Impact of NRT2.1 posttranslational regulatory mechanisms on nitrate uptake and sensing

NRT2.1 翻译后调节机制对硝酸盐吸收和传感的影响

• 批准号: 505598517
• 负责人: Professorin Dr. Waltraud Schulze
• 金额: --
• 依托单位: Fachgebiet Systembiologie der Pflanze (190d)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/505598517?language=en
• 关键词: Impact; NRT2.1; posttranslational; regulatory; mechanisms

Nitrate is an essential macronutrient for plant growth. In addition it has signaling functions triggering specific adaptations in metabolism and root development. In A. thaliana, the transporter NRT2.1 is known to have a central role for root nitrate uptake and it could also play a role in signaling for root development in response to nitrate. Previous work initiated by both partners started to draw a complex picture for NRT2.1 post-translational regulations involving phosphorylation of N- and C-terminus at a total of five different sites, interaction with partner proteins, and cleavage of the C-terminal part. The goal of this project is now to build on the strong complementarity of skills between the German and the French partners and combined proteomics approaches with biochemistry, genetic and plant physiology to decipher these complex regulatory mechanisms. Specifically, the project aims at (i) understanding the dynamics of protein-protein interactions of NRT2.1 depending on its modification status, (ii) identification of further regulatory proteins (kinases, phosphatases) acting on NRT2.1, (iii) deciphering the mechanisms of NRT2.1 C-terminal proteolysis as an additional regulatory mechanism. The project will provide novel and valuable insights into the regulation of root nitrate uptake and/or nitrate sensing, which has a strategic importance to improve plants nitrogen use efficiency.

硝酸盐是植物生长的必需大量营养素。此外，它具有信号函数，触发了代谢和根源发展中的特定适应性。在A. thaliana中，已知转运蛋白NRT2.1在根硝酸盐摄取中具有核心作用，并且还可以在响应硝酸盐的根发育信号中发挥作用。双方发起的先前的工作开始为NRT2.1翻译后法规绘制复杂的图片，涉及在总共五个不同地点的N-和C端磷酸化，与伴侣蛋白的相互作用以及C-末端部分的裂解。该项目的目的是基于德国与法国伙伴之间的技能互补性，并结合了蛋白质组学方法与生物化学，遗传和植物生理学，以破译这些复杂的调节机制。具体而言，该项目的目的是（i）理解NRT2.1蛋白质蛋白相互作用的动力学，具体取决于其修饰状态，（ii）鉴定作用于NRT2.1，（III）识别NRT2.1 c- termalnical蛋白质蛋白质蛋白质的机制的进一步调节蛋白（激酶，磷酸酶）作为另一种调节性的机制。该项目将对根硝酸盐摄取和/或硝酸盐传感的调节提供新颖而有价值的见解，这对于提高植物氮的使用效率具有战略重要性。