Joint numerical-experimental investigation of the dynamics of single- and doubly-tethered DNA molecules in shear flow

单链和双链 DNA 分子在剪切流中动力学的联合数值实验研究

• 批准号: 24144116
• 负责人: Professor Dr.-Ing. Nikolaus Andreas Adams
• 金额: --
• 依托单位: Lehrstuhl für Aerodynamik und Strömungsmechanik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2006
• 资助国家: 德国
• 起止时间: 2005-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/24144116?language=en
• 关键词: Joint; numerical; experimental; investigation; dynamics

The main objective of the project is to investigate the micromechanical behaviour of DNA immersed in a hydrodynamic flow by joint numerical and experimental investigations. This topic is highly relevant for the development of novel single-molecule manipulation techniques in biophysics and bionanotechnology where complex DNA-liquid interactions occur. The current project focuses on the following tasks. (i) Based on our previous work, we will develop a validated numerical simulation tool which is able to predict the behavior of tethered DNA molecules immersed in a liquid flow and is suitable for efficient three-dimensional computations of DNA stretching. (ii) We will investigate the micromechanical behavior of individual DNA molecules, tethered at both ends, exposed to a shear flow. The mechanical interaction between DNA and flow along with the corresponding DNA morphology will be predicted quantitatively. (iii) Finally, a more complex system will be considered by introducing DNA-protein interaction. We will investigate if the mechanical response of DNA to hydrodynamic flow can be used for local sensing, leading to the concept of using an immobilized DNA molecule as a mechano-fluidic sensor for single association events. The development of single-molecule concepts is of large importance for future applications in proteomics, genomics and biomedical diagnostics.

该项目的主要目标是通过联合数值和实验研究来研究DNA在流体动力流动中的微观力学行为。这一主题与生物物理学和生物纳米技术中发生复杂DNA-液体相互作用的新型单分子操纵技术的发展高度相关。目前的项目重点是以下几项任务。(I)在我们以前工作的基础上，我们将开发一个经过验证的数值模拟工具，该工具能够预测DNA分子在液体流动中的行为，并适用于高效的DNA拉伸的三维计算。(Ii)我们将研究单个DNA分子在剪切流中的微观力学行为，这些分子在两端都被拴住。DNA与流体之间的力学相互作用以及相应的DNA形态将被定量地预测。(Iii)最后，将考虑通过引入DNA-蛋白质相互作用来建立更复杂的系统。我们将研究DNA对流体动力流动的机械响应是否可以用于局部传感，从而产生将固定化DNA分子用作单一关联事件的机械流体传感器的概念。单分子概念的发展对蛋白质组学、基因组学和生物医学诊断学的未来应用具有重要意义。