MRI: Acquisition of a High Resolution LC/MS/MS to Enable Undergraduate Chemical and Biochemical Research Across Six Disciplines in the Natural and Applied Sciences at Hope College

该奖项由主要研究仪器和化学研究仪器项目支持。霍普学院正在购买一台液相色谱仪、四极杆、飞行时间、串联质谱仪（LC-Q-TOF-MS/MS），以支持Kristin dittenhaferr - reed教授及其同事Jonathan Peterson、Kenneth Brown、Jason Gillmore和Matthew Smith。一般来说，质谱（MS）是用于鉴定和表征复杂基质中少量化学物质的关键分析方法之一。在一个典型的实验中，这些成分流入质谱仪，在那里它们被电离成母离子和母离子的碎片离子，并测量它们的质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带液相色谱仪的质谱仪通过在混合物到达质谱仪之前对其进行分离，提供了额外的结构鉴定能力。在质谱法的飞行时间（TOF）方法中，离子的质荷比是通过已知强度的电场加速离子的方式来确定的。离子的速度取决于质荷比（相同电荷的较重离子速度较低）。测量离子到达已知距离的探测器所需的时间。这个时间取决于离子的速度，因此是它的质量电荷比的量度。从这个比值和已知的实验参数，可以表征离子。此次收购加强了学院和地区的研究基础设施。该仪器通过让不同的学生参与使用这种现代分析技术的研究和研究训练，扩大了参与范围。此次收购为大量本科生提供了通过各种活动（如研究当地流域）进行培训的机会。颁发质谱仪的目的是为了加强各级的研究和教育。该仪器的获得尤其影响了蛋白质、蛋白质翻译后修饰和代谢物的研究。该仪器用于定量和分析脂质。此外，LC-Q-TOF-MS/MS有助于研究具有光活性和电活性的杂芳香化合物的化学结构、合成和机理。质谱仪还用于开展与环境有关的研究，例如与水质和纳米颗粒表面相互作用有关的研究，这些研究有助于了解药物的命运和运输。该奖项反映了美国国家科学基金会的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Hope College is acquiring a liquid chromatograph, quadrupole, time-of-flight, tandem mass spectrometer (LC-Q-TOF-MS/MS)to support Professor Kristin Dittenhafer-Reed and colleagues Jonathan Peterson, Kenneth Brown, Jason Gillmore and Matthew Smith. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. A MS instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. In the time-of-flight (TOF) method of mass spectrometry, the mass-to-charge ratio of an ion is determined by the way in which ions are accelerated by an electric field of known strength. The velocity of the ion depends on the mass-to-charge ratio (heavier ions of the same charge have lower speeds). The time that the ions take to reach a detector at a known distance is measured. This time depends on the velocity of the ion, and therefore is a measure of its mass-to-charge ratio. From this ratio and known experimental parameters, the ion can be characterized. The acquisition strengthens the research infrastructure at the College and regional area. The instrument broadens participation by involving diverse students in research and research training using this modern analytical technique. The acquisition provides training opportunities to a large number of undergraduate students through a variety of activities such as those studying local watersheds.The award of the mass spectrometer is aimed at enhancing research and education at all levels. The instrument acquisition especially impacts studies of proteins, protein post-translational modification, and metabolites. The instrumentation is used for quantifying and analyzing lipids. In addition, the LC-Q-TOF-MS/MS is helpful in studying chemical structures, syntheses, and mechanisms of photo- and electroactive heteroaromatic compounds. The mass spectrometer is also used to carry out studies related to the environment such as those related to water quality and surface interactions of nanoparticles which are helpful to understand the fate and transport of pharmaceuticals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.