MRI: Acquisition of a High Resolution LC/MS/MS to Enable Undergraduate Chemical and Biochemical Research Across Six Disciplines in the Natural and Applied Sciences at Hope College

MRI：购买高分辨率 LC/MS/MS，以支持希望学院自然科学和应用科学六个学科的本科生化学和生化研究

• 批准号: 2017708
• 负责人: Kristin Dittenhafer-Reed
• 金额: $ 32.05万
• 依托单位: Hope College
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2017708&HistoricalAwards=false
• 关键词: MRI; Acquisition; Resolution; LC; MS

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Hope College is acquiring a liquid chromatograph, quadrupole, time-of-flight, tandem mass spectrometer (LC-Q-TOF-MS/MS)to support Professor Kristin Dittenhafer-Reed and colleagues Jonathan Peterson, Kenneth Brown, Jason Gillmore and Matthew Smith. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. A MS instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. In the time-of-flight (TOF) method of mass spectrometry, the mass-to-charge ratio of an ion is determined by the way in which ions are accelerated by an electric field of known strength. The velocity of the ion depends on the mass-to-charge ratio (heavier ions of the same charge have lower speeds). The time that the ions take to reach a detector at a known distance is measured. This time depends on the velocity of the ion, and therefore is a measure of its mass-to-charge ratio. From this ratio and known experimental parameters, the ion can be characterized. The acquisition strengthens the research infrastructure at the College and regional area. The instrument broadens participation by involving diverse students in research and research training using this modern analytical technique. The acquisition provides training opportunities to a large number of undergraduate students through a variety of activities such as those studying local watersheds.The award of the mass spectrometer is aimed at enhancing research and education at all levels. The instrument acquisition especially impacts studies of proteins, protein post-translational modification, and metabolites. The instrumentation is used for quantifying and analyzing lipids. In addition, the LC-Q-TOF-MS/MS is helpful in studying chemical structures, syntheses, and mechanisms of photo- and electroactive heteroaromatic compounds. The mass spectrometer is also used to carry out studies related to the environment such as those related to water quality and surface interactions of nanoparticles which are helpful to understand the fate and transport of pharmaceuticals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项由主要研究仪器和化学研究仪器计划支持。希望学院正在购买液相色谱仪，四极杆，飞行时间，串联质谱仪（LC-Q-TOF-MS/MS），以支持克里斯汀Dittenhafer-Reed教授和同事乔纳森彼得森，肯尼斯布朗，杰森吉尔摩和马修史密斯。一般来说，质谱（MS）是用于识别和表征复杂基质中少量化学物质的关键分析方法之一。在典型的实验中，组分流入质谱仪，在那里它们被电离成母离子及其碎片离子，并测量它们的质量。这种高灵敏度的技术可以检测和确定复杂混合物中分子的结构。带有液相色谱仪的MS仪器通过在化合物到达质谱仪之前分离化合物的混合物来提供额外的结构鉴定能力。在质谱的飞行时间（TOF）方法中，离子的质荷比是通过已知强度的电场加速离子的方式来确定的。离子的速度取决于质荷比（相同电荷的较重离子具有较低的速度）。测量离子到达已知距离处的检测器所花费的时间。这个时间取决于离子的速度，因此是其质荷比的量度。根据该比率和已知的实验参数，可以表征离子。此次收购加强了学院和区域的研究基础设施。该仪器通过利用这一现代分析技术让不同的学生参与研究和研究培训，扩大了参与。此次收购为大量本科生提供了培训机会，通过各种活动，如研究当地流域的活动。授予质谱仪的目的是加强各级研究和教育。仪器的获取特别影响蛋白质、蛋白质翻译后修饰和代谢物的研究。该仪器用于定量和分析脂质。此外，LC-Q-TOF-MS/MS有助于研究光活性和电活性杂芳族化合物的化学结构、合成和机理。质谱仪还可用于开展与环境相关的研究，如水质和纳米颗粒表面相互作用的研究，这些研究有助于了解药物的命运和运输。该奖项反映了NSF的法定使命，并通过使用基金会的知识价值和更广泛的影响审查标准进行评估，被认为值得支持。