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Signaling pathways involved in the phosphorylation of membrane proteins in response to N and C

参与响应 N 和 C 的膜蛋白磷酸化的信号通路

基本信息

批准号：
242598629
负责人：
Professorin Dr. Waltraud Schulze
金额：
--
依托单位：
Fachgebiet Systembiologie der Pflanze (190d)
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2013
资助国家：
德国
起止时间：
2012-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/242598629?language=en
关键词：
Signaling pathways involved phosphorylation membrane

项目摘要

Nitrogen is an essential macronutrient for plant growth. In addition, nitrogen also has signaling functions by triggering specific adaptations in central metabolism and root development. While basic uptake mechanisms for nitrate through plasma membrane located transport systems are well known and global transcriptional responses to changes in nitrogen availability have been studied in the past, the detailed mechanisms in regulation of nitrate uptake, as well as signal transduction events upstream of transcription are still barely revealed. Our work will aim at closing this gap by particularly focusing on studying the regulation of nitrate transporter NRT2.1 and nitrate responsive protein kinases. Phosphorylation is the most well studied post-translational modification with regulatory function. It can induce changes in protein activity, provide docking sites for protein-protein interaction or induce changes in subcellular location. Nitrate transporters were in the past shown to be subject to protein phosphorylation, and especially for NRT1.1 the role of protein phosphorylation in switching of transport affinity has been well described. Also for ammonium transporters, a phosphorylation-dependent activation/inactivation mechanism was elucidated in recent years. Therefore, a particular focus of this project is to study the role of protein phosphorylation for the regulation of the major nitrate transporter NRT2.1.Within the last couple of years, the general picture has emerged that both NO3- and NH4+ transporters are controlled at the protein level by posttranslational modification to ensure fast responses directly at the plasma membrane to highly fluctuating environmental conditions. Therefore, the scientific aim of this collaborative project is (i) to characterize the condition-dependent phosphorylation of target proteins related to N metabolism in response to C and N availability. It will involve both the identification novel C/N regulated proteins as well as the specific characterisation of the role of the known NRT2.1 phosphorylation sites. The project aim at (ii) finding candidate protein kinases and/or phosphatases using both NRT2.1 and selected nitrogen-metabolism related proteins as targets and (iii) characterising in vivo the impact of the interesting protein kinases and/or phosphatases on the phosphorylation of NRT2.1 and selected nitrogen metabolism related proteins. We will also start to investigate the proteins interacting with the selected protein kinases and/or phosphatases as baits. We are convinced that with the complementary expertise of both partner groups and with a clear focus on NRT2.1 regulation on protein level, we will gain novel valuable insights into a crucial plant function, namely the regulation of nitrogen uptake.

氮是植物生长必需的常量营养素。此外，氮还通过触发中央代谢和根系发育的特定适应而具有信号传导功能。虽然硝酸盐通过位于质膜的转运系统的基本吸收机制是众所周知的，并且过去已经研究了对氮可用性变化的全局转录反应，但硝酸盐吸收调节的详细机制以及转录上游的信号转导事件仍然很少被揭示。我们的工作旨在通过特别关注硝酸盐转运蛋白 NRT2.1 和硝酸盐响应蛋白激酶的调节来缩小这一差距。磷酸化是研究最深入的具有调节功能的翻译后修饰。它可以诱导蛋白质活性的变化，为蛋白质-蛋白质相互作用提供对接位点或诱导亚细胞位置的变化。硝酸盐转运蛋白过去被证明会受到蛋白质磷酸化的影响，特别是对于 NRT1.1，蛋白质磷酸化在转运亲和力转换中的作用已得到很好的描述。此外，对于铵转运蛋白，近年来阐明了磷酸化依赖性激活/失活机制。因此，该项目的一个特别重点是研究蛋白质磷酸化在调节主要硝酸盐转运蛋白 NRT2.1 中的作用。在过去几年中，人们普遍认为 NO3- 和 NH4+ 转运蛋白都通过翻译后修饰在蛋白质水平上受到控制，以确保在质膜上直接对高度波动的环境条件做出快速响应。因此，该合作项目的科学目标是（i）表征与氮代谢相关的靶蛋白响应碳和氮可用性的条件依赖性磷酸化。它将涉及新型 C/N 调节蛋白的鉴定以及已知 NRT2.1 磷酸化位点作用的具体表征。该项目旨在 (ii) 使用 NRT2.1 和选定的氮代谢相关蛋白作为靶标寻找候选蛋白激酶和/或磷酸酶，以及 (iii) 在体内表征感兴趣的蛋白激酶和/或磷酸酶对 NRT2.1 和选定的氮代谢相关蛋白磷酸化的影响。我们还将开始研究与作为诱饵的选定蛋白激酶和/或磷酸酶相互作用的蛋白质。我们相信，凭借两个合作伙伴团队的互补专业知识，并明确关注 NRT2.1 对蛋白质水平的调节，我们将对重要的植物功能（即氮吸收的调节）获得新的有价值的见解。