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Collaborative Research: Tunable HDX and Ion-Molecule Interactions Using Doped-Gas Ion Mobility-Mass Spectrometry

合作研究：使用掺杂气体离子淌度质谱法进行可调谐 HDX 和离子分子相互作用

基本信息

批准号：
2003042
负责人：
Brian Clowers
金额：
$ 25.55万
依托单位：
Washington State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-08-15 至 2024-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2003042&HistoricalAwards=false
关键词：
Collaborative Research Tunable HDX Ion

项目摘要

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, the research groups of Professor Brian H. Clowers (Washington State University) and Professor Christopher J. Hogan (University of Minnesota) are working to improve the performance and capabilities of ion mobility spectrometry (IMS), a chemical analysis method with applications ranging from rapid screening for explosives in airports to detailed insight into the structure and function of biomolecules associated with healthy and diseased organisms. The team is devising means of using selective interactions with solvent molecules and other gaseous additives within the IMS instrument in order to enhance the ability to separate and identify molecules in complex mixtures. By combining these methods with mass spectrometry (a powerful complementary technique) and new computational tools, the research aims to provide an innovative workflow that is broadly applicable to chemical analysis. Students engaged in this research obtain unique interdisciplinary training enhanced by opportunities to work “out-of-discipline” as part of their studies. Research opportunities are also made available to undergraduate and high school students.Recently, Professors Clowers and Hogan have demonstrated enhanced separation factors through selective interactions between gas-phase ions and organic vapor modifiers introduced into ion mobility systems. They now seek to gain a more detailed understanding of the underlying interactions of ion-vapor complexes in order to predict and enable expanded use of IM-MS and tandem IM experiments. Their approach uses an innovative, multidimensional ion mobility/hydrogen-deuterium exchange mass spectrometry strategy and first-principles modeling of gas-phase ion-neutral interactions and vapor uptake. Primary aims are to determine (1) if thermodynamics of vapor association depend upon chemical functionality in amino acids and simple peptide ions; (2) if the extent of binding can be extrapolated as a characterization tool for larger, multiply charged ions exhibiting known secondary structure; (3) if clustering dynamics quantitatively describe the behavior of ions in high-field and asymmetric waveform experiments; and (4) if molecular dynamics can accurately describe solvated ion transport.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像项目的支持下，Brian H. flowers教授（华盛顿州立大学）和Christopher J. Hogan教授（明尼苏达大学）的研究小组正在努力提高离子迁移谱法（IMS）的性能和能力。一种化学分析方法，应用范围从快速筛选机场爆炸物到详细了解与健康和患病生物体相关的生物分子的结构和功能。该小组正在设计在IMS仪器中使用与溶剂分子和其他气体添加剂选择性相互作用的方法，以提高分离和识别复杂混合物中分子的能力。通过将这些方法与质谱（一种强大的互补技术）和新的计算工具相结合，该研究旨在提供一种广泛适用于化学分析的创新工作流程。参与这项研究的学生获得了独特的跨学科培训，并有机会在学习中进行“非学科”工作。研究机会也提供给本科生和高中生。最近，克洛斯教授和霍根教授通过引入离子迁移系统的气相离子和有机蒸气改性剂之间的选择性相互作用，证明了增强的分离因素。他们现在寻求对离子蒸汽复合物的潜在相互作用有更详细的了解，以便预测和扩大IM- ms和串联IM实验的使用。他们的方法使用了一种创新的多维离子迁移率/氢-氘交换质谱分析策略，以及气相离子中性相互作用和蒸汽吸收的第一性原理建模。主要目的是确定(1)蒸汽缔合的热力学是否依赖于氨基酸和简单肽离子的化学功能；(2)如果结合程度可以推断为具有已知二级结构的更大的、多重带电离子的表征工具；(3)如果聚类动力学定量描述了高场和非对称波形实验中离子的行为；(4)分子动力学能否准确描述溶剂化离子的输运。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。