SBIR Phase I: Development of a field diagnostic for the rapid detection of White Spot Syndrome Virus (WSSV) in shrimp aquaculture

SBIR 第一阶段：开发用于快速检测虾养殖中白斑综合症病毒 (WSSV) 的现场诊断方法

• 批准号: 2015009
• 负责人: Mary Larkin
• 金额: $ 22.5万
• 依托单位: GASKIYA DIAGNOSTICS LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2015009&HistoricalAwards=false
• 关键词: SBIR; Phase; Development; field; diagnostic

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase I project is to increase aquaculture productivity by reducing disease impact. Disease costs billions in losses each year to the aquaculture industry and threatens the global food supply. The disease caused by White Spot Syndrome Virus (WSSV) can devastate a shrimp farm and costs over $1 billion in losses each year. Farmers are often unaware of a disease issue until animals show advanced signs of disease or there are mortalities. Laboratory diagnosis is expensive and results take days-to-weeks, which is often far too long for results to be actionable. Early warning of white spot disease can enable farmers to take immediate action to reduce losses, reducing costs and increasing industrial robustness. This project advances a new diagnostic to quickly, easily, and reliably detect WSSV in cultivated shrimp in a new easy-to-use, on-site test.This SBIR Phase I project will advance translation of a novel diagnostic for WSSV in a technology incorporating engineered proteins with specific targets into a paper-based biosensor. Prior studies demonstrated proof-of-concept with a paper-based immunoassay with polymerization-based amplification for detection of protein-based biomarkers for HRP2–the primary protein biomarker of malaria–with sensitivity as low as 70pM. This project will bioengineer thermostable rcSso7d DNA-binding proteins to bind the WSSV target and incorporate them into the paper testing format. Assay sensitivity, reagent composition, and dynamic range will be explored as part of an effort to understand the biological and functionally meaningful limits of the developed assay. Tasks include a WSSV challenge study to evaluate the sampling parameters and detection limits of the prototype in laboratory cultured shrimp. The resulting prototype will rapidly and selectively bind the WSSV target and yield stable, easily interpretable, colorimetric results.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）I期项目的更广泛的影响/商业潜力是通过降低疾病影响来提高水产养殖生产率。疾病每年给水产养殖业带来数十亿美元的损失，并威胁着全球粮食供应。白斑综合症病毒（WSSV）引起的疾病会破坏虾农场，每年造成超过10亿美元的损失。在动物表现出高级疾病迹象或存在死亡之前，农民通常不会意识到疾病问题。实验室诊断很昂贵，结果需要几天的时间，这通常太长了，无法采取行动。白点疾病的预警可以使农民立即采取行动以减少损失，降低成本和增加工业鲁棒性。该项目将在新的易于使用的现场测试中快速，轻松，轻松，轻松，可靠地检测WSSV。该SBIR I阶段项目将推动对WSSV的新型WSSV的新诊断，该技术在技术上纳入了具有特定靶标的技术掺入技术的蛋白质中，将其转化为纸基生物传感器。先前的研究表明，基于纸张的免疫测定法证明了概念概念，并基于聚合的放大，用于检测基于蛋白质的生物标志物对HRP2的生物标志物 - 疟疾的主要蛋白质生物标志物，其敏感性低至70pm。该项目将生物工程器热稳定RCSSO7D DNA结合蛋白结合WSSV靶标并将其纳入纸质测试格式。将探索测定灵敏度，试剂组成和动态范围，作为了解开发测定的生物学和功能有意义的限制的努力的一部分。任务包括一项WSSV挑战研究，以评估实验室培养的虾中原型的采样参数和检测极限。由此产生的原型将迅速，有选择地绑定WSSV目标，并产生稳定的，易于解释的比色结果。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子和更广泛的影响审查标准来通过评估来诚实地支持支持。