Evolution Stages

卓越的研究：早期进化阶段水动力影响下的生物膜粘附和动力学特性

基本信息

批准号：
2000330
负责人：
Patrick Ymele-Leki
金额：
$ 71.22万
依托单位：
Howard University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2000330&HistoricalAwards=false
关键词：
Excellence Research Biofilm Adhesive Kinetic

项目摘要

Biofilms are aggregates of bacteria. Biofilms play important roles in ecological, industrial, and biomedical processes of national relevance. For example, they are associated with biofouling, where microorganisms attach to surfaces and reduce the performance of machines. In medical fields, biofilms play a key role in the development of antibiotic-resistant microorganisms. Biofilms often form in wet environments under fluid flow. Although a large body of knowledge on biofilm formation exists, little is known about how initial single- and multi-cell interactions and fluid flow influence the growth and final structure of biofilms. This project will analyze the fundamental processes that govern cell interactions within a biofilm in fluid flow environments. The results will improve understanding of the developmental stages of microbial biofilm formation by investigating the bacteria cell responses under flow conditions. This may ultimately lead to a way to control biofilm evolution. These outcomes could also help with novel diagnosis and treatment methods for problems caused by biofilms. This project is a collaboration between researchers at two two Historically Black Colleges and Universities (HBCUs) and one national lab. The institutions are Howard University, Florida A&M University, the National High Magnetic Field Laboratory. The project supports the interdisciplinary training of underrepresented groups. Undergraduate, graduate, and K-12 students in STEM (Science, Technology, Engineering, and Mathematics) will participate at their respective institutions. Biofilms constitute an inherent part of the natural life cycle of most microorganisms, and most bacterial biofilms develop under hydrodynamic stress. The hypothesis is that the cost of growing and evolving in hydrodynamic conditions for these banks of bacteria has a net effect of initiating and promoting intrinsic physical, physiological, and kinetic properties that may contribute to biofilm resistance and resilience. This project presents a novel strategy to investigate the impact of hydrodynamics that occurs within bacterial communities using live and model bacteria systems and high-resolution fluorescent velocimetry. This research integrates theoretical and experimental work with the following objectives: (1) Investigate the various hydrodynamic conditions at the substratum boundary and their effect on the adhesion kinetics and spatial distribution of bacterial cells and (2) Determine the impact of hydrodynamic forces on the intercellular and interspecies interactions during biofilm evolution, and (3) Develop a working model for predicting the kinetics of recruitment and detachment of bacteria to and from biofilms under hydrodynamic milieus.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生物膜是细菌的聚集体。生物膜在国家相关的生态，工业和生物医学过程中发挥着重要作用。例如，它们与生物污垢有关，微生物附着在表面并降低机器性能。在医学领域，生物膜在耐药性微生物的发展中起着关键作用。生物膜通常在流体流动的潮湿环境中形成。虽然大量的知识生物膜的形成存在，鲜为人知的是如何初始的单细胞和多细胞的相互作用和流体流动影响的生长和最终结构的生物膜。这个项目将分析在流体流动环境中生物膜内细胞相互作用的基本过程。研究结果将通过研究流动条件下细菌细胞的反应来提高对微生物生物膜形成发育阶段的理解。这可能最终导致一种控制生物膜进化的方法。这些结果也可以帮助新的诊断和治疗方法，由生物膜引起的问题。该项目是两个历史上黑人学院和大学（HBCU）和一个国家实验室的研究人员之间的合作。这些机构是霍华德大学、佛罗里达A M大学、国家高磁场实验室。该项目支持对代表性不足的群体进行跨学科培训。 STEM（科学，技术，工程和数学）的本科生，研究生和K-12学生将在各自的机构参加。生物膜构成大多数微生物自然生命周期的固有部分，并且大多数细菌生物膜在流体动力学应力下形成。该假设是，这些细菌库在水动力条件下生长和进化的成本具有启动和促进内在物理、生理和动力学特性的净效应，这些特性可能有助于生物膜的抵抗力和弹性。该项目提出了一种新的策略，使用活的和模型细菌系统和高分辨率荧光测速仪来研究细菌群落内发生的流体动力学的影响。本研究将理论和实验工作结合起来，目标如下：（1）研究基质边界处的各种流体动力学条件及其对细菌细胞的粘附动力学和空间分布的影响，以及（2）确定流体动力学对生物膜进化期间细胞间和种间相互作用的影响，以及（3）开发一个工作模型，用于预测流体动力学环境下细菌从生物膜中招募和分离的动力学。该奖项反映了NSF的法定使命，并通过使用基金会的智力价值和更广泛的评估被认为值得支持。影响审查标准。