Posttranslational regulatory modules of carbon partitioning and allocation

碳分配和翻译后调控模块

• 批准号: 253569436
• 负责人: Professorin Dr. Waltraud Schulze
• 金额: --
• 依托单位: Fachgebiet Systembiologie der Pflanze (190d)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2014
• 资助国家: 德国
• 起止时间: 2013-12-31 至 2018-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/253569436?language=en
• 关键词: Posttranslational; regulatory; modules; carbon; partitioning

Plants as sessile organisms are able to rapidly adapt to altering carbon availability throughout the diurnal cycle. Thereby, allocation of resources to growing sink organs involving membrane transporters is tightly integrated with metabolic and storage functions. This precise regulation requires tight and integrated control of metabolic activities and membrane transport. In the past years, phosphorylation sites and their dynamics of proteins in central carbon and sucrose metabolism as well as for respective transport were identified, but existing knowledge about the role of these modifications in modulation of metabolism, storage and transport is still limited. The major aim of this project is to gain further detailed insights into the role of protein phosphorylation in context of fluctuations in sucrose availability from extracellular or intracellular sources. In the proposed experiments, we will use different mutants with altered intrinsic sucrose status. We aim at (i) quantitatively monitoring protein phosphorylation of key proteins with sucrose-related responses in targeted assays under conditions of different external and internal sucrose availability and relate it to protein activities, (ii) studying subcompartmental distribution of membrane proteins in the respective mutants under different sucrose status, and (iii) verifying selected sucrose-related kinase-substrate relationships. Overall, the project will gain novel insights into on how plants use posttranslational regulation to integratedly respond to different requirements for carbon usage under rapidly changing external conditions.

植物作为无柄生物可以在整个昼夜周期中迅速适应碳的可用性。因此，将资源分配给涉及膜转运蛋白的水槽器官与代谢和储存功能紧密整合在一起。这种确切的调节需要对代谢活动和膜运输的严格控制。在过去的几年中，确定了磷酸化位点及其蛋白质的动力学在中央碳和蔗糖代谢中以及各自的运输中，但是有关这些修饰在调节代谢，存储和运输调制中的作用的现有知识仍然受到限制。该项目的主要目的是进一步详细介绍蛋白质磷酸化在细胞外或细胞内源来蔗糖可用性中的作用中的作用。在拟议的实验中，我们将使用具有内在蔗糖状态改变的不同突变体。 We aim at (i) quantitatively monitoring protein phosphorylation of key proteins with sucrose-related responses in targeted assays under conditions of different external and internal sucrose availability and relate it to protein activities, (ii) studying subcompartmental distribution of membrane proteins in the respective mutants under different sucrose status, and (iii) verifying selected sucrose-related kinase-substrate relationships.总体而言，该项目将获得有关植物如何使用翻译后调节以在迅速变化的外部条件下对碳使用的不同需求的综合性的新见解。

项目成果

Sucrose-induced Receptor Kinase 1 is Modulated by an Interacting Kinase with Short Extracellular Domain

• DOI: 10.1074/mcp.ra119.001336
• 发表时间: 2019-05
• 期刊: Molecular & Cellular Proteomics : MCP
• 作者: X. Wu;Liang-Cui Chu;Lin Xi;Heidi Pertl-Obermeyer;Zhi Li;K. Skłodowski;Clara Sánchez-Rodríguez;G. Obermeyer;W. Schulze