MRI: Acquisition of a High Resolution LC/MS/MS to Enable Undergraduate Chemical and Biochemical Research Across Six Disciplines in the Natural and Applied Sciences at Hope College

该奖项由主要研究仪器项目和化学研究仪器项目支持。霍普学院正在购买一台四极飞行时间串联质谱仪(LC-Q-TOF-MS/MS)，以支持克里斯汀·迪滕哈费尔-里德教授及其同事乔纳森·彼得森、肯尼斯·布朗、杰森·吉尔莫和马修·史密斯。通常，质谱学是用于鉴定和表征复杂基质中少量化学物种的关键分析方法之一。在一个典型的实验中，这些组分流入质谱仪，在那里它们被电离成母离子，并测量它的碎片离子及其质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带有液体色谱仪的MS仪器通过在化合物到达质谱计之前将混合物分离来提供额外的结构鉴定能力。在飞行时间(TOF)质谱学方法中，离子的质荷比是通过离子被已知强度的电场加速的方式来确定的。离子的速度取决于质荷比(相同电荷的较重离子的速度较低)。测量离子在已知距离到达探测器所需的时间。这一时间取决于离子的速度，因此是其质荷比的量度。根据这个比率和已知的实验参数，可以对离子进行表征。此次收购加强了学院和地区的研究基础设施。该仪器通过让不同的学生参与研究和使用这种现代分析技术进行研究培训来扩大参与度。这次收购为大量本科生提供培训机会，通过各种活动，如研究当地分水岭的学生。授予质谱仪的目的是加强各级的研究和教育。仪器的获取尤其影响蛋白质、蛋白质翻译后修饰和代谢物的研究。该仪器用于脂类的定量和分析。此外，LC-Q-TOF-MS/MS有助于研究光活性和电活性杂环化合物的化学结构、合成和机理。质谱仪还用于开展与环境相关的研究，如与水质和纳米颗粒表面相互作用有关的研究，有助于了解药物的命运和运输。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。

This award is supported by the Major Research Instrumentation and the Chemistry Research Instrumentation programs. Hope College is acquiring a liquid chromatograph, quadrupole, time-of-flight, tandem mass spectrometer (LC-Q-TOF-MS/MS)to support Professor Kristin Dittenhafer-Reed and colleagues Jonathan Peterson, Kenneth Brown, Jason Gillmore and Matthew Smith. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components flow into a mass spectrometer where they are ionized into the parent ion and its fragment ions and their masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. A MS instrument with a liquid chromatograph provides additional structural identification power by separating mixtures of compounds before they reach the mass spectrometer. In the time-of-flight (TOF) method of mass spectrometry, the mass-to-charge ratio of an ion is determined by the way in which ions are accelerated by an electric field of known strength. The velocity of the ion depends on the mass-to-charge ratio (heavier ions of the same charge have lower speeds). The time that the ions take to reach a detector at a known distance is measured. This time depends on the velocity of the ion, and therefore is a measure of its mass-to-charge ratio. From this ratio and known experimental parameters, the ion can be characterized. The acquisition strengthens the research infrastructure at the College and regional area. The instrument broadens participation by involving diverse students in research and research training using this modern analytical technique. The acquisition provides training opportunities to a large number of undergraduate students through a variety of activities such as those studying local watersheds.The award of the mass spectrometer is aimed at enhancing research and education at all levels. The instrument acquisition especially impacts studies of proteins, protein post-translational modification, and metabolites. The instrumentation is used for quantifying and analyzing lipids. In addition, the LC-Q-TOF-MS/MS is helpful in studying chemical structures, syntheses, and mechanisms of photo- and electroactive heteroaromatic compounds. The mass spectrometer is also used to carry out studies related to the environment such as those related to water quality and surface interactions of nanoparticles which are helpful to understand the fate and transport of pharmaceuticals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.