Novel Implementations, Analytical Targets, and Mechanistic Studies of Negative Ion Electron Capture Dissociation

负离子电子捕获解离的新颖实现、分析目标和机理研究

• 批准号: 2004043
• 负责人: Kristina Hakansson
• 金额: $ 54.99万
• 依托单位: Regents of the University of Michigan - Ann Arbor
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2004043&HistoricalAwards=false
• 关键词: Novel; Implementations; Analytical; Targets; Mechanistic

With support from the Chemical Measurement and Imaging Program in the Division of Chemistry, Professor Kristina Hakansson and her group at the University of Michigan are working to improve our ability to characterize the chemical structure of molecules, including especially biomolecules such as proteins. Such structural analysis is essential to help us understand the function (and dysfunction) of molecules in complex samples, e.g., biofluids, cells, and tissues. The Hakansson approach employs the attachment of electrons to gaseous anions (negatively charged molecules), inducing diagnostic fragmentation of the target molecule in a process termed negative ion electron capture dissociation (niECD). Adding electrons (which bear a negative charge) to anions (which also are negatively charged) is challenging, due to charge repulsion. Having developed this method, Dr. Hakansson is now working to improve our understanding and broaden the applicability and availability of niECD. The research is providing new approaches to biomolecular structural characterization with important implications for drug discovery and enhanced understanding of the molecular basis of living organisms. Students involved gain exposure to highly interdisciplinary research. Dr. Hakansson also works to bring appreciation for these concepts and for broader STEM opportunities to middle school students in an effort to boost interest in the scientific method at a young age. The Hakansson group is exploring novel niECD instrument configurations, including extension to matrix-assisted laser desorption/ionization and nano-electrospray ionization. They are working to implement niECD in a high pressure cell, enabling use with mass analyzers other than Fourier transform ion cyclotron resonance (FT-ICR). In pursuit of improved insight into the fundamental chemistry underlying niECD, they are pursuing two approaches (drift tube ion mobility and cross-sectional areas by FT-ICR, or CRAFTI (cross-sectional areas by FT-ICR) to testing Dr. Hakansson’s recent findings that compact, presumably salt-bridged structures are required for effective niECD of peptides. These efforts are supported by molecular modeling and density functional theory calculations, with a special focus on novel analyte classes such as carbohydrates and lipids, which were recently discovered by the Hakansson group to undergo niECD despite not fitting the previously proposed zwitterion mechanism.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像计划的支持下，密歇根大学的Kristina Hakansson教授和她的团队正在努力提高我们表征分子化学结构的能力，特别是蛋白质等生物分子。这样的结构分析对于帮助我们了解复杂样品中分子的功能(和功能障碍)是必不可少的，例如生物流体、细胞和组织。Hakansson方法利用电子与气态阴离子(负电荷分子)的连接，在称为负离子电子俘获解离(NiECD)的过程中诱导目标分子的诊断碎裂。由于电荷排斥，将电子(带负电荷)添加到阴离子(也带负电荷)是具有挑战性的。在开发了这种方法之后，Hakansson博士现在正在努力提高我们的理解，扩大niECD的适用性和可用性。这项研究正在为生物分子结构表征提供新的方法，对药物发现和加强对活体的分子基础的理解具有重要意义。参与其中的学生可以接触到高度跨学科的研究。哈坎森博士还致力于为中学生带来对这些概念的欣赏和更广泛的STEM机会，以努力提高他们在年轻时对科学方法的兴趣。Hakansson团队正在探索新的niECD仪器配置，包括扩展到基质辅助激光解吸/电离和纳米电喷雾电离。他们正致力于在高压室中实现NiECD，使其能够与傅立叶变换离子回旋共振(FT-ICR)以外的质量分析仪一起使用。为了更深入地了解niECD背后的基础化学，他们采用了两种方法(漂移管离子迁移率和FT-ICR的截面积，或CRAFTI(FT-ICR的截面积))来测试Hakansson博士最近的发现，即有效的NiECD需要紧凑的、可能是盐桥结构的结构。这些努力得到了分子建模和密度泛函理论计算的支持，特别关注于新的分析物类别，如碳水化合物和脂类，Hakansson小组最近发现，尽管不符合之前提出的两性离子机制，但它们仍然经历了niECD。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。