Impact of NRT2.1 posttranslational regulatory mechanisms on nitrate uptake and sensing

NRT2.1 翻译后调节机制对硝酸盐吸收和传感的影响

• 批准号: 505598517
• 负责人: Professorin Dr. Waltraud Schulze
• 金额: --
• 依托单位: Fachgebiet Systembiologie der Pflanze (190d)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/505598517?language=en
• 关键词: Impact; NRT2.1; posttranslational; regulatory; mechanisms

Nitrate is an essential macronutrient for plant growth. In addition it has signaling functions triggering specific adaptations in metabolism and root development. In A. thaliana, the transporter NRT2.1 is known to have a central role for root nitrate uptake and it could also play a role in signaling for root development in response to nitrate. Previous work initiated by both partners started to draw a complex picture for NRT2.1 post-translational regulations involving phosphorylation of N- and C-terminus at a total of five different sites, interaction with partner proteins, and cleavage of the C-terminal part. The goal of this project is now to build on the strong complementarity of skills between the German and the French partners and combined proteomics approaches with biochemistry, genetic and plant physiology to decipher these complex regulatory mechanisms. Specifically, the project aims at (i) understanding the dynamics of protein-protein interactions of NRT2.1 depending on its modification status, (ii) identification of further regulatory proteins (kinases, phosphatases) acting on NRT2.1, (iii) deciphering the mechanisms of NRT2.1 C-terminal proteolysis as an additional regulatory mechanism. The project will provide novel and valuable insights into the regulation of root nitrate uptake and/or nitrate sensing, which has a strategic importance to improve plants nitrogen use efficiency.

硝酸盐是植物生长所必需的大量营养元素。此外，它还具有信号功能，可触发代谢和根发育中的特定适应。在拟南芥中，转运体NRT2.1在根系硝酸盐吸收中起着核心作用，它也可能在根系发育响应硝酸盐的信号传导中发挥作用。双方之前的工作开始描绘NRT2.1翻译后调控的复杂图景，涉及总共五个不同位点的N和c端磷酸化，与伴侣蛋白的相互作用以及c端部分的切割。该项目的目标是建立在德国和法国合作伙伴之间强大的技术互补基础上，并将蛋白质组学方法与生物化学、遗传和植物生理学相结合，以破译这些复杂的调节机制。具体而言，该项目旨在(i)了解NRT2.1根据其修饰状态的蛋白质-蛋白质相互作用的动力学，（ii）鉴定作用于NRT2.1的进一步调节蛋白（激酶，磷酸酶），（iii）破译NRT2.1 c端蛋白水解的机制，作为额外的调节机制。该项目将为植物根系硝酸盐吸收和/或硝酸盐感知的调控提供新的和有价值的见解，这对提高植物氮素利用效率具有战略意义。